LOCUS       sequence-358181-        4866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence of human FAM3B in pCS2 with C terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Zhang F, Zhu X, Wang P, He Q, Huang H, Zheng T, Li Y, Jia H, Xu L, 
            Zhao H, Colozza G, Tao Q, De Robertis EM, Ding Y
  TITLE     The cytokine FAM3B/PANDER is an FGFR ligand that promotes posterior 
            development in Xenopus.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2100342118. doi:
            10.1073/pnas.2100342118.
  PUBMED    33975953
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-18"; volume: "118(20). pii: 
            2100342118. doi"; pages: " 10.1073/pnas.2100342118"
COMMENT     Sequence Label: pCS2-hFAM3B-FLAG
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..1003
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1038..1056
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1038..1055
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1809..1880
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     polyA_signal    1921..2055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1945..1964)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1999..2018
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2168..2188)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2168..2186)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2207..2223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2207..2223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2220..2242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2231..2247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2255..2261,2262..2279,2280..2285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2300..2321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2438..2455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2609..3197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2689..2708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3368..4159,4160..4228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3991..4010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4229..4333)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4359..4814)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4496..4517)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4708..4727
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg cgtatatgga ctcatgccaa
       61 ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg cgtatatgga ctcgtgccaa
      121 ttcaatatgg tggatctgga ccccagccaa ttcaatatgg cggacttggc accatgccaa
      181 ttcaatatgg cggacctggc actgtgccaa ctggggaggg gtctacttgg cacggtgcca
      241 agtttgagga ggggtcttgg ccctgtgcca agtccgccat attgaattgg catggtgcca
      301 ataatggcgg ccatattggc tatatgccag gatcaatata taggcaatat ccaatatggc
      361 cctatgccaa tatggctatt ggccaggttc aatactatgt attggcccta tgccatatag
      421 tattccatat atgggttttc ctattgacgt agatagcccc tcccaatggg cggtcccata
      481 taccatatat ggggcttcct aataccgccc atagccactc ccccattgac gtcaatggtc
      541 tctatatatg gtctttccta ttgacgtcat atgggcggtc ctattgacgt atatggcgcc
      601 tcccccattg acgtcaatta cggtaaatgg cccgcctggc tcaatgccca ttgacgtcaa
      661 taggaccacc caccattgac gtcaatggga tggctcattg cccattcata tccgttctca
      721 cgccccctat tgacgtcaat gacggtaaat ggcccacttg gcagtacatc aatatctatt
      781 aatagtaact tggcaagtac attactattg gaagtacgcc agggtacatt ggcagtactc
      841 ccattgacgt caatggcggt aaatggcccg cgatggctgc caagtacatc cccattgacg
      901 tcaatgggga ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg
      961 tgcctaatgg gaggtctata taagcaatgc tcgtttaggg aaccgccatt ctgcctgggg
     1021 acgtcggagc aagcttgatt taggtgacac tatagaatac aagctacttg ttctttttgc
     1081 aggatcccat cgataccatg cgcccattgg ctggtggcct gctcaaggtg gtgttcgtgg
     1141 tcttcgcctc cttgtgtgcc tggtattcgg ggtacctgct cgcagagctc attccagatg
     1201 cacccctgtc cagtgctgcc tatagcatcc gcagcatcgg ggagaggcct gtcctcaaag
     1261 ctccagtccc caaaaggcaa aaatgtgacc actggactcc ctgcccatct gacacctatg
     1321 cctacaggtt actcagcgga ggtggcagaa gcaagtacgc caaaatctgc tttgaggata
     1381 acctacttat gggagaacag ctgggaaatg ttgccagagg aataaacatt gccattgtca
     1441 actatgtaac tgggaatgtg acagcaacac gatgttttga tatgtatgaa ggcgataact
     1501 ctggaccgat gacaaagttt attcagagtg ctgctccaaa atccctgctc ttcatggtga
     1561 cctatgacga cggaagcaca agactgaata acgatgccaa gaatgccata gaagcacttg
     1621 gaagtaaaga aatcaggaac atgaaattca ggtctagctg ggtatttatt gcagcaaaag
     1681 gcttggaact cccttccgaa attcagagag aaaagatcaa ccactctgat gctaagaaca
     1741 acagatattc tggctggcct gcagagatcc agatagaagg ctgcataccc aaagaacgaa
     1801 gcgatcctga ttataaagat gacgatgaca aggattataa agatgacgat gacaaggatt
     1861 ataaagatga cgatgacaag taactcgagc ctctagaact atagtgagtc gtattacgta
     1921 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1981 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2041 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2101 gtgtgggagg ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct
     2161 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     2221 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt
     2281 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2341 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2401 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2581 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2641 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2701 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2761 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2821 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2881 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2941 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3001 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3061 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4321 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     4381 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     4441 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     4501 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     4561 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     4621 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     4681 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     4741 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     4801 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4861 gcgggc
//
