LOCUS       sequence-358253-        8506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fusion protein containing the extracellular part of 
            human Siglec-10 and the Fc region of human IgG1, and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Alborzian Deh Sheikh A, Gomaa S, Li X, Routledge M, Saigoh K, Numoto
            N, Angata T, Hitomi Y, Takematsu H, Tsuiji M, Ito N, Kusunoki S, 
            Tsubata T
  TITLE     A Guillain-Barre syndrome-associated SIGLEC10 rare variant impairs 
            its recognition of gangliosides.
  JOURNAL   J Autoimmun. 2021 Jan;116:102571. doi: 10.1016/j.jaut.2020.102571. 
            Epub 2020 Nov 19.
  PUBMED    33223341
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Autoimmun."; date: "2021-01"; volume: "116:102571. doi"; pages: " 
            10.1016/j.jaut.2020.102571. Epub 2020 Nov 19"
COMMENT     Sequence Label: pMXs-Sig10-Fc-IG
FEATURES             Location/Qualifiers
     source          1..8506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    4558..5109
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4701..4718)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4928..4947
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5121..5123,5124..5126,5127..5840)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5121..5123,5124..5126,5127..5837)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5166..5187)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5427..5446)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5774..5795
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6011..6480
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6610..6627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6781..7369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6861..6880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7533..8324,8325..8393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8156..8175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8394..8498)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgagctc ggtacctcgc gaatgcatct agagccacca
     1981 tgctactgcc actgctgctg tcctcgctgc tgggcgggtc ccaggctatg gatgggagat
     2041 tctggatacg agtgcaggag tcagtgatgg tgccggaggg cctgtgcatc tctgtgccct
     2101 gctctttctc ctacccccga caagactgga cagggtctac cccagcttat ggctactggt
     2161 tcaaagcagt gactgagaca accaagggtg ctcctgtggc cacaaaccac cagagtcgag
     2221 aggtggaaat gagcacccgg ggccgattcc agctcactgg ggatcccgcc aaggggaact
     2281 gctccttggt gatcagagac gcgcagatgc aggatgagtc acagtacttc tttcgggtgg
     2341 agagaggaag ctatgtgaga tataatttca tgaacgatgg gttctttcta aaagtaacag
     2401 ccctgactca gaagcctgat gtctacatcc ccgagaccct ggagcccggg cagccggtga
     2461 cggtcatctg tgtgtttaac tgggcctttg aggaatgtcc acccccttct ttctcctgga
     2521 cgggggctgc cctctcctcc caaggaacca aaccaacgac ctcccacttc tcagtgctca
     2581 gcttcacgcc cagaccccag gaccacaaca ccgacctcac ctgccatgtg gacttctcca
     2641 gaaagggtgt gagcgcacag aggaccgtcc gactccgtgt ggcctatgcc cccagagacc
     2701 ttgttatcag catttcacgt gacaacacgc cagccctgga gccccagccc cagggaaatg
     2761 tcccatacct ggaagcccaa aaaggccagt tcctgcggct cctctgtgct gctgacagcc
     2821 agccccctgc cacactgagc tgggtcctgc agaacagagt cctctcctcg tcccatccct
     2881 ggggccctag acccctgggg ctggagctgc ccggggtgaa ggctggggat tcagggcgct
     2941 acacctgccg agcggagaac aggcttggct cccagcagcg agccctggac ctctctgtgc
     3001 agtatcctcc agagaacctg agagtgatgg tttcccaagc aaacaggaca gtcctggaaa
     3061 accttgggaa cggcacgtct ctcccagtac tggagggcca aagcctgtgc ctggtctgtg
     3121 tcacacacag cagcccccca gccaggctga gctggaccca gaggggacag gttctgagcc
     3181 cctcccagcc ctcagacccc ggggtcctgg agctgcctcg ggttcaagtg gagcacgaag
     3241 gagagttcac ctgccacgct cggcacccac tgggctccca gcacgtctct ctcagcctct
     3301 ccgtgcacta ctccccgaag ctgctgggcc cctcctgctc ctgggaggct gagggtctgc
     3361 actgcagctg ctcctcccag gccagcccgg ccccctctct gcgctggtgg cttggggagg
     3421 agctgctgga ggggaacagc agccaggact ccttcgaggt cacccccagc tcagccgggc
     3481 cctgggccaa cagctccctg agcctccatg gagggctcag ctccggcctc aggctccgct
     3541 gtgaggcctg gaacgtccat ggggcccaga gtggatccat cctgcagctg ccagataagc
     3601 tcgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccaggt aagccagccc
     3661 aggcctcgcc ctccagctca aggcgggaca ggtgccctag agtagcctgc atccagggac
     3721 aggccccagc cgggtgctga cacgtccacc tccatctctt cctcagcacc tgaactcctg
     3781 gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg
     3841 acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc
     3901 aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag
     3961 tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat
     4021 ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc
     4081 atctccaaag ccaaaggtgg gacccgtggg gtgcgagggc cacatggaca gaggccggct
     4141 cggcccaccc tctgccctga gagtgaccgc tgtaccaacc tctgtcccta cagggcagcc
     4201 ccgagaacca caggtgtaca ccctgccccc atcccgggag gagatgacca agaaccaggt
     4261 cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag
     4321 caatgggcag ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc
     4381 cttcttcctc tatagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt
     4441 ctcatgctcc gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct
     4501 gtccccgggt aaagcggccg ctacgtaaat tccgcccccc cccctccccc ccccctaacg
     4561 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4621 ccatattgcc gtcttttgca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4681 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4741 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4801 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4861 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4921 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4981 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5041 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     5101 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     5161 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5221 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5281 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     5341 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5401 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5461 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5521 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     5581 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5641 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5701 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5761 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5821 tggacgagct gtacaagtaa gtcgacgata aaataaaaga ttttatttag tctccagaaa
     5881 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     5941 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
     6001 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     6061 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     6121 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     6181 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     6241 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     6301 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
     6361 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     6421 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     6481 catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc
     6541 atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6601 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7441 catgagatta tcaaaaagga tcttcaccta gatccttttg cggccggccg caaatcaatc
     7501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttc
//
