LOCUS       sequence-358117-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 P311PP expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 P311PP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caacaagttt gtacaaaaaa gcaggctctt tanaggaacc aattcagtcg actggatcca
       61 tggactacaa agacgatgac gacaaggaat caccagagga gcctggagca tccatggatg
      121 agaactactt tgtgaactac actttcaaag atcggtcaca ttcaggccgt gtggctcaag
      181 gcatcatgaa actgtgtcta gaggaggagc tctttgctga tgtcaccatt tcggtggaag
      241 gccgggagtt tcagctccat cggctggtcc tctcagctca kagctgcttc ttccgatcca
      301 tgttcacttc caacctgaag gaggcccaca accgggtgat tgtgctgcag gatgtcagcg
      361 agtctgtttt ccagctcctg gttgattata tctaccatgg gactgtgaaa cttcgagctg
      421 aggagttgca ggaaatttat gaggtgtcag acatgtatca gctgacatct ctctttgagg
      481 aatgctctcg gtttttggcc cgcacagtgc aagtgggaaa ctgccttcag gtgatgtggc
      541 tggcagatcg gcacagtgat cctgagctct atacggctgc caagcactgt gccaagaccc
      601 acctggccca gctgcagaat acagaggaat ttctccactt gccccaccgc ttactcacag
      661 atatcatctc ggatggagtt ccgtgttctc agaacccaac agaggcaata gaagcctgga
      721 tcaactttaa taaagaggaa agagaggctt ttgcagagtc actcaggaca agcttgaagg
      781 aaattgggga gaatgtgcac atttacctga ttgggaaaga gtcatctcgt acccactcgt
      841 tggctgtgtc cttgcactgt gcagaagatg actccatcag tgtaagtggc caaaacagtt
      901 tgtgccacca gatcactgcg gcctgcaagc atggtgg
//
