LOCUS       sequence-358118-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 R313PRR expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 R313PRR
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             60..83
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caagtttgta caaaaaagca ggctctttaa aggaaccaat tcagtcgact ggatccatgg
       61 actacaaaga cgatgacgac aaggaatcac cagaggagcc tggagcatcc atggatgaga
      121 actactttgt gaactacact ttcaaagatc ggtcacattc aggccgtgtg gctcaaggca
      181 tcatgaaact gtgtctagag gaggagctct ttgctgatgt caccatttcg gtggaaggcc
      241 gggagtttca gctccatcgg ctggtcctct cagctcagag ctgcttcttc cgatccatgt
      301 tcacttccaa cctgaaggag gcccacaacc gggtgattgt gctgcaggat gtcagcgagt
      361 ctgttttcca gctcctggtt gattatatct accatgggac tgtgaaactt cgagctgagg
      421 agttgcagga aatttatgag gtgtcagaca tgtatcagct gacatctctc tttgaggaat
      481 gctctcggtt tttggcccgc acagtgcaag tgggaaactg ccttcaggtg atgtggctgg
      541 cagatcggca cagtgatcct gagctctata cggctgccaa gcactgtgcc aagacccacc
      601 tggcccagct gcagaataca gaggaatttc tccacttgcc ccaccgctta ctcacagata
      661 tcatctcgga tggagttccg tgttctcaga acccaacaga ggcaatagaa gcctggatca
      721 actttaataa agaggaaaga gaggcttttg cagagtcact caggacaagc ttgaaggaaa
      781 ttggggagaa tgtgcacatt tacctgattg ggaaagagtc atctcgtacc cactcgttgg
      841 ctgtgtcctt gcactgtgca gaagatgact ccatcagtgt aagtggccaa aacagtttgt
      901 gcc
//
