LOCUS       sequence-358112-       10866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dendra2-based translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Lee S, Park D, Lim C, Kim JI, Min KT
  TITLE     mtIF3 is locally translated in axons and regulates mitochondrial 
            translation for axonal growth.
  JOURNAL   BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1186/s12915-021-01215-w.
  PUBMED    34996455
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-01-7"; volume: "20(1):12. doi"; pages: " 
            10.1186/s12915-021-01215-w"
COMMENT     Sequence Label: pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)
FEATURES             Location/Qualifiers
     source          1..10866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2055..2747
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             6782..6799
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6822..6839)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6828..7052
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7098..7526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7185..7204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7395..7416
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7535..7555)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7540..7869
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7720..7855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7782..7801
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7917..7964
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7983..8381
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8539..8660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8576..8595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8630..8649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8709..8725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8709..8725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8722..8744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8733..8749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8757..8763,8764..8781,8782..8787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8802..8823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8940..8957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9111..9699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9191..9210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9870..10661,10662..10730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10493..10512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10731..10835)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ggaagccgac gcggtgctcc ggcggagtcc aggtacggac ctgctagttc ttcctgctcg
      961 cctaccccgg cccagggtct cactatttat ctctggctgt cctgaaacgc actcggtaga
     1021 ccagtctgac ctcaaaccta tagaaatcct cctgcctcta cctccgaagt gctgggatta
     1081 aaggcgtgca ccgctcgccg tgtcaggact tttgaaattc cttccggaat tgaaggggta
     1141 ccatggctgt tcttctgatg aggttgatgc tacaaactac aaagcttgac cataacttaa
     1201 ttggaagatg tttgcaaaga cacgctgtga agccagatcc agcacagctg tccctgtctg
     1261 cgtccacccc aaagctgttg tacctgactt ctgcaaaagg ttttagtact gctggagacc
     1321 cacagggtga gaggaaacag aaaagaaggg atgctttctc taacaccggc cggaagatta
     1381 gtgagcggat tatccgcgtc ctggatgaga agggcatgga cctggggatg atgcatcgag
     1441 cagatgtgat cagactcatg aacaagcagg acctgaggct ggtgcagagg aacaccagct
     1501 cagagccacc ggagtaccag cttatgaccg gagagcagat ccaccaggag cggctaaagc
     1561 tccgggaaca ggaaaaggcc aaacccaaaa ctggaccaac tatgacgaaa gagctcgttt
     1621 tctcttcaaa tattggacag cacgatttag acaccaagag taaacagatc cagcagtgga
     1681 tagagaagaa ataccacgtt caagtcacga taaagaggag gaaagatgcg gagcagtcag
     1741 aggaggaaac ggaggagata tttaaccaga ttctccagac tatgcctgac atagcaacct
     1801 tctcaagcag accaaaagcc atcagaggag ggactgcttc catgtgtgtt tttcgtcact
     1861 tgagcaagaa agaagaaaag gcgtacagag aatcccagga gagccagaga agagacactt
     1921 tgagcaaaga cgacgacgga aacagcaaag agtcggatgt tgtgtgtcag accggtgaag
     1981 cggcggcgaa agaagcggcg gcgaaagaag cggcggcgaa aatgctgtgc tgtatgctgt
     2041 gctgtgtcgc caccatgaac accccgggaa ttaacctgat caaggaggac atgcgcgtga
     2101 aggtgcacat ggagggcaac gtgaacggcc acgccttcgt gatcgagggc gagggcaagg
     2161 gcaagcccta cgagggcacc cagaccgcca acctgaccgt gaaggagggc gcccccctgc
     2221 ccttcagcta cgacatcctg accaccgccg tgcactacgg caaccgggtg ttcaccaagt
     2281 accccgagga catccccgac tacttcaagc agagcttccc cgagggctac agctgggagc
     2341 gcaccatgac cttcgaggac aagggcatct gcaccatccg cagcgacatc agcctggagg
     2401 gcgactgctt cttccagaac gtgcgcttca agggcaccaa cttccccccc aacggccccg
     2461 tgatgcagaa gaagaccctg aagtgggagc ccagcaccga gaagctgcac gtgcgcgacg
     2521 gcctgctggt gggcaacatc aacatggccc tgctgctgga gggcggcggc cactacctgt
     2581 gcgacttcaa gaccacctac aaggccaaga aggtggtgca gctgcccgac gcccacttcg
     2641 tggaccaccg catcgagatc ctgggcaacg acagcgacta caacaaggtg aagctgtacg
     2701 agcacgccgt ggcccgctac agccccctgc ccagccaggt gtggtaagcg gccgccttta
     2761 ataggaaaag tgcaggccag gagcctctgc tggccgccct gaagtcttcc cgcctagcag
     2821 acagtgagta aggcggctgg cgggggcagc tgggcctctg ggctgctgct ggactctgcg
     2881 ctggcgttag gcaaggagta gtcgggctct tgtttcctct taggagggaa gtacccactg
     2941 aggcgagagg ccaagctgcg tctctcccga ggggctctta ccttcaggag tgaggaagac
     3001 agacaactga gttgcccatt acacaacggg gatccactcc aacagatgag cccctgatgg
     3061 gagtcacacc ctcagcaggc acagacacgc aggatggggt atgcggatgg acagacgcca
     3121 agcaggcctc acagccgagt gctggcccca gcagctgcca cttactttga gcttcatcct
     3181 cttcttgtcg ggatcgtgca cgacactgtg cctcatgaga ctctgctgca gtgcaaggga
     3241 aagaaggacg cttgacctgg gcctgcagac gtggctcagt gcttacagca ttcgctgctc
     3301 gaggaggaca cgggtttggt tcccagcacc cgtactccag ttccagtaaa aactcttggt
     3361 gtctgctaac cttaagatgc agtggtccag tgtgcaataa taaactacca gctgcctttc
     3421 tgttgtgtag tgaatctgac cttcacctga ctccctaacc agagccaact ccttggcaga
     3481 gcgagcgact gtcagcctgc tgacacgcat gggatttact tcttcccggt cctcctgaga
     3541 agggaaagcc ggctatgctt gaacatccag gctcatgctg gggtaactga atctacctca
     3601 gctgcgtccc taagtgccag ggggaaatct gacctctgcc ctgctggcta tgctgacagt
     3661 gctgaccacg ggacctttgc tgtgggctgg tctgggttat gttcagtgac tttgtaatcc
     3721 acagtaagag ctctttagaa accagctagg cttaggcaaa ctgttcagtt ccctactagg
     3781 aaccatgttc ccacgcgatc catgaatcat gccaagattc cagagtctag aggctgtgca
     3841 tttgtgctac ccacctcctc tcccagaccc taggcgacgg tcagaagctc ctaatcacct
     3901 cagaacatcc tctaagtagg aatacaggcc ccgggctctc ccactcagca tggctagaat
     3961 cccccaggag tggttctcaa cctgctactc atgactcctc tggggtcata taccagatcg
     4021 cctgaatatc attatttaca atccatacca gtagcaaaat tatagttatg aagtagcaac
     4081 taaataatat tatggttggg ggtccccaca acatgaggaa ctgttattgg gtcccagtat
     4141 taggaaggtt gagaaccact gctctagagg tcctaaatag gcctgtttag cctcccaacc
     4201 ccagggctcc tgcatttcca agttctgcag gctggacatg aacacaccag cctggagcgg
     4261 gagacactca ctttcattgc gaatgtcttg ccacagccag cgtgctcaca cacaaatggg
     4321 cgcttttcct cgtggaagga gagaatgtgg ctctgcaggt tgaacacggt tgtgtaggtt
     4381 cttccgcagc cttgccgcgg acagcggtac acatcccttt ccggggcgtg agtcttcatg
     4441 tgctgcttaa ggtaatctct gcgcttgaac atcctctgac atacattgca ggttatgtcc
     4501 tctgggagga gaaacacaca cgtgtaacca gcgtcccatg agtaacccgt ctggtcctat
     4561 gactacacac aatctgggga cttcacatgg gaaatgtcag cttgtctcac tgctatgctg
     4621 gtttcaaatt cctcaagttc actcatggct cccaacatcc tgagtcctcg cctgtcaagg
     4681 agcaggcgtc ctcggtgtga taactgagca cgaggaagta tgccctcata gcatctacct
     4741 ttaccctctg acacagtagc atctgctaag tcctgcaggg cctcacgaac acccgggcac
     4801 agccgcacca gctctgaggc ctgcagggct ccctcgactg tcacagccca ggaagccagc
     4861 actaccctga actcgatgca gagccccctt cctttacagc gcaagtgggt atctcactct
     4921 ctcagagtac accatgcact ccccagagct cagagcacac acaccatggg ctccccgggg
     4981 ttcagagcac accgggctct ccccagggcc gatgcgcttc cacagctcct gcctacctta
     5041 cctttatggg cttctctcat gtgtttcagg agctctgtcc acgtttttcc catgaaagaa
     5101 catccctttt gacacaggta gcctgttcaa aacaaaaaca aaacaagtta cccacagcct
     5161 gttgaatgcc cagggcaatc cctaatgaca gccaagaaag cccgtgccaa gtgcactccc
     5221 ctctctgaag tgtgctgctc tgctgggtgc aatgcagcgg gctccagtgg cccctgctct
     5281 gaaagctccc atcagccaga cttttgcaca cttgagtggc acataaccag gtagcgtagg
     5341 aacagtatag caccagtgga cgtggcaaag aggatagcgg caaaacacac agcacagtgt
     5401 ctcttggcat aagaggcaaa cactcaaata acacaacagg cgcttgcttg tcacagaact
     5461 ttgtctactc agcgtgacta cagaatagac ccagcctacc ctgccggtgg catgttcctc
     5521 gcggccggca cagtccccat ctacgtaccc tcgtgaactt tcccatgccg tttcagcctg
     5581 ctgggcgagg caaagtgctt cccgcatccc tcgtgggtac acctaggaca atagcaggca
     5641 aagcatcagc caggcccttt ccatggacaa ttctaagatt ccctcttctc ccacaccaaa
     5701 gcccatgtct tcccacacca cacctgtcat ccaaaacaaa gagtaagtac agtcccgaat
     5761 atggacatag tggcttggcc agcttgggag caaaacaagc catcctgaga aaaagtcaca
     5821 tcttttgttt gtgttatcaa actaaaagtt ctctgaattc tggaaatgtg gctacagaag
     5881 cgacatcaag aggctgtctg actcggtttg cactggacca gacacagagc tgttgtctgg
     5941 ggtctgccca atatgctgca tacggtcaga tgagaacaca gcacagaggc tggggaccag
     6001 caccagaaac acacgagaat gtacagcagt cggtgcagag ctccaggcac tggcagcaca
     6061 aacaggccac agggctgctg gtaagacctc ggtcactgta gctgcaccag tgcaaagagc
     6121 agcctatggg agccaggggc aaggccccat cacccggact gtgcagggag acactgccct
     6181 aggagggagg caggcaggta acctggcacc cctgcccacc tcctctttgt ccctcttgag
     6241 tactcaggaa tccctcaaaa caagcagggc ctcattactc ctgttctata gtaagaaaaa
     6301 aaaaagccct catgacacag agccttcctc gggatcctga tcttagcgtt ctactctgaa
     6361 tcccagagcg ctggcaaagc accaacctga agagcggctc gctggtgtgc tggcactgat
     6421 gggttctcag ctgctggtgc ttcttaaagg ccttcttgca accctcataa ctgcactgtt
     6481 taaaaaaaat aaatgcacgg tttagtacag gcaggggatc atcagttgct gaagtccttt
     6541 aaaaggcagg agagattgct caatggttag ggacacttgc ttctctggtc ccaggtttgg
     6601 actgcagcac ccacgtggtg cctcacaacc atctgactcc agttccacag gacctcacac
     6661 cctcttctga cctccacagg caccgagcac gcatatggta tatagataca tatgcaggca
     6721 aaacgcttgc acataaaacc ttttttaact aaacaataaa gtctctctgg atctgaccgg
     6781 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
     6841 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6901 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6961 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     7021 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     7081 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     7141 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     7201 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7261 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7321 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     7381 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7441 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7501 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     7561 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7621 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7681 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7741 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7801 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7861 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     7921 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7981 ccatggccaa gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa
     8041 tcaacagcat ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg
     8101 gccgcatctt cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg
     8161 tggtgctggg cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg
     8221 gaaatgagaa caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc
     8281 tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga
     8341 ttcgtgaatt gctgccctct ggttatgtgt gggagggcta agcacttcgt ggccgaggag
     8401 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8461 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8521 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8581 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8641 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8701 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8761 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8821 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8881 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8941 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9001 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9061 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9121 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9181 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9241 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9301 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9361 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9421 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9481 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9541 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9601 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9661 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     9961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    10501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    10561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    10621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    10681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    10741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    10801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    10861 gacgtc
//
