LOCUS       sequence-358251-        7239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the myc-tagged catalytic domains of wild-type SHP-1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Alborzian Deh Sheikh A, Akatsu C, Abdu-Allah HHM, Suganuma Y, 
            Imamura A, Ando H, Takematsu H, Ishida H, Tsubata T
  TITLE     The Protein Tyrosine Phosphatase SHP-1 (PTPN6) but Not CD45 (PTPRC) 
            Is Essential for the Ligand-Mediated Regulation of CD22 in 
            BCR-Ligated B Cells.
  JOURNAL   J Immunol. 2021 Jun 1;206(11):2544-2551. doi: 
            10.4049/jimmunol.2100109. Epub 2021 May 14.
  PUBMED    33990399
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.2100109"; journalName: "J Immunol"; date: 
            "2021-06-1- 1"; volume: "206"; issue: "11"; pages: "2544-2551"
COMMENT     Sequence Label: pMX-SHP1C-ires-hCD8
FEATURES             Location/Qualifiers
     source          1..7239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1923..1932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1938..2027
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             1968..2057
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             1998..2087
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             2028..2117
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             2058..2147
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     misc_feature    join(3376..3950,3951..3953,3954..3962)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3543..3560)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3770..3789
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4745..5214
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5344..5361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5515..6103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5595..5614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6267..7058,7059..7127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6890..6909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7128..7232)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccaccat ggggtcggaa cagaaactta tttctgaaga agatcttgaa cagaaactta
     1981 tttctgaaga agatcttgaa cagaaactta tttctgaaga agatcttgaa cagaaactta
     2041 tttctgaaga agatcttgaa cagaaactta tttctgaaga agatcttgaa cagaaactta
     2101 tttctgaaga agatcttgaa cagaaactta tttctgaaga agatcttcga cccggtggaa
     2161 tcgatgcctt tgtctacctg cggcagcctt actacgctac tcgggtgaac gcagctgaca
     2221 ttgagaatcg ggtcttggaa ctgaacaaga agcaggagtc ggaggacaca gccaaggctg
     2281 gcttctggga ggagtttgag agtctacaaa agcaggaggt aaagaatcta caccaacgtc
     2341 tggaagggca gcggccagag aacaagagca agaaccgcta caagaacatt cttccctttg
     2401 accacagccg agtgatcctg cagggacgtg acagtaacat cccaggctct gactacatca
     2461 atgccaacta cgtgaagaac cagctgctag gtccagatga gaactctaag acctacatcg
     2521 ccagccaggg ctgtctggat gccacagtca atgacttctg gcagatggct tggcaggaga
     2581 acactcgtgt catcgtcatg actaccagag aggtggagaa aggccggaac aaatgtgtcc
     2641 catactggcc cgaggtgggc actcagcgtg tctatggtct ctactctgtg accaacagta
     2701 gggagcatga cacagcagaa tacaaactgc gaacattaca gatctcccca ctagacaatg
     2761 gggacctggt tcgggagata tggcactacc agtacctgag ctggcctgac catggggttc
     2821 ccagtgagcc tgggggtgtc ctcagctttc tggatcagat caaccagcga caggaaagtt
     2881 tgcctcatgc agggcccatc attgtgcatt gcagcgctgg catcggccgc acgggcacca
     2941 tcatcgtcat tgatatgctt atggaaagca tctccaccaa ggggctagac tgtgacattg
     3001 atatccagaa gaccatccag atggtacgag cacagcgctc cggcatggtg cagaccgagg
     3061 cccagtacaa gtttatttac gtggccattg cccagttcat cgaaacgacc aagaagaaac
     3121 tggagatcat acaatcccag aagggccagg agtcggagta tgggaatatc acgtaccctc
     3181 ccgctgtgag gagtgcccac gccaaagcct cgcgtacttc ctccaagcac aaggaggagg
     3241 tgtacgaaaa cgtgcatagc aagagcaaga aggaagagaa agtaaagaag cagcggtcgg
     3301 cagacaagga gaagaacaaa ggttctctca agaggaagtg acgccgcggc cgctacgtaa
     3361 attccgcccc ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt
     3421 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     3481 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     3541 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     3601 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     3661 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     3721 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     3781 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     3841 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     3901 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     3961 ccatggcctt accagtgacc gccttgctcc tgccgctggc cttgctgctc cacgccgcca
     4021 ggccgagcca gttccgggtg tcgccgctgg atcggacctg gaacctgggc gagacagtgg
     4081 agctgaagtg ccaggtgctg ctgtccaacc cgacgtcggg ctgctcgtgg ctcttccagc
     4141 cgcgcggcgc cgccgccagt cccaccttcc tcctatacct ctcccaaaac aagcccaagg
     4201 cggccgaggg gctggacacc cagcggttct cgggcaagag gttgggggac accttcgtcc
     4261 tcaccctgag cgacttccgc cgagagaacg agggctacta tttctgctcg gccctgagca
     4321 actccatcat gtacttcagc cacttcgtgc cggtcttcct gccagcgaag cccaccacga
     4381 cgccagcgcc gcgaccacca acaccggcgc ccaccatcgc gtcgcagccc ctgtccctgc
     4441 gcccagaggc gtgccggcca gcggcggggg gcgcagtgca cacgaggggg ctggacttcg
     4501 cctgtgatat ctacatctgg gcgcccttgg ccgggacttg tggggtcctt ctcctgtcac
     4561 tggttatcac ctaactcgac gataaaataa aagattttat ttagtctcca gaaaaagggg
     4621 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     4681 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
     4741 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     4801 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     4861 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt
     4921 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     4981 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     5041 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc
     5101 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
     5161 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca
     5221 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt
     5281 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6181 attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt
     6241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7201 atttagaaaa ataaacaaat aggggttccg cgcacattt
//
