LOCUS       sequence-358247-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of human DHFR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Bhabha G, Tuttle L, Martinez-Yamout MA, Wright PE
  TITLE     Identification of endogenous ligands bound to bacterially expressed 
            human and E. coli dihydrofolate reductase by 2D NMR.
  JOURNAL   FEBS Lett. 2011 Nov 16;585(22):3528-32. Epub 2011 Oct 20.
  PUBMED    22024482
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS 
            Lett."; date: "2011-11-16"; volume: "585(22)"; pages: "3528-32. Epub
            2011 Oct 20"
COMMENT     Sequence Label: hDHFR
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..109
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        90..108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    109..133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             148..170
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             790..807
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(860..878)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
       61 agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc
      121 ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
      181 gttggttcgc taaactgcat cgtcgctgtg tcccagaaca tgggcatcgg caagaacggg
      241 gacctgccct ggccaccgct caggaatgaa ttcagatatt tccagagaat gaccacaacc
      301 tcttcagtag aaggtaaaca gaatctggtg attatgggta agaagacctg gttctccatt
      361 cctgagaaga atcgaccttt aaagggtaga attaatttag ttctcagcag agaactcaag
      421 gaacctccac aaggagctca ttttctttcc agaagtctag atgatgcctt aaaacttact
      481 gaacaaccag aattagcaaa taaagtagac atggtctgga tagttggtgg cagttctgtt
      541 tataaggaag ccatgaatca cccaggccat cttaaactat ttgtgacaag gatcatgcaa
      601 gactttgaaa gtgacacgtt ttttccagaa attgatttgg agaaatataa acttctgcca
      661 gaatacccag gtgttctctc tgatgtccag gaggagaaag gcattaagta caaatttgaa
      721 gtatatgaga agaatgatta ataggatccg aattcgagct ccgtcgacaa gcttgcggcc
      781 gcactcgagc accaccacca ccaccactga agatccggct gctacaaagc ccgaaaggaa
      841 gctgagttgg gtgctgccac cgctgagcaa taactagcat
//
