LOCUS       sequence-358120-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pcDNA 3.1 KBTBD4 WT
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..43
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     88..107
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        88..106
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             123..200
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
ORIGIN
        1 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
       61 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctta
      121 tggattacaa ggatgacgac gataagggag attacaagga tgacgacgat aagatcgatt
      181 acaaggatga cgacgataag ggatccatgg aatcaccaga ggagcctgga gcatccatgg
      241 atgagaacta ctttgtgaac tacactttca aagatcggtc acattcaggc cgtgtggctc
      301 aaggcatcat gaaactgtgt ctagaggagg agctctttgc tgatgtcacc atttcggtgg
      361 aaggccggga gtttcagctc catcggctgg tcctctcagc tcagagctgc ttcttccgat
      421 ccatgttcac ttccaacctg aaggaggccc acaaccgggt gattgtgctg caggatgtca
      481 gcgagtctgt tttccagctc ctggttgatt atatctacca tgggactgtg aaacttcgag
      541 ctgaggagtt gcaggaaatt tatgaggtgt cagacatgta tcagctgaca tctctctttg
      601 aggaatgctc tcggtttttg gcccgcacag tgcaagtggg aaactgcctt caggtgatgt
      661 ggctggcaga tcggcacagt gatcctgagc tctatacggc tgccaagcac tgtgccaaga
      721 cccacctggc ccagctgcag aatacagagg aatttctcca cttgccccac cgcttactca
      781 cagatatcat ctcggatgga gttccncgtt ctcagaacca
//
