LOCUS       sequence-358115-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 WT expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 WT
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..30
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             66..89
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 catcaacaag tttgtacaaa aaagcaggct ctttaaagga accaattcag tcgactggat
       61 ccatggacta caaagacgat gacgacaagg aatcaccaga ggagcctgga gcatccatgg
      121 atgagaacta ctttgtgaac tacactttca aagatcggtc acattcaggc cgtgtggctc
      181 aaggcatcat gaaactgtgt ctagaggagg agctctttgc tgatgtcacc atttcggtgg
      241 aaggccggga gtttcagctc catcggctgg tcctctcagc tcagagctgc ttcttccgat
      301 ccatgttcac ttccaacctg aaggaggccc acaaccgggt gattgtgctg caggatgtca
      361 gcgagtctgt tttccagctc ctggttgatt atatctacca tgggactgtg aaacttcgag
      421 ctgaggagtt gcaggaaatt tatgaggtgt cagacatgta tcagctgaca tctctctttg
      481 aggaatgctc tcggtttttg gcccgcacag tgcaagtggg aaactgcctt caggtgatgt
      541 ggctggcaga tcggcacagt gatcctgagc tctatacggc tgccaagcac tgtgccaaga
      601 cccacctggc ccagctgcag aatacagagg aatttctcca cttgccccac cgcttactca
      661 cagatatcat ctcggatgga gttccgtgtt ctcagaaccc aacagaggca atagaagcct
      721 ggatcaactt taataaagag gaaagagagg cttttgcaga gtcactcagg acaagcttga
      781 aggaaattgg ggagaatgtg cacatttacc tgattgggaa agagtcatct cgtacccact
      841 cgttggctgt gtccttgcac tgtgcagaag atgactccat cagtgtaagt ggccaaaaca
      901 gtttgtgcca ccagatcact gcggcctgca agcatggt
//
