LOCUS       sequence-358140-        2601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ZeoR backbone for recombinant protein overexpression with Drosophila
            ExpreS2 Platform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Coker JA, Katis VL, Fairhead M, Schwenzer A, Clemmensen SB, Frandsen
            BU, de Jongh WA, Gileadi O, Burgess-Brown NA, Marsden BD, Midwood 
            KS, Yue WW
  TITLE     FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult 
            Recombinant Proteins in Drosophila S2 Cells.
  JOURNAL   Front Bioeng Biotechnol. 2022 May 5;10:871933. doi: 
            10.3389/fbioe.2022.871933. eCollection 2022.
  PUBMED    35600892
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2022-05-5"; volume: "10:871933. doi"; 
            pages: " 10.3389/fbioe.2022.871933. eCollection 2022"
COMMENT     Sequence Label: pExpreS2-1
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(141..729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(221..240)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        986..1004
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1008..1017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1014..1388
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1452..1573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1489..1508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          2286..2418
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     polyA_signal    join(2478..2601,1..6)
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
ORIGIN
        1 cgcgtgggac tcgatgctcg gcgccactca aaggcggtaa tacggttatc cacagaatca
       61 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      121 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      181 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      241 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      301 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      361 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      421 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      481 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
      541 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
      601 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
      661 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
      721 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
      781 tcacgttaag ggattttgca tgcgctaagc gggctttata aaacgggctg cgggaccagt
      841 tttcatatca ctaccgtttg agttcttgtg ctgtgtggat actcctcccg acaccgaatt
      901 aattcggatc tctgcaaggg attttggtca tgaacaataa aactgtctgc ttacataaac
      961 agtaatacaa ggggtgttca tagtataata cgactcacta taggagggcc accatggcca
     1021 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     1081 ggaccgaccg gctcgggttc agccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     1141 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     1201 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     1261 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     1321 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     1381 aggactgacc gacgccgacc aacaccgccg gtccgacggc ggcccacggg tcccaggggg
     1441 gtcgacctcg aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1561 tcaatgtatc ttatcatgtc ttcacgtaat aagtgtgcgg ctagcagtca actagtccaa
     1621 actcatcaat gtatcttatc atgtcttcac gtaataagtg tgcggctagc agtcaactac
     1681 tagtgaatgc cctactagaa gatgtgtgtt gcacaaaatg tccctggaat aaccaatttg
     1741 aagtgcagat agcagtaaac gtaagctaat atgaatatta tttaactgta atgttttaat
     1801 atcgctggac attactaata aacccactat aaacacatgt acatatgtat gttttggcat
     1861 acaatgagta gttggggaaa aaatgtgtaa aagcaccgtg accatcacag cataaagata
     1921 accagctgaa gtatcgaata tgagtaaccc ccaaattgaa tcacatgccg caactgatag
     1981 gacccatgga agtacactct tcatggcgat atacaagaca cacacaagca cgaacaccca
     2041 gttgcggagg aaattctccg taaatgaaaa cccaatcggc gaacaattca tacccatata
     2101 tggtaaaagt tttgaacgcg agcgaactta agagcgccgg agtataaata gaggcgcttc
     2161 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
     2221 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagaagctt ggtaccctcg
     2281 agctcgtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
     2341 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
     2401 ttgcctttct ctccacagga attctggatc ctctagaccg gtcatatgcg gccgcggatc
     2461 gatcgatatc tgactaaatc ttagtttgta ttgtcatgtt ttaatacaat atgttatgtt
     2521 taaatatgtt tttaataaat tttataaaat aatttcaact tttattgtaa caacattgtc
     2581 catttacaca ctcctttcaa g
//
