LOCUS       sequence-358134-        9150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a targeting vector, to target the endogenous DAZL CPG island with 
            Snrp-GFP-polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R
  TITLE     Tracing Dynamic Changes of DNA Methylation at Single-Cell 
            Resolution.
  JOURNAL   Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
  PUBMED    26406378
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.08"; journalName: "Cell"; date: "2015-09-24- 
            24"; volume: "163"; issue: "1"; pages: "218-29"
COMMENT     Sequence Label: Dazl-CPG-TV
FEATURES             Location/Qualifiers
     source          1..9150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(1753..1755,1756..1758,1759..2472)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1753..1755,1756..1758,1759..2469)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1798..1819)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2059..2078)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2406..2427
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2562..2581)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2627..2682
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2681..2700)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    3043..3076
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3137..3636
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3172..3195)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3569..3588
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3676..4275
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3676..3695)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4832..4865
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5584..5603)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5585..5603)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5610..5627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5610..5626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5619..5641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5767..6195
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5854..5873)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6064..6085
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7202..7221
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(7350..7418,7419..8210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7568..7587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8381..8969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8870..8889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9123..9140
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 caggttatgc cctctcccca cttctctgtg ctacaccaga aaaggccgat ttttaaacct
      361 ccgcgtaagg aatcgcggcc tccggctggt cttgcactgc caccccgggg cctgccctcg
      421 actcaccatg atggcggcca gagttcgggt ggtaaaacct cgaaggtgga gcagaagcca
      481 cgcgacgggg cagctacgtg aggtggctgc ctagccggcc aacgggcaaa aaaagccgag
      541 gcggtggtgg aaggagagcc cacggccacg gagacagcgg agtcccgaag cgagcgtgct
      601 gagggtcgcc gagtcactga ggcgtgggct gcgcgccccg gggggatgac tgtgcgtggc
      661 tggcctcgtg acgtgctaca gccaataggc ggcagcgcta gggcctcgca aggggggcca
      721 ggggacccac gtcgcccacg gagtgcgtga ggggtgcgca tggagcatgc gcgacatcta
      781 cgtaagggtc tcggctgccg agcaggcccg agcctcactg acagacagat ggaccgactc
      841 ctgccactcc ctagcccccc caggaccgtg tccgaagccc ctgtgaaata agtcctgggc
      901 aggacggtgc tcagagttct gtcaggaaag catttattca agtcattacc tcaaaaatgg
      961 cacctcgaag gcatggcgtt atcccgtcag caaagcttgg cggccgaatt taaaatttac
     1021 aaaatgcccg cagaaataga aattaataac gggcggcacg actagagagc aggccttgcc
     1081 tggccgggcc ccttcacccc ctttttcagc cacattgaga aggttgagtc cgggtgcatg
     1141 tggaaggcgc acctcacgcc tcacgggccg gcccgtcagc catcaggact tagttttttt
     1201 cttccgtttt ttttctcccc cccccccccg ccccttcaaa taaatgcccg aattatccgg
     1261 tacaaaggac acagcagtga ccagaccaca cgagagaata tccctccctt cgtggaattt
     1321 aggttgcagt atgagtgttt ctcttgtagg ctttacagaa acaagttagg ccagctgaga
     1381 gaattctaga acattctcaa agccagagaa acggggccta cctacctaca gcagagccga
     1441 gctgtagggt gcttggcaat tgacgctcaa atttccgcag taggaatgct caagcattcc
     1501 ttttggtagc tgccttttgg caggacattc cggtcagagg gacagagacc cctgcattgc
     1561 ggcaaaaatg tgcgcatgtg cagccattgc ctgggacgca tgcgtaggga gccgcgcgac
     1621 aaacctgagc cattgcggca agactagcgc agagaggaga gggagccgga gatgccagac
     1681 gcttggttct gaggagtgat ttgcaacgca atggagcgag gaaggtcagc tgggcttgtg
     1741 gattctgcta gcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1801 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1861 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1921 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1981 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2041 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2101 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2161 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2221 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2281 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2341 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2401 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2461 ctgtacaagt aaacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     2521 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     2581 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     2641 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     2701 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     2761 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     2821 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     2881 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     2941 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     3001 tggagatccc ttaattaacg atcatattca ataaccctta atataacttc gtataatgta
     3061 tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct taggatctcg
     3121 acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     3181 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     3241 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     3301 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     3361 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     3421 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     3481 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     3541 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     3601 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct agatctcgag
     3661 cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     3721 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     3781 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     3841 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     3901 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     3961 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4021 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4081 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4141 gtgcccgcct tcctggagac ctccgcgccc cacaacctcc ccttctacga gcggctcggc
     4201 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4261 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     4321 ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct attaaacaat
     4381 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
     4441 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
     4501 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
     4561 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     4621 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt
     4681 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca
     4741 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat
     4801 cagaagctgg tcgagatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     4861 gttattaggt ccctcgaaga ggttcactag gcgcgcctgg agataacctt acggcagaac
     4921 cacctagtac ttataccgtc ctgactacct tttgtacaga tgataagtat tacagctttt
     4981 ttttttttta aagctggcaa aacagcttga ctgccactaa ccatgcagca atgatggaga
     5041 aaatagtatt aaaataataa ataaaaataa aacaaataag ggtagattgc acctatttga
     5101 aaggggggaa attccaaaat tctgaaacta aaattaagct cgaaactaca gcggtgaaga
     5161 tccgattctc cactggcctt cggtaggtgg ctacatggtg gtgtgaacag tctaacaaag
     5221 ggcctggact gtcctcattt tagtgtagag aagaaaaaaa cccactacag acagctggaa
     5281 tggttggtta atgagcagcg gagagatggc tctaaactct gtagctccag gccttcctgc
     5341 gttggctgga tggggaaagc tgaagcggtg cagcccgatc tggagctgag caccttgcag
     5401 cacaccggtg ttctggcaag gcatcatcct gaggaaaagc acggacgtga ggggacgggg
     5461 agcaccgtgc agactagagc aaacccaaaa ccatctaact ctcattggtg tttgaccttc
     5521 cattcctgcg ctctccaagt ttggcggcgg ccgctcgagc atgcatctag agggcccaat
     5581 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     5641 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5701 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5761 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5821 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5881 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5941 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6001 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6061 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6121 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6181 aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac
     6241 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
     6301 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
     6361 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     6421 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     6481 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     6541 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6601 ctatgactgg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6661 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     6721 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     6781 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     6841 ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     6901 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     6961 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     7021 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     7081 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     7141 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     7201 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     7261 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7321 gagttcttct gaattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9121 gcagcgagtc agtgagcgag gaagcggaag
//
