LOCUS       sequence-358116-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 WT expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 WT
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    46..70
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggatcctta gtggtggtgg tggtggtgga ccggttcatt actaaaccac tttgtacaag
       61 aaagctgggt ctagatatct cgagtgcggc cgcgaattct taggccaaca caaactgcaa
      121 attggtaagc agcaccttaa tacctcttgt gacagttacg gctgaagtgg cagggtcaag
      181 cttgtaggtg ttggcatccc cctttttata tcggtcccgg aagacatasa tgctcccgtt
      241 acagctggca atcttccaga gggatggggc agagctccag gcctcaggaa ggcaaagccg
      301 ggtgaagctg tctagcaagg gattgtagca caccagggag tccccttcag ccwcgatgaa
      361 caccaratct ttatgcacag ctgcgtgcat gcggcctgsa aagggcagca catagggctt
      421 cacatggcat ttgtctgtct ctgtgtcaaa gcactggatg agtcgggaag gtttggtaaa
      481 gaagtccaga tcattctcct ccccccctag taagtagatg atcccgttga ggttggcacc
      541 agcagcccct gacacagcca cctctagctg agttgtctct gtccacacat tatcacctac
      601 gcgataataa atgactgcgt tggagagggt atcttgcagt gtcttgccac ccagtgaata
      661 tatggcatct ttcccgggca cagacaccag ggtgtgctgg agccggtccc gaggcaaagg
      721 agcacaccac tcccagtcaa cggtggcatt gttgcacttc cacatgcgcc gtgggatgga
      781 ccctcccacc acatacaagt ctccaccatg cttgcaggcc gcagtgatct ggtggcacaa
      841 actgttttgg ccacttacac tgatggagtc atcttctgca cagtgcaagg acacagccaa
      901 cgagtgggta cgagatgact ctttcccaat caggtaaatg tgcacattct cc
//
