LOCUS       sequence-358243-        4665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF30 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF30
FEATURES             Location/Qualifiers
     source          1..4665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1976..2075)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2092..2111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2093..2111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2116..2132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2116..2132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2563..3354
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3447..4035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3936..3955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4189..4206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4365..4392)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4484..4570)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4619..4641
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4633..4650
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4634..4650
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatggccca taaatatgtg
      121 ggtttgcagt atcacggatc agtgacattt gaggatgtgg ccatagcctt ctcccagcag
      181 gagtgggaga gtctggactc ttcccagagg ggcttgtaca gagatgtgat gttggagaac
      241 tacaggaact tggtgtcaat ggcaggacat tcccgttcta aaccacatgt gatcgcctta
      301 ttggaacaat ggaaagagcc tgaagtgaca gtgaggaaag atggaagaag atggtgcaca
      361 gatttgcagt tggaagatga tacaatcggc tgtaaagaaa tgcccacctc tgaaaactgt
      421 ccatcttttg ctctacatca gaaaataagt agacagaaac cacgtgaatg tcaggaatat
      481 ggaaagaccc tttgtcaaga ctcaaagcct gttcaacatg aaagaataca tagtagtgaa
      541 aaacccaaca gatgtaaaga atgtgggaag aactttagta atggacatca actcaccata
      601 catcagagat tgcatgttgg tgagaaaccc tataaatatg aaaaatgtgg gaaggccttt
      661 atcagtggct cagcctttgt taagcatggg agaattcaca ctggtgagaa gccactcaaa
      721 tgtaagcaat gtggaaagac tattagtggt agctatcaac ttacagtaca taagagtatt
      781 catactggga agaaaccata tgagtgcggg gaatgtggga aagcttttct agtatatgga
      841 aagcttaccc ggcatcagag tactcacact ggtgaaaaac cctttgggtg tgaggagtgt
      901 gggaaggcct tcagtacctt ttcatacctg gttcaacatc agcgaattca taccagtgaa
      961 aaaccttacg aatgcaaaga atgtgggaag gcctttagca ctagctcacc ccttgctaag
     1021 catcagagaa ttcatactgg cgagaaaccc tatgaatgta aggagtgtgg gaagtccttc
     1081 actgtgtatg gacagcttac tcgacatcag agtattcata ctggtgagaa accttttgaa
     1141 tgtaaggaat gtggaaaggc ctttagactt agttccttcc ttcatgcaca tcagcgaatt
     1201 catgcagaga taaagcccta cggatgcaag gaatgcggga gaaccttcag tcgtgcctca
     1261 tatcttgttc aacatggaag acttcacact ggcgagaagc cctatgaatg taaggagtgt
     1321 ggcaaggcct ttagtactgg ctcatacctt gttcagcatc agaggatcca tactggggag
     1381 aaaccctatg aatgtaagga atgtggcaaa gcctttatta gtcgccatca gcttaccgta
     1441 catcaaaggg ttcatactgg agagaaaccc tatgagtgta aggaatgtgg caaggccttc
     1501 agagtgcacg tacatctcac acagcatcgg aaaattcata ctgatgtaaa gccctatgaa
     1561 tgtaaggaat gtggaaaggc ttttagtcga gcctcgtacc ttgtacaaca tagcagaatc
     1621 catactggta agaagcccta tgagtgtaag gagtgtggca aggccttcag ttctggctca
     1681 taccttgttc agcatcaaag aattcatact ggggagaaac cctatgaatg taacaaatgt
     1741 gggaaagcct ttactgttta tggacaactt attggacatc agagtgttca cactggtgag
     1801 aaaccttttg aatgtaagga atgcgggaag gcctttagac ttaattcatt ccttactgaa
     1861 catcagcggg tacacactgg tgagaaaccc tttaaatgca aaaaatgtgg gaagaccttt
     1921 agatacagtt cagcccttaa agtgcatctg agaaaacata tgagtgttat acccttgcca
     1981 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2041 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2101 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2161 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     2221 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     2281 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     2341 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2401 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     2461 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2521 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     2581 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2641 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     2701 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2761 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2821 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2881 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2941 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3001 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3061 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3121 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3181 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     3241 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     3301 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     3361 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     3421 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3481 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3541 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3601 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3661 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3721 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3781 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3841 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3901 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3961 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4021 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4081 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4141 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4201 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     4261 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     4321 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     4381 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     4441 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     4501 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     4561 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     4621 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
