LOCUS       sequence-358095-         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses copGFP and puromycin resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Wang B, Wang Z, Wang D, Zhang B, Ong SG, Li M, Yu W, Wang Y
  TITLE     krCRISPR: an easy and efficient strategy for generating conditional 
            knockout of essential genes in cells.
  JOURNAL   J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. 
            eCollection 2019.
  PUBMED    31049076
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2019-04-24"; volume: "13:35. doi"; pages: " 
            10.1186/s13036-019-0150-y. eCollection 2019"
COMMENT     Sequence Label: rescue plasmid
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(245..265)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     protein_bind    322..340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        422..736
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    430..448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    466..484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    501..519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    537..555
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    573..591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    608..626
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    644..662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     709..733
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat
      121 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg
      181 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgaacta gatgatccgg
      241 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      301 atgcaaagca tgcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
      361 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      421 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      481 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      541 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      601 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      661 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      721 gaaccgtcag atcgcctgga gaattcgagc tcggtacccg gggatcctct agtcagctga
      781 cgcgtgctag cgcggccgca tcgataagct tgtcgacgat atctctagag cgatcgctga
      841 ccggtgaggg cagaggaagt cttctaacat gcggtgacgt ggnggagaat cccggccctt
      901 ccggaatg
//
