LOCUS       sequence-358122-        1078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pcDNA 3.1 KBTBD4 P311PP
FEATURES             Location/Qualifiers
     source          1..1078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     82..101
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        82..100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..194
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
ORIGIN
        1 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
       61 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttatggatt
      121 acaaggatga cgacgataag ggagattaca aggatgacga cgataagatc gattacaagg
      181 atgacgacga taagggatcc atggaatcac cagaggagcc tggagcatcc atggatgaga
      241 actactttgt gaactacact ttcaaagatc ggtcacattc aggccgtgtg gctcaaggca
      301 tcatgaaact gtgtctagag gaggagctct ttgctgatgt caccatttcg gtggaaggcc
      361 gggagtttca gctccatcgg ctggtcctct cagctcagag ctgcttcttc cgatccatgt
      421 tcacttccaa cctgaaggag gcccacaacc gggtgattgt gctgcaggat gtcagcgagt
      481 ctgttttcca gctcctggtt gattatatct accatgggac tgtgaaactt cgagctgagg
      541 agttgcagga aatttatgag gtgtcagaca tgtatcagct gacatctctc tttgaggaat
      601 gctctcggtt tttggcccgc acagtgcaag tgggaaactg ccttcaggtg atgtggctgg
      661 cagatcggca cagtgatcct gagctctata cggctgccaa gcactgtgcc aagacccacc
      721 tggcccagct gcagaataca gaggaatttc tccacttgcc ccaccgctta ctcacagata
      781 tcatctcgga tggagttccg tgttctcaga acccaacaga ggcaatagaa gcctggatca
      841 actttaataa agaggaaaga gaggcttttg cagagtcact caggacaagc ttgaaggaaa
      901 ttggggagaa tgtgcacatt tacctgattg ggaaagagtc atctcgtacc cactcgttgg
      961 ctgtgtcctt gcactgtgca gaagatgact ccatcagtgt aagtggccaa aacagtttgt
     1021 gccaccagat cactgcggcc tgcaagcatg gtggagactt gtatgtggtg ggagggtc
//
