LOCUS       sequence-358064-        8777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knock in SNAPtag C-terminal to human CRY1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Gabriel CH, Del Olmo M, Zehtabian A, Jager M, Reischl S, van Dijk H,
            Ulbricht C, Rakhymzhan A, Korte T, Koller B, Grudziecki A, Maier B, 
            Herrmann A, Niesner R, Zemojtel T, Ewers H, Granada AE, Herzel H, 
            Kramer A
  TITLE     Live-cell imaging of circadian clock protein dynamics in 
            CRISPR-generated knock-in cells.
  JOURNAL   Nat Commun. 2021 Jun 18;12(1):3796. doi: 10.1038/s41467-021-24086-9.
  PUBMED    34145278
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-18"; volume: "12(1):3796. doi"; pages: " 
            10.1038/s41467-021-24086-9"
COMMENT     Sequence Label: pDB-hCRY1-SNAP-CD4-bla
FEATURES             Location/Qualifiers
     source          1..8777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    1060..1107
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1114..1656
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             1663..1680
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1690..1713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1742..1775
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(1783..1816)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1899..1919)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1904..2234
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2085..2220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2147..2166
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2284..2293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2290..2292,2293..2295,2296..3006)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2335..2356)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2596..2615)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2943..2964
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3022..3078
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3085..3480
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3492..3613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3529..3548)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3583..3602
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3641..3674
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(3675..3708)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4669..4872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4823..4843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4869..4893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(4967..5041,5042..6238)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(6556..6572)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6556..6572)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6569..6591)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6580..6596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6604..6610,6611..6628,6629..6634))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6649..6670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6787..6804)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6958..7546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7038..7057)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7717..8508,8509..8577))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8340..8359
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8578..8682)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8750..8768
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta aggtttaaac tcatattttt ccataaactg gacacactga
      241 cagaaatgtt tccctaaaac atacttctct gttctagcca acctattctc ctataatagt
      301 gtttgttttt agaagggttg ataggattgc ttaggctagg taactgccac atagtctgtg
      361 tcttctgctt gtcgtatgat ttgaattgtt taactcatcg ggcaggccct tattccctta
      421 tcctcaagga aatatgtttg gaggagctct gaaggaaaag cgtgggaaaa gggacaagag
      481 tgagggagga atgtctatct cactcttgcc cctatgtttt accttcatgg tcaagtttca
      541 aattgtcatc tagtaaggaa atgtttttta gaaaatgatc atcaatgatt tttcctttga
      601 gctgtcaaca cttctgtgag cctaaattta agttttcctg gtttgagcat gtgatttttg
      661 gttcatagga agattgagat ttagtcttaa aagcatataa atgaccttaa gtacaagcgc
      721 taaaagttgg atttgtagac ttaataatac atacatcttg gatttgattt taagtaattt
      781 tactgtgttt taaatactaa caggttgctc tcaagggagt ggtattttac actatgctca
      841 tggcgacagt cagcaaactc acctgttgaa gcaaggtaag aatgaagcat tggagcatac
      901 tgttcttttt ccttttccta tcttaaacat acatttttta aatgtgcagg aagaagctcc
      961 atgggcactg gtctcagtgg tgggaaacgt cctagtcaag aagaagacac acagagtatt
     1021 ggtcctaaag tccagagaca gagcactaat agcggccgcg aagttcctat tccgaagttc
     1081 ctattctcta gaaagtatag gaacttcacc ggtgacaaag actgcgaaat gaagcgcacc
     1141 accctggata gccctctggg caagctggaa ctgtctgggt gcgaacaggg cctgcaccgt
     1201 atcatcttcc tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc tgccccagcc
     1261 gccgtgctgg gcggaccaga gccactgatg caggccaccg cctggctcaa cgcctacttt
     1321 caccagcctg aggccatcga ggagttccct gtgccagccc tgcaccaccc agtgttccag
     1381 caggagagct ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa gttcggagag
     1441 gtcatcagct acagccacct ggccgccctg gccggcaatc ccgccgccac cgccgccgtg
     1501 aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc cctgccaccg ggtggtgcag
     1561 ggcgacctgg acgtgggggg ctacgagggc gggctcgccg tgaaagagtg gctgctggcc
     1621 cacgagggcc acagactggg caagcctggg ctgggtacta gtcaccatca ccatcaccat
     1681 ggtagcggtg actacaaaga cgatgacgac aaggctagca aagatctgaa gttcctattc
     1741 cgaagttcct attcttcaaa tagtatagga acttcagatc taataacttc gtatagcata
     1801 cattatacga agttattttg aataaaatat ctttattttc attacatctg tgtgttggtt
     1861 ttttgtgttg agctgattta acaaaaattt aacgctgtgg aatgtgtgtc agttagggtg
     1921 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     1981 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2041 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     2101 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     2161 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     2221 taggcttttg caaaaagcta ttgtatatcc attttcggat ctgatcagca ctgagcaggt
     2281 accgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2341 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2401 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2461 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     2521 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2581 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2641 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2701 ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag
     2761 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     2821 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2881 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     2941 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3001 tacaaggaat tcggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     3061 gaggagaacc ctggacctca cgtggccaag cctttgtctc aagaagaatc caccctcatt
     3121 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     3181 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     3241 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     3301 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     3361 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     3421 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     3481 ctcgagaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3541 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3601 tcaatgtatc ttatcatgtc tgtatagaag ttcctattcc gaagttccta ttcttcaaat
     3661 agtataggaa cttcataact tcgtatagca tacattatac gaagttattt gattaattaa
     3721 gcgatcgctt cgaatgcact aattaggtaa atattttaga gctgtatttc ttgctttaga
     3781 agagtatata attaacataa attaagataa tttcaaaaat ggagcaaatc tctattttca
     3841 aaccagaaaa tcttgaggca ttaattttta agcaattttt acaaactcag ttaatttttg
     3901 gtcaagagac atgcatctgt actggagaaa ttgttgcacc agttttatat tcatctgaac
     3961 caatgctctt taaattagag atgtttatga ttttgtggtc aagttttttc ttagaaaaag
     4021 acaacttttt tatttcctta ctatgtaact atgagtctaa aacaattaaa gtggcctgtt
     4081 tattttagtg acattaatat aatcttttta tgaactttcc cctaaatctt tgcctcttaa
     4141 atgttgataa atttctttta tctgttatga tgcttctaaa atctaaatta tttccaattt
     4201 gggaatagtt caaaattttt taaaatgctg gcccttatta gaagtatcag aaagccttgc
     4261 ctgcattcaa tttaattgga tttgggatgt cattttgtga tttaaattaa tatgaaaaat
     4321 atttatacgt tggatttgcc agtttttaaa aattttctgt ttcttcagtt tctaacactg
     4381 tttacatttt ttattgctta gtttttttat gtcaacctaa ttagactata agtatcttga
     4441 agataaggtc aataaacact catcacattt ttgtcattga attatttgca atcaagcttt
     4501 acctagtttt tttttccccc ttaaatcaca gaaaacattc aggaggaata ctggtcgaca
     4561 agcttgcatg cctgcaggtc aattccctgg cattatgccc agtacatgac cttatgggac
     4621 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     4681 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     4741 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     4801 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     4861 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     4921 gacctccata gaagacaccg ggaccgatcc agcctgggga tccaccatga accggggagt
     4981 cccttttagg cacttgcttc tggtgctgca actggcgctc ctcccagcag ccactcaggg
     5041 aaagaaagtg gtgctgggca aaaaagggga tacagtggaa ctgacctgta cagcttccca
     5101 gaagaagagc atacaattcc actggaaaaa ctccaaccag ataaagattc tgggaaatca
     5161 gggctccttc ttaactaaag gtccatccaa gctgaatgat cgcgctgact caagaagaag
     5221 cctttgggac caaggaaact tccccctgat catcaagaat cttaagatag aagactcaga
     5281 tacttacatc tgtgaagtgg aggaccagaa ggaggaggtg caattgctag tgttcggatt
     5341 gactgccaac tctgacaccc acctgcttca ggggcagagc ctgaccctga ccttggagag
     5401 cccccctggt agtagcccct cagtgcaatg taggagtcca aggggtaaaa acatacaggg
     5461 ggggaagacc ctctccgtgt ctcagctgga gctccaggat agtggcacct ggacatgcac
     5521 tgtcttgcag aaccagaaga aggtggagtt caaaatagac atcgtggtgc tagctttcca
     5581 gaaggcctcc agcatagtct ataagaaaga gggggaacag gtggagttct ccttcccact
     5641 cgcctttaca gttgaaaagc tgacgggcag tggcgagctg tggtggcagg cggagagggc
     5701 ttcctcctcc aagtcttgga tcacctttga cctgaagaac aaggaagtgt ctgtaaaacg
     5761 ggttacccag gaccctaagc tccagatggg caagaagctc ccgctccacc tcaccctgcc
     5821 ccaggccttg cctcagtatg ctggctctgg aaacctcacc ctggcccttg aagcgaaaac
     5881 aggaaagttg catcaggaag tgaacctggt ggtgatgaga gccactcagc tccagaaaaa
     5941 tttgacctgt gaggtgtggg gacccacctc ccctaagctg atgctgagct tgaaactgga
     6001 gaacaaggag gcaaaggtct cgaagcggga gaaggcggtg tgggtgctga accctgaggc
     6061 ggggatgtgg cagtgtctgc tgagtgactc gggacaggtc ctgctggaat ccaacatcaa
     6121 ggttctgccc acatggtcga ccccggtgca gccaatggcc ctgattgtgc tggggggcgt
     6181 cgccggcctc ctgcttttca ttgggctagg catcttcttc tgtgtcaggt gccggcactg
     6241 aaggcgacct gcggatccca cgtcactatt gtatactcta tattatactc tatgttatac
     6301 tctgtaatcc tactcaataa acgtgtcacg cctgtgaaac cgtactaagt ctcccgtgtc
     6361 ttcttatcac catcaggtga catcctcgcc caggctgtca atcatgccgg tatcgattcc
     6421 agtagcaccg gccccacgct gacaacccac tcttgcagcg ttagcagcgc ccctcttaac
     6481 aagccgaccc ccaccagcgt cgcggttact aacactcctc tccccgacct gcagcccaag
     6541 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6601 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6661 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6721 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6781 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6961 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7381 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7681 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7741 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7801 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7861 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7921 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7981 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8041 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8101 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8161 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8221 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8281 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8341 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8401 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8461 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8521 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8701 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8761 cacgaggccc tttcgtc
//
