LOCUS       sequence-358163-        6122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell body-targeted jGCaMP8f, under synapsin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Shemesh OA, Linghu C, Piatkevich KD, Goodwin D, Celiker OT, Gritton 
            HJ, Romano MF, Gao R, Yu CJ, Tseng HA, Bensussen S, Narayan S, Yang 
            CT, Freifeld L, Siciliano CA, Gupta I, Wang J, Pak N, Yoon YG, 
            Ullmann JFP, Guner-Ataman B, Noamany H, Sheinkopf ZR, Park WM, Asano
            S, Keating AE, Trimmer JS, Reimer J, Tolias AS, Bear MF, Tye KM, Han
            X, Ahrens MB, Boyden ES
  TITLE     Precision Calcium Imaging of Dense Neural Populations via a 
            Cell-Body-Targeted Calcium Indicator.
  JOURNAL   Neuron. 2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029.
  PUBMED    32592656
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 26. pii: S0896-6273(20)30398-6. doi: 
            10.1016/j.neuron.2020.05.029."
COMMENT     Sequence Label: pAAV-Syn-Soma-jGCaMP8f
FEATURES             Location/Qualifiers
     source          1..6122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   58..187
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     polyA_signal    243..291
                     /note="synthetic polyadenylation signal"
     misc_feature    305..396
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     345..364
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        432..879
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             897..914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1196..1217
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1323..1344)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1584..1603)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2526..3114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2579..2599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2997..3008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3147..3164)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3153..3360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3369..3498
                     /label=AAV2 ITR
     rep_origin      3607..4062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3694..3713)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3904..3925
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4129..4148)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4248..4270
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4308..4326)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4394..4498
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4499..4567,4568..5359)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4717..4736)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5530..6118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6019..6038
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcct
       61 gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc gacctttggt
      121 cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc catcactagg
      181 ggttcctgcg gccgcacgcg ttctagaaat attaaggtac gggaggtact tggagcggcc
      241 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
      301 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
      361 tccccagtgc aagtgcaggt gccagaacat ttctctattc tagactcgag ctgcagaggg
      421 ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg gggtgcctac
      481 ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca aattgcgcat
      541 cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc actgccagct
      601 tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc
      661 actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc cggccacctt
      721 ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg cgagataggg
      781 gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg
      841 gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgaattcgcc accatgcatc
      901 atcaccatca tcacacgcgt cgcaagaaga ccttcaagga ggtggccacc gccgtgaaga
      961 tcatcgcccg cctgatgggc ctgaagatca acgtctatat caaggccgac aagcagaaga
     1021 acggcatcaa ggcgaacttc cacatccgcc acaacatcga ggacggcggc gtgcagctcg
     1081 cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1141 actacctgag cgtggagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg
     1201 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     1261 gcggtaccgg agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1321 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg
     1381 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1441 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1501 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacatcc
     1561 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     1621 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     1681 gcaacatcct ggggcacaag ctggagtaca acctgccgga ccaactgact gaagagcaga
     1741 tcgcagaatt taaagaggct ttctccctat ttgacaagga cggggatggg acaataacaa
     1801 ccaaggagct ggggacggtg atgcggtctc tcggactgaa ccccacagaa gcagagctgc
     1861 aggacatgat caatgaagta gatgccgacg gtgacggcac aatcgacttc cctgagttcc
     1921 tgacaatgat ggcaagaaaa atgaaataca gggacacgga agaagaaatt agagaagcgt
     1981 tcggtgtgtt tgataaggat ggcaatggct acatcagtgc agcagagctt cgccacgtga
     2041 tgacaaacct tggagagaag ttaacagatg aagaggttga tgaaatgatc agggaagcag
     2101 acatcgatgg ggatggtcag gtaaactacg aagagtttgt acaaatgatg acagcgaagg
     2161 agctcggggg cagcggtggg tctggaggga ctgggggtag cggtggtagc ggtgggacag
     2221 ggggaagcgg cggtagcggt gggacactgg aaatcgaggc cgctttcctg gagcaggaga
     2281 acaccgccct ggagacagag gtggctgagc tggagcagga ggtgcagcgg ctggagaaca
     2341 tcgtgtctca gtacgagacc agatacggac cactgggatc cctggagatc cgggccgctt
     2401 ttctgaggag acggaacacc gccctgagga caagggtggc tgagctgagg cagagagtgc
     2461 agcggctgcg caacatcgtg agccagtacg agacacgcta cggacctctg taaaagctta
     2521 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     2941 tgctcgcctg tgttgccacc tggattctgc gcgggacgcc cttctgctac gtcccttcgg
     3001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     3121 accgtcgact agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3181 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3241 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3301 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3361 gagatctgag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3421 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3481 gagcgagcga gcgcgcagag agggrgkggc cccccccccc cccccccccc tgcagcctgg
     3541 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3601 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3661 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3721 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3781 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3841 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3901 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3961 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4021 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     4081 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     4141 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4201 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4261 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     4321 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     4381 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     4441 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     4501 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     4561 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     4621 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     4681 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     4741 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     4801 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     4861 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     4921 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     4981 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5041 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5101 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5161 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5221 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     5281 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     5341 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     5401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5641 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6121 cg
//
