LOCUS       sequence-358066-        8948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector to knock in mClover3 C-terminal to human CRY1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Gabriel CH, Del Olmo M, Zehtabian A, Jager M, Reischl S, van Dijk H,
            Ulbricht C, Rakhymzhan A, Korte T, Koller B, Grudziecki A, Maier B, 
            Herrmann A, Niesner R, Zemojtel T, Ewers H, Granada AE, Herzel H, 
            Kramer A
  TITLE     Live-cell imaging of circadian clock protein dynamics in 
            CRISPR-generated knock-in cells.
  JOURNAL   Nat Commun. 2021 Jun 18;12(1):3796. doi: 10.1038/s41467-021-24086-9.
  PUBMED    34145278
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-18"; volume: "12(1):3796. doi"; pages: " 
            10.1038/s41467-021-24086-9"
COMMENT     Sequence Label: pDB-hCRY1-mClover3-CD4-bla
FEATURES             Location/Qualifiers
     source          1..8948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    1060..1107
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             join(1114..1116,1117..1827)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1834..1851
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1861..1884
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1913..1946
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(1954..1987)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2070..2090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2075..2405
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2256..2391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2318..2337
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      2455..2464
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2461..2463,2464..2466,2467..3177)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2767..2786)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3193..3249
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3256..3651
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3663..3784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3700..3719)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3754..3773
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3812..3845
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(3846..3879)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4840..5043
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4994..5014
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5040..5064
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(5138..5212,5213..6409)
                     /codon_start=1
                     /product="truncated human CD4 cell surface glycoprotein 
                     lacking the cytoplasmic tail"
                     /label=CD4 (truncated)
                     /translation="MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQ
                     KKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDS
                     DTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNI
                     QGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFS
                     FPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLH
                     LTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLML
                     SLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMAL
                     IVLGGVAGLLLFIGLGIFFCVRCRH"
     primer_bind     complement(6727..6743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6727..6743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6740..6762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6751..6767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6775..6781,6782..6799,6800..6805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6820..6841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6958..6975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7129..7717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7209..7228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7888..8679,8680..8748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8511..8530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8749..8853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8921..8939
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta aggtttaaac tcatattttt ccataaactg gacacactga
      241 cagaaatgtt tccctaaaac atacttctct gttctagcca acctattctc ctataatagt
      301 gtttgttttt agaagggttg ataggattgc ttaggctagg taactgccac atagtctgtg
      361 tcttctgctt gtcgtatgat ttgaattgtt taactcatcg ggcaggccct tattccctta
      421 tcctcaagga aatatgtttg gaggagctct gaaggaaaag cgtgggaaaa gggacaagag
      481 tgagggagga atgtctatct cactcttgcc cctatgtttt accttcatgg tcaagtttca
      541 aattgtcatc tagtaaggaa atgtttttta gaaaatgatc atcaatgatt tttcctttga
      601 gctgtcaaca cttctgtgag cctaaattta agttttcctg gtttgagcat gtgatttttg
      661 gttcatagga agattgagat ttagtcttaa aagcatataa atgaccttaa gtacaagcgc
      721 taaaagttgg atttgtagac ttaataatac atacatcttg gatttgattt taagtaattt
      781 tactgtgttt taaatactaa caggttgctc tcaagggagt ggtattttac actatgctca
      841 tggcgacagt cagcaaactc acctgttgaa gcaaggtaag aatgaagcat tggagcatac
      901 tgttcttttt ccttttccta tcttaaacat acatttttta aatgtgcagg aagaagctcc
      961 atgggcactg gtctcagtgg tgggaaacgt cctagtcaag aagaagacac acagagtatt
     1021 ggtcctaaag tccagagaca gagcactaat agcggccgcg aagttcctat tccgaagttc
     1081 ctattctcta gaaagtatag gaacttcacc ggtgtgagca agggcgagga gctgttcacc
     1141 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtc
     1201 cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
     1261 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcgtggcc
     1321 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1381 gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta caagacccgc
     1441 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1501 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa cagccactac
     1561 gtctatatca cggccgacaa gcagaagaac tgcatcaagg ctaacttcaa gatccgccac
     1621 aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1681 gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccaa gctgagcaaa
     1741 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatt
     1801 acacatggca tggacgagct gtacaagact agtcaccatc accatcacca tggtagcggt
     1861 gactacaaag acgatgacga caaggctagc aaagatctga agttcctatt ccgaagttcc
     1921 tattcttcaa atagtatagg aacttcagat ctaataactt cgtatagcat acattatacg
     1981 aagttatttt gaataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtt
     2041 gagctgattt aacaaaaatt taacgctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2101 cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2161 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2221 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2281 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2341 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     2401 gcaaaaagct attgtatatc cattttcgga tctgatcagc actgagcagg taccgccacc
     2461 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2521 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     2581 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2641 ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     2701 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     2761 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2821 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2881 aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa gcagaagaac
     2941 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3001 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3061 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3121 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggaa
     3181 ttcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     3241 cctggacctc acgtggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca
     3301 acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc
     3361 tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt
     3421 gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc
     3481 gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg
     3541 cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg
     3601 gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta actcgagacc
     3661 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3721 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3781 cttatcatgt ctgtatagaa gttcctattc cgaagttcct attcttcaaa tagtatagga
     3841 acttcataac ttcgtatagc atacattata cgaagttatt tgattaatta agcgatcgct
     3901 tcgaatgcac taattaggta aatattttag agctgtattt cttgctttag aagagtatat
     3961 aattaacata aattaagata atttcaaaaa tggagcaaat ctctattttc aaaccagaaa
     4021 atcttgaggc attaattttt aagcaatttt tacaaactca gttaattttt ggtcaagaga
     4081 catgcatctg tactggagaa attgttgcac cagttttata ttcatctgaa ccaatgctct
     4141 ttaaattaga gatgtttatg attttgtggt caagtttttt cttagaaaaa gacaactttt
     4201 ttatttcctt actatgtaac tatgagtcta aaacaattaa agtggcctgt ttattttagt
     4261 gacattaata taatcttttt atgaactttc ccctaaatct ttgcctctta aatgttgata
     4321 aatttctttt atctgttatg atgcttctaa aatctaaatt atttccaatt tgggaatagt
     4381 tcaaaatttt ttaaaatgct ggcccttatt agaagtatca gaaagccttg cctgcattca
     4441 atttaattgg atttgggatg tcattttgtg atttaaatta atatgaaaaa tatttatacg
     4501 ttggatttgc cagtttttaa aaattttctg tttcttcagt ttctaacact gtttacattt
     4561 tttattgctt agttttttta tgtcaaccta attagactat aagtatcttg aagataaggt
     4621 caataaacac tcatcacatt tttgtcattg aattatttgc aatcaagctt tacctagttt
     4681 ttttttcccc cttaaatcac agaaaacatt caggaggaat actggtcgac aagcttgcat
     4741 gcctgcaggt caattccctg gcattatgcc cagtacatga ccttatggga ctttcctact
     4801 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4861 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4921 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4981 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     5041 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     5101 agaagacacc gggaccgatc cagcctgggg atccaccatg aaccggggag tcccttttag
     5161 gcacttgctt ctggtgctgc aactggcgct cctcccagca gccactcagg gaaagaaagt
     5221 ggtgctgggc aaaaaagggg atacagtgga actgacctgt acagcttccc agaagaagag
     5281 catacaattc cactggaaaa actccaacca gataaagatt ctgggaaatc agggctcctt
     5341 cttaactaaa ggtccatcca agctgaatga tcgcgctgac tcaagaagaa gcctttggga
     5401 ccaaggaaac ttccccctga tcatcaagaa tcttaagata gaagactcag atacttacat
     5461 ctgtgaagtg gaggaccaga aggaggaggt gcaattgcta gtgttcggat tgactgccaa
     5521 ctctgacacc cacctgcttc aggggcagag cctgaccctg accttggaga gcccccctgg
     5581 tagtagcccc tcagtgcaat gtaggagtcc aaggggtaaa aacatacagg gggggaagac
     5641 cctctccgtg tctcagctgg agctccagga tagtggcacc tggacatgca ctgtcttgca
     5701 gaaccagaag aaggtggagt tcaaaataga catcgtggtg ctagctttcc agaaggcctc
     5761 cagcatagtc tataagaaag agggggaaca ggtggagttc tccttcccac tcgcctttac
     5821 agttgaaaag ctgacgggca gtggcgagct gtggtggcag gcggagaggg cttcctcctc
     5881 caagtcttgg atcacctttg acctgaagaa caaggaagtg tctgtaaaac gggttaccca
     5941 ggaccctaag ctccagatgg gcaagaagct cccgctccac ctcaccctgc cccaggcctt
     6001 gcctcagtat gctggctctg gaaacctcac cctggccctt gaagcgaaaa caggaaagtt
     6061 gcatcaggaa gtgaacctgg tggtgatgag agccactcag ctccagaaaa atttgacctg
     6121 tgaggtgtgg ggacccacct cccctaagct gatgctgagc ttgaaactgg agaacaagga
     6181 ggcaaaggtc tcgaagcggg agaaggcggt gtgggtgctg aaccctgagg cggggatgtg
     6241 gcagtgtctg ctgagtgact cgggacaggt cctgctggaa tccaacatca aggttctgcc
     6301 cacatggtcg accccggtgc agccaatggc cctgattgtg ctggggggcg tcgccggcct
     6361 cctgcttttc attgggctag gcatcttctt ctgtgtcagg tgccggcact gaaggcgacc
     6421 tgcggatccc acgtcactat tgtatactct atattatact ctatgttata ctctgtaatc
     6481 ctactcaata aacgtgtcac gcctgtgaaa ccgtactaag tctcccgtgt cttcttatca
     6541 ccatcaggtg acatcctcgc ccaggctgtc aatcatgccg gtatcgattc cagtagcacc
     6601 ggccccacgc tgacaaccca ctcttgcagc gttagcagcg cccctcttaa caagccgacc
     6661 cccaccagcg tcgcggttac taacactcct ctccccgacc tgcagcccaa gcttggcgta
     6721 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6781 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6841 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6901 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6961 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7021 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7081 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7141 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7201 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7261 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7321 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7381 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7441 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7501 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7561 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7621 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7681 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7741 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7801 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7861 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7921 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7981 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8041 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8101 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8161 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8221 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8281 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8341 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8401 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8461 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8521 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8581 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8641 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8701 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8761 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8821 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8881 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8941 ctttcgtc
//
