LOCUS       sequence-358183-        9845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin 
            Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 
            homology arms for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT IAA17-6FLAG
FEATURES             Location/Qualifiers
     source          1..9845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..3335
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             3381..3404
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3411..3434
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3441..3464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3471..3494
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3501..3524
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3531..3554
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        4029..4430
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4452..4472
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5010..5036
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5046..5102
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5139..5159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5175..6896
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(7072..7092)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7072..7090)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7111..7127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8272..9063,9064..9132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8895..8914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9133..9237)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9274..9777
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9818..9836
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atccggctct gttgagttaa acctgcgtga gaccgagcta tgtcttggtc
     2701 tacccggtgg tgacactgta gcaccagtga caggcaacaa aaggggcttc agcgagactg
     2761 tagatctgaa gctaaactta aacaacgaac cagctaataa ggaaggtagt acaacacatg
     2821 atgtggttac gtttgacagc aaggagaaga gtgcctgtcc caaagatccg gcaaagcccc
     2881 cggcgaaggc ccaggttgta ggttggcctc cggtaaggtc ctatcgtaaa aacgtaatgg
     2941 ttagttgtca gaagtccagc ggaggacctg aagcagcggc gttcgtcaaa gtatctatgg
     3001 atggcgcccc atacttgaga aagatagacc tgcgtatgta taagtcttac gacgagttat
     3061 caaatgcgct atctaacatg ttcagcagtt tcaccatggg caaacatggt ggtgaggagg
     3121 gaatgatcga cttcatgaac gagagaaaac ttatggacct ggtgaatagc tgggattatg
     3181 tccctagcta cgaggataaa gacggggatt ggatgcttgt gggggatgtt ccgtggccga
     3241 tgttcgtcga tacgtgtaag cgtctgcgtc taatgaaggg ctccgatgct attggtttag
     3301 ctccgcgtgc tatggaaaag tgtaagtcca gagctgtatc acccgggata cattatacga
     3361 agttatgcat gctcactagt gactacaagg acgacgatga caagtctagt gactacaagg
     3421 acgacgatga caagtctagt gactacaagg acgacgatga caagtctagt gactacaagg
     3481 acgacgatga caagtctagt gactacaagg acgacgatga caagtctagt gactacaagg
     3541 acgacgatga caagtctaga tgaggcgcgc tagatctgtt tagcttaaga gttcttcttt
     3601 gagatatcga ttgaacgagc tcgaattcat cgatctatat taccctgtta tccctagcgt
     3661 aactcatatg tacaatgagc cggtcacaac cctctttcac gacgttgaag cttcacaaac
     3721 acaccacaga cgtgggtcaa tcgggtgtac aatatggact tcctcttttc tggcaaccaa
     3781 acccatacat cgggattcct ataatacctt cgttggtctc cctaacatgt aggtggcgga
     3841 ggggagatat acaatagaac agataccaga caagacataa tgggctaaac aagactacac
     3901 caattacact gcctcattga tggtggtaca taacgaacta atactgtagc cctagacttg
     3961 atagccatca tcatatcgaa gtttcactac cctttttcca tttgccatct attgaagtaa
     4021 taataggcgc atgcaacttc ttttcttttt ttttcttttc tctctccccc gttgttgtct
     4081 caccatatcc gcaatgacaa aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa
     4141 agacagcacc aacagatgtc gttgttccag agctgatgag gggtatctcg aagcacacga
     4201 aactttttcc ttccttcatt cacgcacact actctctaat gagcaacggt atacggcctt
     4261 ccttccagtt acttgaattt gaaataaaaa aaagtttgct gtcttgctat caagtataaa
     4321 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
     4381 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact atctcatata
     4441 caactagaat gcccaagaag aaacgtaagg tgggttccac ccttacgagc ggtcaacgtg
     4501 accacagaga cgttgaaaat gagttatcca gtgcagcttt tagcagtcaa agtttcgggg
     4561 ttccagatat gtcattcaaa ccggggtgct ccagtgacgt cgcagtcact gatgcgggta
     4621 tgcctagcca agggttatgg aacaaccaga cgcaaaggat gcgtaccttc actaaggtcc
     4681 agaaaagggg ctctgttggg aggagtatcg acatcaccag gtatcgtgat tacgatgagc
     4741 tacgtcatga tctggcatgt atgttcggca tacaggggca gttagaagac ccttatcgta
     4801 tggattggaa gttagtttat gtggatcacg agaacgatat actgttggtg ggcgacgacc
     4861 cgtgggagga atttgtaggt tgtgttaagt ctatcaagat tctatctgct gcagaggtgc
     4921 agcaaatgag cttggacggg gatcttggtg gagttccgcc ccaaacccag gcgtgcagtg
     4981 cttcagacga tgcgaacgct tggagaggct atccctacga cgtgcctgat tatgcgggta
     5041 gcggggccac taacttcagc ttgttgaagc aggctggcga tgtggaggag aatccaggtc
     5101 cggcggctac cgataaagcg gaattaattc ccgagcctcc aaaaaagaag agaaaggtcg
     5161 aattgggtac cgccacgtac ttcccggagg aggtggtgga gcacatcttc agcttcctgc
     5221 cggcgcagcg cgaccgcaac acggtctcgc tcgtctgcaa ggtgtggtac gagatcgaga
     5281 ggctgagccg ccgcggcgtc ttcgtgggca actgctacgc cgtgcgcgcc ggccgcgtcg
     5341 ccgcgcggtt ccccaacgtg cgggcgctca cggtgaaggg gaagccccac ttcgccgact
     5401 tcaacctcgt gccccccgac tggggcggct acgcggggcc gtggatcgag gcggccgcga
     5461 ggggatgcca cggcctggag gagctcagga tgaagcggat ggtggtgtcc gacgagagcc
     5521 tcgagctgct ggctcgctcg ttcccgcggt tcagggctct tgttcttatc agctgcgagg
     5581 ggttcagcac tgacgggcta gccgccgtcg cgagccattg caagcttctg agggagttgg
     5641 atttgcagga aaatgaagtg gaggatcgag ggcctaggtg gctttcctgc ttccctgatt
     5701 cctgcacatc acttgtctca ttgaattttg cctgcatcaa aggggaggtt aatgctggtt
     5761 cactggagag acttgttagc aggtccccaa acctgcggag tttgaggctg aatcgatctg
     5821 tatcggtaga tacacttgca aagatactac tgcgtacccc taacttggag gatttgggga
     5881 cagggaattt gacagatgac ttccaaactg agtcctactt taagcttacc agtgctctgg
     5941 agaaatgcaa gatgttgagg agtttgtctg gattctggga tgcttctcct gtttgcctgt
     6001 catttatcta ccccctgtgt gctcaactga caggattgaa cttgagctat gcacccacac
     6061 ttgatgcttc tgaccttaca aaaatgatta gccgctgtgt gaagctccaa cgcctttggg
     6121 tactggattg tatctcggac aaaggcttgc aagtggtggc ctccagttgc aaagacttgc
     6181 aagaactcag ggtatttcca tcagatttct acgtagctgg ttattctgca gtgacagagg
     6241 agggacttgt tgcagtatcc ttgggctgtc caaaactgaa ctcactactg tacttctgtc
     6301 accaaatgac taatgctgca ctagttactg tcgccaagaa ctgtccaaat ttcacacgat
     6361 tcagactttg tattcttgag ccagggaagc ctgatgttgt gacaagccaa ccattagatg
     6421 aaggctttgg agctattgtt cgtgagtgca agggattaca acgtttgtca atatctggtc
     6481 ttctcacaga caaagttttc atgtatattg ggaaatatgc aaaacaactt gagatgcttt
     6541 ctatagcatt tgctggtgac agtgataagg gtatgatgca tgttatgaat ggatgcaaga
     6601 atttaaggaa actggagata agagatagcc cgtttggtga tgctgcactc ttggggaatt
     6661 ttgctaggta cgagacaatg cgatcccttt ggatgtcatc ttgcaatgtc acgttaaagg
     6721 ggtgccaagt ccttgcgtca aagatgccga tgctcaatgt tgaggtcata aatgagcggg
     6781 atggtagcaa tgaaatggag gaaaaccatg gagatctgcc taaagtggag aaattatatg
     6841 tgtaccgcac aactgctggg gcgagggatg atgcaccaaa ttttgttaaa atcctagtcg
     6901 actccggttc tgctgctagt ggttgaacgc gtggaatgtt gtagcataga tatgacgttt
     6961 tattaccttt gatcacattt ccacgccatt tcgcattctc accctcctat attaccctgt
     7021 tatccctagc gtaactcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     7081 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9241 atttccccga aaagtgccac ctgggtcctt ttcatcacgt gctataaaaa taattataat
     9301 ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag aaattaaaga
     9361 aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc aacaaatact
     9421 accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc atcttgtctg
     9481 tgtagaagac cacacacgaa aatcctgtga ttttacattt tacttatcgt taatcgaatg
     9541 tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta cgctttttgt
     9601 tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct tctaccttct
     9661 ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca gagtaaattc
     9721 ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca agcgatccgt
     9781 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9841 tcgtc
//
