LOCUS       sequence-358126-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pcDNA 3.1 KBTBD4 R312RG
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             75..152
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
ORIGIN
        1 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tatggattac aaggatgacg acgataaggg agattacaag gatgacgacg
      121 ataagatcga ttacaaggat gacgacgata agggatccat ggaatcacca gaggagcctg
      181 gagcatccat ggatgagaac tactttgtga actacacttt caaagatcgg tcacattcag
      241 gccgtgtggc tcaaggcatc atgaaactgt gtctagagga ggagctcttt gctgatgtca
      301 ccatttcggt ggaaggccgg gagtttcagc tccatcggct ggtcctctca gctcagagct
      361 gcttcttccg atccatgttc acttccaacc tgaaggaggc ccacaaccgg gtgattgtgc
      421 tgcaggatgt cagcgagtct gttttccagc tcctggttga ttatatctac catgggactg
      481 tgaaacttcg agctgaggag ttgcaggaaa tttatgaggt gtcagacatg tatcagctga
      541 catctctctt tgaggaatgc tctcggtttt tggcccgcac agtgcaagtg ggaaactgcc
      601 ttcaggtgat gtggctggca gatcggcaca gtgatcctga gctctatacg gctgccaagc
      661 actgtgccaa gacccacctg gcccagctgc agaatacaga ggaatttctc cacttgcccc
      721 accgcttact cacagatatc atctcggatg gagttccgtg ttctcagaac ccaacagagg
      781 caatagaagc ctgsatcaac tttaataaag aggaaagaga ggcttttgca gagtcactca
      841 ggacaagctt gaaggaaatt ggggagaatg tgcacattta cctgattggg aaagagtcat
      901 ctcgtaccca ctcgttggct gtgtccttgc actgtgcaga agatgactcc atcagtgtaa
      961 gtggccaaaa cagtttgtgc caccagatca ctgcggcctg caagcatggt ggnnncttgt
     1021 atgtgnnngg agggtccatc ccacgggnac gcatgtgga
//
