LOCUS       sequence-358114-        9987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dendra2-based translation reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Lee S, Park D, Lim C, Kim JI, Min KT
  TITLE     mtIF3 is locally translated in axons and regulates mitochondrial 
            translation for axonal growth.
  JOURNAL   BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1186/s12915-021-01215-w.
  PUBMED    34996455
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-01-7"; volume: "20(1):12. doi"; pages: " 
            10.1186/s12915-021-01215-w"
COMMENT     Sequence Label: pcDNA6-5'UTR(mtIF3)-Dendra2-3'UTR(mtIF3)
FEATURES             Location/Qualifiers
     source          1..9987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1176..1868
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             5903..5920
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5943..5960)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5949..6173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6219..6647
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6306..6325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6516..6537
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6656..6676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6661..6990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6841..6976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6903..6922
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7038..7085
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7104..7502
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7660..7781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7697..7716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7751..7770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7830..7846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7830..7846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7843..7865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7854..7870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7878..7884,7885..7902,7903..7908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7923..7944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8061..8078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8232..8820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8312..8331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8991..9782,9783..9851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9614..9633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9852..9956)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ggaagccgac gcggtgctcc ggcggagtcc aggtacggac ctgctagttc ttcctgctcg
      961 cctaccccgg cccagggtct cactatttat ctctggctgt cctgaaacgc actcggtaga
     1021 ccagtctgac ctcaaaccta tagaaatcct cctgcctcta cctccgaagt gctgggatta
     1081 aaggcgtgca ccgctcgccg tgtcaggact tttgaaattc cttccggaat tgaaggggta
     1141 ccatgctgtg ctgtatgctg tgctgtgtcg ccaccatgaa caccccggga attaacctga
     1201 tcaaggagga catgcgcgtg aaggtgcaca tggagggcaa cgtgaacggc cacgccttcg
     1261 tgatcgaggg cgagggcaag ggcaagccct acgagggcac ccagaccgcc aacctgaccg
     1321 tgaaggaggg cgcccccctg cccttcagct acgacatcct gaccaccgcc gtgcactacg
     1381 gcaaccgggt gttcaccaag taccccgagg acatccccga ctacttcaag cagagcttcc
     1441 ccgagggcta cagctgggag cgcaccatga ccttcgagga caagggcatc tgcaccatcc
     1501 gcagcgacat cagcctggag ggcgactgct tcttccagaa cgtgcgcttc aagggcacca
     1561 acttcccccc caacggcccc gtgatgcaga agaagaccct gaagtgggag cccagcaccg
     1621 agaagctgca cgtgcgcgac ggcctgctgg tgggcaacat caacatggcc ctgctgctgg
     1681 agggcggcgg ccactacctg tgcgacttca agaccaccta caaggccaag aaggtggtgc
     1741 agctgcccga cgcccacttc gtggaccacc gcatcgagat cctgggcaac gacagcgact
     1801 acaacaaggt gaagctgtac gagcacgccg tggcccgcta cagccccctg cccagccagg
     1861 tgtggtaagc ggccgccttt aataggaaaa gtgcaggcca ggagcctctg ctggccgccc
     1921 tgaagtcttc ccgcctagca gacagtgagt aaggcggctg gcgggggcag ctgggcctct
     1981 gggctgctgc tggactctgc gctggcgtta ggcaaggagt agtcgggctc ttgtttcctc
     2041 ttaggaggga agtacccact gaggcgagag gccaagctgc gtctctcccg aggggctctt
     2101 accttcagga gtgaggaaga cagacaactg agttgcccat tacacaacgg ggatccactc
     2161 caacagatga gcccctgatg ggagtcacac cctcagcagg cacagacacg caggatgggg
     2221 tatgcggatg gacagacgcc aagcaggcct cacagccgag tgctggcccc agcagctgcc
     2281 acttactttg agcttcatcc tcttcttgtc gggatcgtgc acgacactgt gcctcatgag
     2341 actctgctgc agtgcaaggg aaagaaggac gcttgacctg ggcctgcaga cgtggctcag
     2401 tgcttacagc attcgctgct cgaggaggac acgggtttgg ttcccagcac ccgtactcca
     2461 gttccagtaa aaactcttgg tgtctgctaa ccttaagatg cagtggtcca gtgtgcaata
     2521 ataaactacc agctgccttt ctgttgtgta gtgaatctga ccttcacctg actccctaac
     2581 cagagccaac tccttggcag agcgagcgac tgtcagcctg ctgacacgca tgggatttac
     2641 ttcttcccgg tcctcctgag aagggaaagc cggctatgct tgaacatcca ggctcatgct
     2701 ggggtaactg aatctacctc agctgcgtcc ctaagtgcca gggggaaatc tgacctctgc
     2761 cctgctggct atgctgacag tgctgaccac gggacctttg ctgtgggctg gtctgggtta
     2821 tgttcagtga ctttgtaatc cacagtaaga gctctttaga aaccagctag gcttaggcaa
     2881 actgttcagt tccctactag gaaccatgtt cccacgcgat ccatgaatca tgccaagatt
     2941 ccagagtcta gaggctgtgc atttgtgcta cccacctcct ctcccagacc ctaggcgacg
     3001 gtcagaagct cctaatcacc tcagaacatc ctctaagtag gaatacaggc cccgggctct
     3061 cccactcagc atggctagaa tcccccagga gtggttctca acctgctact catgactcct
     3121 ctggggtcat ataccagatc gcctgaatat cattatttac aatccatacc agtagcaaaa
     3181 ttatagttat gaagtagcaa ctaaataata ttatggttgg gggtccccac aacatgagga
     3241 actgttattg ggtcccagta ttaggaaggt tgagaaccac tgctctagag gtcctaaata
     3301 ggcctgttta gcctcccaac cccagggctc ctgcatttcc aagttctgca ggctggacat
     3361 gaacacacca gcctggagcg ggagacactc actttcattg cgaatgtctt gccacagcca
     3421 gcgtgctcac acacaaatgg gcgcttttcc tcgtggaagg agagaatgtg gctctgcagg
     3481 ttgaacacgg ttgtgtaggt tcttccgcag ccttgccgcg gacagcggta cacatccctt
     3541 tccggggcgt gagtcttcat gtgctgctta aggtaatctc tgcgcttgaa catcctctga
     3601 catacattgc aggttatgtc ctctgggagg agaaacacac acgtgtaacc agcgtcccat
     3661 gagtaacccg tctggtccta tgactacaca caatctgggg acttcacatg ggaaatgtca
     3721 gcttgtctca ctgctatgct ggtttcaaat tcctcaagtt cactcatggc tcccaacatc
     3781 ctgagtcctc gcctgtcaag gagcaggcgt cctcggtgtg ataactgagc acgaggaagt
     3841 atgccctcat agcatctacc tttaccctct gacacagtag catctgctaa gtcctgcagg
     3901 gcctcacgaa cacccgggca cagccgcacc agctctgagg cctgcagggc tccctcgact
     3961 gtcacagccc aggaagccag cactaccctg aactcgatgc agagccccct tcctttacag
     4021 cgcaagtggg tatctcactc tctcagagta caccatgcac tccccagagc tcagagcaca
     4081 cacaccatgg gctccccggg gttcagagca caccgggctc tccccagggc cgatgcgctt
     4141 ccacagctcc tgcctacctt acctttatgg gcttctctca tgtgtttcag gagctctgtc
     4201 cacgtttttc ccatgaaaga acatcccttt tgacacaggt agcctgttca aaacaaaaac
     4261 aaaacaagtt acccacagcc tgttgaatgc ccagggcaat ccctaatgac agccaagaaa
     4321 gcccgtgcca agtgcactcc cctctctgaa gtgtgctgct ctgctgggtg caatgcagcg
     4381 ggctccagtg gcccctgctc tgaaagctcc catcagccag acttttgcac acttgagtgg
     4441 cacataacca ggtagcgtag gaacagtata gcaccagtgg acgtggcaaa gaggatagcg
     4501 gcaaaacaca cagcacagtg tctcttggca taagaggcaa acactcaaat aacacaacag
     4561 gcgcttgctt gtcacagaac tttgtctact cagcgtgact acagaataga cccagcctac
     4621 cctgccggtg gcatgttcct cgcggccggc acagtcccca tctacgtacc ctcgtgaact
     4681 ttcccatgcc gtttcagcct gctgggcgag gcaaagtgct tcccgcatcc ctcgtgggta
     4741 cacctaggac aatagcaggc aaagcatcag ccaggccctt tccatggaca attctaagat
     4801 tccctcttct cccacaccaa agcccatgtc ttcccacacc acacctgtca tccaaaacaa
     4861 agagtaagta cagtcccgaa tatggacata gtggcttggc cagcttggga gcaaaacaag
     4921 ccatcctgag aaaaagtcac atcttttgtt tgtgttatca aactaaaagt tctctgaatt
     4981 ctggaaatgt ggctacagaa gcgacatcaa gaggctgtct gactcggttt gcactggacc
     5041 agacacagag ctgttgtctg gggtctgccc aatatgctgc atacggtcag atgagaacac
     5101 agcacagagg ctggggacca gcaccagaaa cacacgagaa tgtacagcag tcggtgcaga
     5161 gctccaggca ctggcagcac aaacaggcca cagggctgct ggtaagacct cggtcactgt
     5221 agctgcacca gtgcaaagag cagcctatgg gagccagggg caaggcccca tcacccggac
     5281 tgtgcaggga gacactgccc taggagggag gcaggcaggt aacctggcac ccctgcccac
     5341 ctcctctttg tccctcttga gtactcagga atccctcaaa acaagcaggg cctcattact
     5401 cctgttctat agtaagaaaa aaaaaagccc tcatgacaca gagccttcct cgggatcctg
     5461 atcttagcgt tctactctga atcccagagc gctggcaaag caccaacctg aagagcggct
     5521 cgctggtgtg ctggcactga tgggttctca gctgctggtg cttcttaaag gccttcttgc
     5581 aaccctcata actgcactgt ttaaaaaaaa taaatgcacg gtttagtaca ggcaggggat
     5641 catcagttgc tgaagtcctt taaaaggcag gagagattgc tcaatggtta gggacacttg
     5701 cttctctggt cccaggtttg gactgcagca cccacgtggt gcctcacaac catctgactc
     5761 cagttccaca ggacctcaca ccctcttctg acctccacag gcaccgagca cgcatatggt
     5821 atatagatac atatgcaggc aaaacgcttg cacataaaac cttttttaac taaacaataa
     5881 agtctctctg gatctgaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     5941 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6001 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6061 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6121 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6181 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6241 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6301 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6361 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6421 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6481 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6541 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6601 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6661 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6721 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6781 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6841 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6901 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6961 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7021 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     7081 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     7141 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     7201 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     7261 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     7321 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     7381 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     7441 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     7501 aagcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7561 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7621 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7681 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7741 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7801 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7861 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9961 tttccccgaa aagtgccacc tgacgtc
//
