LOCUS       sequence-358151-        1911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the GABA membrane transporter Gat1 R69K mutant fused to 
            HA, driven by the Ef1a promoter, in a Cre-dependent fashion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Melani R, Tritsch NX
  TITLE     Inhibitory co-transmission from midbrain dopamine neurons relies on 
            presynaptic GABA uptake.
  JOURNAL   Cell Rep. 2022 Apr 19;39(3):110716. doi: 
            10.1016/j.celrep.2022.110716.
  PUBMED    35443174
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-04-19"; volume: "39(3):110716. doi"; pages: " 
            10.1016/j.celrep.2022.110716"
COMMENT     Sequence Label: pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
FEATURES             Location/Qualifiers
     source          1..1911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1811..1900
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gctagccacc atggcgactg acaacagcaa ggtggctgat gggcagatct ctactgaggt
       61 cagcgaggcc cctgtggcca gcgacaagcc caaaaccctg gtagtcaagg tgcagaagaa
      121 ggccggggac ctccctgacc gggacacatg gaagggacgc ttcgacttcc tcatgtcctg
      181 cgtgggctat gccatcggcc tgggcaatgt gtggaagttc ccttacctct gtgggaaaaa
      241 cggtggcggg gccttcctaa tcccatattt cctgacgctc atctttgcgg gtgttcctct
      301 cttccttttg gagtgctccc taggccagta cacctccatt gggggcctgg gcgtatggaa
      361 gctggcgccc atgttcaagg gtgtgggcct cgcggcagct gtgctgtcct tctggctgaa
      421 catctactac atcgtcatca tctcctgggc catctactac ctgtacaact ccttcaccac
      481 gaccctgcca tggaaacagt gtgacaaccc gtggaacact gaccgctgct tctccaacta
      541 cagcctggtc aataccacca acatgaccag cgccgtggtg gagttctggg agcgcaacat
      601 gcaccagatg acagatggac tggacaagcc aggacagatc cgctggcctc tggccatcac
      661 actggccatt gcctgggtgc tcgtgtattt ctgcatctgg aagggtgttg gttggactgg
      721 aaaggtggtc tacttctcag ccacgtaccc ctacatcatg cttatcatcc tgttcttccg
      781 tggagtgacg cttcccgggg ccaaggaggg gatcctcttc tacatcacac ccaacttccg
      841 aaagctgtct gattctgagg tgtggcttga cgccgccacc cagatcttct tctcctacgg
      901 gctgggcctg gggtccctga ttgctctggg aagctacaac tctttccaca acaatgtgta
      961 cagggactcc atcatcgttt gctgcatcaa ctcctgcacc agcatgtttg ccggattcgt
     1021 catcttctcc atcgtgggct tcatggctca tgtcaccaag aggtccatag ctgatgtggc
     1081 agcctcaggc ccggggctgg cattcttggc gtaccctgag gctgtgacac agctacccat
     1141 ctctcccctc tgggctatcc tcttcttctc catgctgctg atgctgggca ttgacagcca
     1201 gttctgtacc gtggagggct tcatcactgc cctggtggac gagtacccca gacttctccg
     1261 caatcgccgt gaactcttca ttgctgccgt gtgcatcgtg tcctacctga ttggcctgtc
     1321 taacatcacc cagggtggca tttatgtctt caaactgttt gattattact ctgccagcgg
     1381 catgagcttg ctgttcctgg ttttcttcga gtgtgtctcc atttcctggt tttatggtgt
     1441 caaccggttc tatgacaaca tccaggagat ggttggctcc aggccctgca tctggtggaa
     1501 gctgtgctgg tcctttttca cacccatcat tgtggcgggc gtgtttctct tcagtgctgt
     1561 gcagatgaca ccactcacca tgggaagcta tgttttcccc aagtggggcc agggcgtggg
     1621 ctggctcatg gctctgtcct ccatggtgct catccccggg tacatggctt acatgttcct
     1681 caccctgaag ggctccctga agcagcgtct ccaggtcatg attcagccca gtgaagatat
     1741 tgtgcgccct gagaatggcc ctgagcagcc gcaggctggc agctcagcca gcaaggaggc
     1801 ctacatcggc tacccatacg atgttccaga ttacgctggc tatccctatg acgtcccgga
     1861 ctatgcagga tcctatccat atgacgttcc agattacgct tagggcgcgc c
//
