LOCUS       sequence-358238-        4488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing FOXN1 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-FOXN1
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2045..2144)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2161..2180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2162..2180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2303..2322)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2314..3123
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2381..2400)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3270..3858
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3759..3778
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4188..4215)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4307..4393)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4442..4464
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4456..4473
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4457..4473
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggtgagcct gcctcccccc
      121 cagtctgacg tcactctgcc cggacccaca agactggaag gagagagaca gggcgatttg
      181 atgcaggctc ctggattacc cggaagccct gcacctcaga gcaagcacgc tggcttctct
      241 tgctccagct tcgtgagcga cggaccccca gagagaacac catctcttcc ccctcactct
      301 cccagaattg cctctcccgg acctgaacaa gtgcagggac actgtcctgc tggcccagga
      361 cccggcccat tcagacttag cccttctgac aagtaccccg gattcggatt cgaagaggca
      421 gctgctagct cccctggcag attccttaag ggcagccacg ctcccttcca cccctataag
      481 aggccctttc acgaggatgt gttccccgag gccgagacaa ccctggcctt aaagggccac
      541 agctttaaga cccccggccc tctggaggcc tttgaagaga tccctgtgga tgttgccgaa
      601 gccgaggctt tccttcctgg ctttagcgct gaggcctggt gcaatggact gccttatccc
      661 tctcaggagc acggccctca ggtgcttgga agcgaagtga aagtgaagcc ccccgtgctg
      721 gaaagcggcg ctggcatgtt ttgctaccag cctcctctgc agcacatgta ctgcagcagc
      781 cagccacctt tccaccagta cagccctggc ggcggatctt atcctatccc ctacctgggc
      841 tctagccact accagtacca gagaatggcc cctcaagcca gcaccgatgg gcatcagccc
      901 ctgttcccca agcccatcta cagctacagc atcctgatct tcatggccct gaagaatagc
      961 aagaccggca gcctgccagt gagcgagatc tacaacttca tgaccgagca cttcccctac
     1021 ttcaagaccg cccccgacgg atggaagaac agcgtgagac acaacctgag cctgaataag
     1081 tgcttcgaga aggtggagaa caagagcggc tccagcagca gaaagggctg cctgtgggcc
     1141 ctcaaccccg ccaagatcga caagatgcag gaggaactgc agaagtggaa gagaaaggac
     1201 cccatcgccg tgagaaagag catggccaag cctgaggagc tcgacagcct gatcggcgac
     1261 aagagagaaa aactgggctc acctctgctg ggatgccccc ctcccggcct ttctggatct
     1321 ggacctatta gaccccttgc ccctccagct ggtttgagcc ctcctttaca ctctctgcat
     1381 cctgctcctg gccccatccc tggcaagaac ccccttcagg atctcctgat gggccataca
     1441 cccagctgtt acggccagac atacctccat ctgtccccag gccttgctcc tcctggaccc
     1501 cctcagcctc tgtttcccca gccagatgga catcttgaac tgagagctca gcctggaacc
     1561 ccccaggatt ctccactgcc cgctcataca cctcccagcc acagcgccaa actgctggct
     1621 gagccttctc ctgccagaac aatgcacgat acactgctgc ctgatggcga cctgggcacc
     1681 gatctggatg ccatcaaccc cagcctgaca gacttcgact tccagggcaa cctgtgggag
     1741 cagctgaagg acgattcact ggccctggat ccccttgtgc tggtgaccag cagccctaca
     1801 tctagcagca tgcctcctcc tcagccccct cctcattgtt tccctccagg accttgtctg
     1861 acagagactg gctctggggc tggcgattta gcagctccag gatctggcgg ctctggagct
     1921 ctgggcgatc tgcatctcac aaccctctac tcagccttta tggagctgga acctacaccc
     1981 cctacagctc ccgccggacc tagcgtgtac ctgtctcctt ctagcaagcc cgtggccctc
     2041 gcctgcccaa ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     2101 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     2161 ccctatagtg agtcgtatta catggtcata gctgtttctt ggcagctctg gcccgtgtct
     2221 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     2281 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     2341 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     2401 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     2461 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     2521 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     2581 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     2641 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     2701 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     2761 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     2821 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     2881 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2941 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     3001 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     3061 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     3121 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     3181 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     3241 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3301 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3361 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3421 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3481 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3541 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3601 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3661 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3721 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3781 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3841 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3901 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3961 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4021 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4081 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4141 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     4201 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     4261 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     4321 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     4381 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     4441 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
