LOCUS       sequence-358165-        9718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The 3rd generation of rabies vector expressing a 
            tetracycline-controlled transactivator (tTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou 
            Y, Feng G, Wickersham IR
  TITLE     Third-generation rabies viral vectors allow nontoxic retrograde 
            targeting of projection neurons with greatly increased efficiency.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 
            10.1016/j.crmeth.2023.100644.
  PUBMED    37989085
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100644. doi"; pages: "
            10.1016/j.crmeth.2023.100644"
COMMENT     Sequence Label: pRV∆L-5tTA
FEATURES             Location/Qualifiers
     source          1..9718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(171..190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     381..402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     666..688
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     681..697
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        739..1118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1119..1322
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1273..1293
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1367..1386
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1367..1385
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6426..7046
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(7473..7491)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7487..7534
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7557..7573)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7557..7573)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7610..7630)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7610..7628)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7649..7665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7649..7665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7662..7684)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7673..7689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7697..7703,7704..7721,7722..7727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7742..7763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7880..7897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8131..8150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8810..9601,9602..9670))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9433..9452
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9671..9718,1..57))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
       61 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      121 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      181 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      241 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      301 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
      361 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
      421 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
      481 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      541 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      601 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      661 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgataa gcttgatatc
      721 gaattcctgc agcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      781 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      841 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      901 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      961 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1021 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1081 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     1141 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     1201 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     1261 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     1321 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
     1381 atagggagac ccaagctggc tagattaagc gtctgatgag tccgtgagga cgaaacccgg
     1441 cgtaccgggt cacgcttaac aaccagatca aagaaaaaac agacattgtc aattgcaaag
     1501 caaaaatgta acacccctac aatggatgcc gacaagattg tattcaaagt caataatcag
     1561 gtggtctctt tgaagcctga gattatcgtg gatcaatatg agtacaagta ccctgccatc
     1621 aaagatttga aaaagccctg tataacccta ggaaaggctc ccgatttaaa taaagcatac
     1681 aagtcagttt tgtcaggcat gagcgccgcc aaacttaatc ctgacgatgt atgttcctat
     1741 ttggcagcgg caatgcagtt ttttgagggg acatgtccgg aagactggac cagctatgga
     1801 attgtgattg cacgaaaagg agataagatc accccaggtt ctctggtgga gataaaacgt
     1861 actgatgtag aagggaattg ggctctgaca ggaggcatgg aactgacaag agaccccact
     1921 gtccctgagc atgcgtcctt agtcggtctt ctcttgagtc tgtataggtt gagcaaaata
     1981 tccgggcaaa acactggtaa ctataagaca aacattgcag acaggataga gcagattttt
     2041 gagacagccc cttttgttaa aatcgtggaa caccatactc taatgacaac tcacaaaatg
     2101 tgtgctaatt ggagtactat accaaacttc agatttttgg ccggaaccta tgacatgttt
     2161 ttctcccgga ttgagcatct atattcagca atcagagtgg gcacagttgt cactgcttat
     2221 gaagactgtt caggactggt atcatttact gggttcataa aacaaatcaa tctcaccgct
     2281 agagaggcaa tactatattt cttccacaag aactttgagg aagagataag aagaatgttt
     2341 gagccagggc aggagacagc tgttcctcac tcttatttca tccacttccg ttcactaggc
     2401 ttgagtggga aatctcctta ttcatcaaat gctgttggtc acgtgttcaa tctcattcac
     2461 tttgtaggat gctatatggg tcaagtcaga tccctaaatg caacggttat tgctgcatgt
     2521 gctcctcatg aaatgtctgt tctagggggc tatctgggag aggaattctt cgggaaaggg
     2581 acatttgaaa gaagattctt cagagatgag aaagaacttc aagaatacga ggcggctgaa
     2641 ctgacaaaga ctgacgtagc actggcagat gatggaactg tcaactctga cgacgaggac
     2701 tacttttcag gtgaaaccag aagtccggag gctgtttata ctcgaatcat gatgaatgga
     2761 ggtcgactaa agagatctca catacggaga tatgtctcag tcagttccaa tcatcaagcc
     2821 cgtccaaact cattcgccga gtttctaaac aagacatatt cgagtgactc ataagaagtt
     2881 gaataacaaa atgccggaaa tctacggatt gtgtatatcc atcatgaaaa aaactaacac
     2941 ccctcctttc gaaccatccc aaacatgagc aagatctttg tcaatcctag tgctattaga
     3001 gccggtctgg ccgatcttga gatggctgaa gaaactgttg atctgatcaa tagaaatatc
     3061 gaagacaatc aggctcatct ccaaggggaa cccatagagg tggacaatct ccctgaggat
     3121 atggggcgac ttcacctgga tgatggaaaa tcgcccaacc atggtgagat agccaaggtg
     3181 ggagaaggca agtatcgaga ggactttcag atggatgaag gagaggatcc tagcttcctg
     3241 ttccagtcat acctggaaaa tgttggagtc caaatagtca gacaaatgag gtcaggagag
     3301 agatttctca agatatggtc acagaccgta gaagagatta tatcctatgt cgcggtcaac
     3361 tttcccaacc ctccaggaaa gtcttcagag gataaatcaa cccagactac tggccgagag
     3421 ctcaagaagg agacaacacc cactccttct cagagagaaa gccaatcatc gaaagccagg
     3481 atggcggctc aaattgcttc tggccctcca gcccttgaat ggtcggctac caatgaagag
     3541 gatgatctat cagtggaggc tgagatcgct caccagattg cagaaagttt ctccaaaaaa
     3601 tataagtttc cctctcgatc ctcagggata ctcttgtata attttgagca attgaaaatg
     3661 aaccttgatg atatagttaa agaggcaaaa aatgtaccag gtgtgacccg tttagcccat
     3721 gacgggtcca aactccccct aagatgtgta ctgggatggg tcgctttggc caactctaag
     3781 aaattccagt tgttagtcga atccgacaag ctgagtaaaa tcatgcaaga tgacttgaat
     3841 cgctatacat cttgctaacc gaacctctcc cctcagtccc tctagacaat aaaatccgag
     3901 atgtcccaaa gtcaacatga aaaaaacagg caacaccact gataaaatga acctcctacg
     3961 taagatagtg aaaaaccgca gggacgagga cactcaaaaa tcctctcccg cgtcagcccc
     4021 tctggatgac gatgacttgt ggcttccacc ccctgaatac gtcccgctga aagaacttac
     4081 aggcaagaag aacatgagga acttttgtat caacggaagg gttaaagtgt gtagcccgaa
     4141 tggttactcg ttcaggatcc tgcggcacat tctgaaatca ttcgacgaga tatattctgg
     4201 gaatcatagg atgatcgggt tagtcaaagt ggttattgga ctggctttgt caggatctcc
     4261 agtccctgag ggcctgaact gggtatacaa attgaggaga acctttatct tccagtgggc
     4321 tgattccagg ggccctcttg aaggggagga gttggaatac tctcaggaga tcacttggga
     4381 tgatgatact gagttcgtcg gattgcaaat aagagtgatt gcaaaacagt gtcatatcca
     4441 gggcagagtc tggtgtatca acatgaaccc gagagcatgt caactatggt ctgacatgtc
     4501 tcttcagaca caaaggtccg aagaggacaa agattcctct ctgcttctag aataatcaga
     4561 ttatatcccg caaatttatc acttgtttac ctctggagga gagaacatat gggctcaact
     4621 ccaacccttg ggagcaatat aacaaaaaac atgttatggt gccattaaac cgctgcattt
     4681 catcaaagtc aagttgatta cctttacatt ttgatcctct tggatgtgaa aaaaactatt
     4741 aacatccctc aaaagaggcg cgccaaggaa agatggttcc tcaggctctc ctgtttgtac
     4801 cccttctggt ttttccattg tgttttggga aattccctat ttacacgata ccagacaagc
     4861 ttggtccctg gagtccgatt gacatacatc acctcagctg cccaaacaat ttggtagtgg
     4921 aggacgaagg atgcaccaac ctgtcagggt tctcctacat ggaacttaaa gttggataca
     4981 tcttagccat aaaagtgaac gggttcactt gcacaggcgt tgtgacggag gctgaaacct
     5041 acactaactt cgttggttat gtcacaacca cgttcaaaag aaagcatttc cgcccaacac
     5101 cagatgcatg tagagccgcg tacaactgga agatggccgg tgaccccaga tatgaagagt
     5161 ctctacacaa tccgtaccct gactaccgct ggcttcgaac tgtaaaaacc accaaggagt
     5221 ctctcgttat catatctcca agtgtggcag atttggaccc atatgacaga tcccttcact
     5281 cgagggtctt ccctagcggg aagtgctcag gagtagcggt gtcttctacc tactgctcca
     5341 ctaaccacga ttacaccatt tggatgcccg agaatccgag actagggatg tcttgtgaca
     5401 tttttaccaa tagtagaggg aagagagcat ccaaagggag tgagacttgc ggctttgtag
     5461 atgaaagagg cctatataag tctttaaaag gagcatgcaa actcaagtta tgtggagttc
     5521 taggacttag acttatggat ggaacatggg tctcgatgca aacatcaaat gaaaccaaat
     5581 ggtgccctcc cgataagttg gtgaacctgc acgactttcg ctcagacgaa attgagcacc
     5641 ttgttgtaga ggagttggtc aggaagagag aggagtgtct ggatgcacta gagtccatca
     5701 tgacaaccaa gtcagtgagt ttcagacgtc tcagtcattt aagaaaactt gtccctgggt
     5761 ttggaaaagc atataccata ttcaacaaga ccttgatgga agccgatgct cactacaagt
     5821 cagtcagaac ttggaatgag atcctccctt caaaagggtg tttaagagtt ggggggaggt
     5881 gtcatcctca tgtgaacggg gtgtttttca atggtataat attaggacct gacggcaatg
     5941 tcttaatccc agagatgcaa tcatccctcc tccagcaaca tatggagttg ttggaatcct
     6001 cggttatccc ccttgtgcac cccctggcag acccgtctac cgttttcaag gacggtgacg
     6061 aggctgagga ttttgttgaa gttcaccttc ccgatgtgca caatcaggtc tcaggagttg
     6121 acttgggtct cccgaactgg gggaagtatg tattactgag tgcaggggcc ctgactgcct
     6181 tgatgttgat aattttcctg atgacatgtt gtagaagagt caatcgatca gaacctacgc
     6241 aacacaatct cagagggaca gggagggagg tgtcagtcac tccccaaagc gggaagatca
     6301 tatcttcatg ggaatcacac aagagtgggg gtgagaccag actgtaaggt accggactgg
     6361 ccgtcctttc aacgatccaa gtccatgaaa aaaactaaca cccctccgct agcaaagccg
     6421 ccaccatgtc cagactggac aagagcaaag tcatcaactc tgccttggag ctcctgaatg
     6481 aagttggcat tgagggcttg accaccagga agctggccca gaagctgggt gtggagcagc
     6541 ctaccctgta ctggcatgtg aagaacaaga gggctctgct tgatgccctg gccattgaga
     6601 tgttggacag gcaccacacc cacttctgcc ctctggaagg ggagtcctgg caggacttcc
     6661 tgaggaacaa tgccaagagc ttcagatgtg ccttgctctc ccaccgggat ggtgccaaag
     6721 ttcacttggg caccaggcct acagagaagc agtatgagac cctggagaac cagctggcat
     6781 tcctgtgcca acaaggcttc tccctggaaa atgccttgta tgccctctct gctgtgggcc
     6841 acttcacctt gggctgtgtg ctggaggacc aggagcacca agttgccaag gaggagaggg
     6901 agacccccac cactgactcc atgccaccac tgctgcggca agctattgag ttgtttgacc
     6961 accaaggggc tgagcctgca ttcctttttg gcctggaact gatcatctgt ggcctggaaa
     7021 agcagctgaa atgtgagtct ggctctcacg tgcccaaaaa gagaaagcac gtgcctgctg
     7081 atgccttgga tgattttgac ctggacatgc tgcctgctga tgccctggat gactttgatt
     7141 tggacatgct ccctgctgat gcacttgatg attttgacct ggatatgctg tgatgacgta
     7201 cgaccggata ctcctggaag cctgcccatg ctaagactct tgtgtgatgt atcttgaaaa
     7261 aaacaagatc ctaaatctga acctttggtt gtttgattgt ttttctcatt tttgttgttt
     7321 atttgttaag cgtgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc
     7381 gaaggaggac gcacgtccac tcggatggct aagggagggc ggggatccgg ctgctaacaa
     7441 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     7501 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc
     7561 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     7621 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7681 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7741 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7801 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7861 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7921 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7981 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8041 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8101 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8161 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8221 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8281 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8341 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8401 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8461 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8521 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8581 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8641 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8701 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8761 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8821 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8881 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8941 tgataccgcg agacccaccc ttggaggctc cagatttatc agcaataaac cagccagccg
     9001 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9061 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9121 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9181 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9241 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9301 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9361 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9421 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9481 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9541 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9601 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9661 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtct
//
