LOCUS       sequence-358142-        2864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G418R backbone for recombinant protein overexpression with 
            Drosophila ExpreS2 Platform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Coker JA, Katis VL, Fairhead M, Schwenzer A, Clemmensen SB, Frandsen
            BU, de Jongh WA, Gileadi O, Burgess-Brown NA, Marsden BD, Midwood 
            KS, Yue WW
  TITLE     FAS2FURIOUS: Moderate-Throughput Secreted Expression of Difficult 
            Recombinant Proteins in Drosophila S2 Cells.
  JOURNAL   Front Bioeng Biotechnol. 2022 May 5;10:871933. doi: 
            10.3389/fbioe.2022.871933. eCollection 2022.
  PUBMED    35600892
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2022-05-5"; volume: "10:871933. doi"; 
            pages: " 10.3389/fbioe.2022.871933. eCollection 2022"
COMMENT     Sequence Label: pExpreS2-2
FEATURES             Location/Qualifiers
     source          1..2864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        115..133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             143..958
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     polyA_signal    973..1094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1010..1029)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    1870..1999
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(2134..2722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2214..2233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtgtggata ctcctcccga caccgaatta attcggatct ctgcaaggga ttttggtcat
       61 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttcat agtataatac
      121 gactcactat aggagggcca ccatgagcca catccagcgc gaaaccagct gcagccgtcc
      181 gcgcctgaac agcaacatgg atgccgatct gtacggctac aaatgggccc gcgataacgt
      241 gggccagagc ggcgctacca tctaccgcct gtacggcaaa ccggatgccc cggaactgtt
      301 cctgaaacac ggcaaaggca gcgtggccaa cgatgtgacc gatgaaatgg tgcgcctgaa
      361 ctggctgacc gagttcatgc cgctgccgac catcaaacac ttcatccgca ccccggatga
      421 tgcctggctg ctgaccaccg ccattccggg caaaaccgcc ttccaggtgc tggaagaata
      481 cccggatagc ggcgaaaaca tcgtggatgc cctggccgtg ttcctgcgcc gcctgcacag
      541 catcccggtg tgcaactgcc cgttcaacag cgatcgcgtg ttccgcctgg ctcaggccca
      601 gagccgcatg aacaacggcc tggtggatgc cagcgatttc gatgatgaac gcaacggctg
      661 gccggtggaa caggtgtgga aagagatgca caaactgctg ccgttcagcc cggattccgt
      721 ggtgacccac ggcgatttca gcctggataa cctgatcttc gatgagggca aactgatcgg
      781 ctgcatcgat gtgggccgcg tgggcattgc cgatcgctac caggatctgg ccatcctgtg
      841 gaactgcctg ggcgagttca gcccgagcct gcagaaacgc ctgttccaga agtacggcat
      901 cgataacccg gatatgaaca aactgcagtt ccacctgatg ctggatgagt tcttctaata
      961 agtcgacctc gaaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     1021 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     1081 atcaatgtat cttatcatgt cttcacgtaa taagtgtgcg gctagcagtc aactagtcca
     1141 aactcatcaa tgtatcttat catgtcttca cgtaataagt gtgcggctag cagtcaacta
     1201 ctagtgaatg ccctactaga agatgtgtgt tgcacaaaat gtccctggaa taaccaattt
     1261 gaagtgcaga tagcagtaaa cgtaagctaa tatgaatatt atttaactgt aatgttttaa
     1321 tatcgctgga cattactaat aaacccacta taaacacatg tacatatgta tgttttggca
     1381 tacaatgagt agttggggaa aaaatgtgta aaagcaccgt gaccatcaca gcataaagat
     1441 aaccagctga agtatcgaat atgagtaacc cccaaattga atcacatgcc gcaactgata
     1501 ggacccatgg aagtacactc ttcatggcga tatacaagac acacacaagc acgaacaccc
     1561 agttgcggag gaaattctcc gtaaatgaaa acccaatcgg cgaacaattc atacccatat
     1621 atggtaaaag ttttgaacgc gagcgaactt aagagcgccg gagtataaat agaggcgctt
     1681 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc
     1741 taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagaagct tggtaccctc
     1801 gagctcagct gaattctgga tcctctagac cggtcatatg cggccgcgga tcgatcgata
     1861 tctgactaaa tcttagtttg tattgtcatg ttttaataca atatgttatg tttaaatatg
     1921 tttttaataa attttataaa ataatttcaa cttttattgt aacaacattg tccatttaca
     1981 cactcctttc aagcgcgtgg gactcgatgc tcggcgccac tcaaaggcgg taatacggtt
     2041 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2101 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2161 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2221 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2281 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2341 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2401 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2461 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2521 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2581 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtaggtcttg
     2641 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2701 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2761 gtggaacgaa aactcacgtt aagggatttt gcatgcgcta agcgggcttt ataaaacggg
     2821 ctgcgggacc agttttcata tcactaccgt ttgagttctt gtgc
//
