LOCUS       sequence-358261-        9782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin 
            inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology
            arms for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT IAA17-3MYC
FEATURES             Location/Qualifiers
     source          1..9782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             440..1774
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     rep_origin      complement(1879..2334)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2016..2037)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2228..2247
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2460..2482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2474..2491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2475..2491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2501..2520
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2501..2519
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2544..2560
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2545..2561
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2655..3335
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="GSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNE
                     PANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGG
                     PEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNE
                     RKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEK
                     CKSRA"
     CDS             3363..3392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3399..3428
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3435..3464
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3947..4348
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4370..4390
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4928..4954
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4964..5020
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5057..5077
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5093..6814
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     primer_bind     complement(7009..7029)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7009..7027)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7048..7064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7048..7064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7061..7083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7072..7088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7096..7102,7103..7120,7121..7126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7141..7162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7279..7296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7450..8038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7530..7549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8209..9000,9001..9069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8832..8851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9070..9174)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    9211..9714
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9755..9773
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accacgcttt tcaattcaat tcatcaccat cctcatgaaa actgtgtaac ataataaccg
      241 aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct cgatgaaaaa aataagatat
      301 atataaggtt aagtaaagcg tctgttagaa aggaagtttt tcctttttct tgctctcttg
      361 tcttttcatc tactatttcc ttcgtgtaat acagggtcgt cagatacata gatacaattc
      421 tattaccccc atccatacaa tgccatctca tttcgatact gttcaactac acgccggcca
      481 agagaaccct ggtgacaatg ctcacagatc cagagctgta ccaatttacg ccaccacttc
      541 ttatgttttc gaaaactcta agcatggttc gcaattgttt ggtctagaag ttccaggtta
      601 cgtctattcc cgtttccaaa acccaaccag taatgttttg gaagaaagaa ttgctgcctt
      661 agaaggtggt gctgctgctt tggctgtttc ctccggtcaa gccgctcaaa cccttgccat
      721 ccaaggtttg gcacacactg gtgacaacat cgtttccact tcttacttat acggtggtac
      781 ttataaccag ttcaaaatct cgttcaaaag atttggtatc gaggctagat ttgttgaagg
      841 tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga accaaggctg tttatttgga
      901 aaccattggt aatccaaagt acaatgttcc ggattttgaa aaaattgttg caattgctca
      961 caaacacggt attccagttg tcgttgacaa cacatttggt gccggtggtt acttctgtca
     1021 gccaattaaa tacggtgctg atattgtaac acattctgct accaaatgga ttggtggtca
     1081 tggtactact atcggtggta ttattgttga ctctggtaag ttcccatgga aggactaccc
     1141 agaaaagttc cctcaattct ctcaacctgc cgaaggatat cacggtacta tctacaatga
     1201 agcctacggt aacttggcat acatcgttca tgttagaact gaactattaa gagatttggg
     1261 tccattgatg aacccatttg cctctttctt gctactacaa ggtgttgaaa cattatcttt
     1321 gagagctgaa agacacggtg aaaatgcatt gaagttagcc aaatggttag aacaatcccc
     1381 atacgtatct tgggtttcat accctggttt agcatctcat tctcatcatg aaaatgctaa
     1441 gaagtatcta tctaacggtt tcggtggtgt cttatctttc ggtgtaaaag acttaccaaa
     1501 tgccgacaag gaaactgacc cattcgaact ttctggtgct caagttgttg acaatttaaa
     1561 gcttgcctct aacttggcca atgttggtga tgccaagacc ttagtcattg ctccatactt
     1621 cactacccac aaacaattaa atgacaaaga aaagttggca tctggtgtta ccaaggactt
     1681 aattcgtgtc tctgttggta tcgaatttat tgatgacatt attgcagact tccagcaatc
     1741 ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc gtgatgagtt gtaaaattat
     1801 gtataaacct actttctctc acaagtactg cggtgtgaaa taccgcacag atgcgtaagg
     1861 agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1921 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1981 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2041 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2101 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2161 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2221 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2281 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc
     2341 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2401 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2461 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2521 cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     2581 cctgcagcta tattaccctg ttatccctac gtacgctgca ggtcgacggt ggcggttctg
     2641 gcggtggcgg atccggctct gttgagttaa acctgcgtga gaccgagcta tgtcttggtc
     2701 tacccggtgg tgacactgta gcaccagtga caggcaacaa aaggggcttc agcgagactg
     2761 tagatctgaa gctaaactta aacaacgaac cagctaataa ggaaggtagt acaacacatg
     2821 atgtggttac gtttgacagc aaggagaaga gtgcctgtcc caaagatccg gcaaagcccc
     2881 cggcgaaggc ccaggttgta ggttggcctc cggtaaggtc ctatcgtaaa aacgtaatgg
     2941 ttagttgtca gaagtccagc ggaggacctg aagcagcggc gttcgtcaaa gtatctatgg
     3001 atggcgcccc atacttgaga aagatagacc tgcgtatgta taagtcttac gacgagttat
     3061 caaatgcgct atctaacatg ttcagcagtt tcaccatggg caaacatggt ggtgaggagg
     3121 gaatgatcga cttcatgaac gagagaaaac ttatggacct ggtgaatagc tgggattatg
     3181 tccctagcta cgaggataaa gacggggatt ggatgcttgt gggggatgtt ccgtggccga
     3241 tgttcgtcga tacgtgtaag cgtctgcgtc taatgaaggg ctccgatgct attggtttag
     3301 ctccgcgtgc tatggaaaag tgtaagtcca gagctgtatc acccggggga tcctctagag
     3361 gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg
     3421 aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga tcctctagag
     3481 tcgactgact taagagttct tctttgagat atcgattgaa cgagctcgaa ttcatcgatc
     3541 tatattaccc tgttatccct agcgtaacta tgccaaatga caataagaca cccaataggt
     3601 ccagcactcc caagtttact aaaaaaccag taaccccgaa tgataaaatt cctgaaagac
     3661 gggtgtacaa tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat
     3721 aataccttcg ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag
     3781 ataccagaca agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg
     3841 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
     3901 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
     3961 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
     4021 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
     4081 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
     4141 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
     4201 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt
     4261 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt
     4321 tcaagctata ccaagcatac aatcaactat ctcatataca actagaatgc ccaagaagaa
     4381 acgtaaggtg ggttccaccc ttacgagcgg tcaacgtgac cacagagacg ttgaaaatga
     4441 gttatccagt gcagctttta gcagtcaaag tttcggggtt ccagatatgt cattcaaacc
     4501 ggggtgctcc agtgacgtcg cagtcactga tgcgggtatg cctagccaag ggttatggaa
     4561 caaccagacg caaaggatgc gtaccttcac taaggtccag aaaaggggct ctgttgggag
     4621 gagtatcgac atcaccaggt atcgtgatta cgatgagcta cgtcatgatc tggcatgtat
     4681 gttcggcata caggggcagt tagaagaccc ttatcgtatg gattggaagt tagtttatgt
     4741 ggatcacgag aacgatatac tgttggtggg cgacgacccg tgggaggaat ttgtaggttg
     4801 tgttaagtct atcaagattc tatctgctgc agaggtgcag caaatgagct tggacgggga
     4861 tcttggtgga gttccgcccc aaacccaggc gtgcagtgct tcagacgatg cgaacgcttg
     4921 gagaggctat ccctacgacg tgcctgatta tgcgggtagc ggggccacta acttcagctt
     4981 gttgaagcag gctggcgatg tggaggagaa tccaggtccg gcggctaccg ataaagcgga
     5041 attaattccc gagcctccaa aaaagaagag aaaggtcgaa ttgggtaccg ccacgtactt
     5101 cccggaggag gtggtggagc acatcttcag cttcctgccg gcgcagcgcg accgcaacac
     5161 ggtctcgctc gtctgcaagg tgtggtacga gatcgagagg ctgagccgcc gcggcgtctt
     5221 cgtgggcaac tgctacgccg tgcgcgccgg ccgcgtcgcc gcgcggttcc ccaacgtgcg
     5281 ggcgctcacg gtgaagggga agccccactt cgccgacttc aacctcgtgc cccccgactg
     5341 gggcggctac gcggggccgt ggatcgaggc ggccgcgagg ggatgccacg gcctggagga
     5401 gctcaggatg aagcggatgg tggtgtccga cgagagcctc gagctgctgg ctcgctcgtt
     5461 cccgcggttc agggctcttg ttcttatcag ctgcgagggg ttcagcactg acgggctagc
     5521 cgccgtcgcg agccattgca agcttctgag ggagttggat ttgcaggaaa atgaagtgga
     5581 ggatcgaggg cctaggtggc tttcctgctt ccctgattcc tgcacatcac ttgtctcatt
     5641 gaattttgcc tgcatcaaag gggaggttaa tgctggttca ctggagagac ttgttagcag
     5701 gtccccaaac ctgcggagtt tgaggctgaa tcgatctgta tcggtagata cacttgcaaa
     5761 gatactactg cgtaccccta acttggagga tttggggaca gggaatttga cagatgactt
     5821 ccaaactgag tcctacttta agcttaccag tgctctggag aaatgcaaga tgttgaggag
     5881 tttgtctgga ttctgggatg cttctcctgt ttgcctgtca tttatctacc ccctgtgtgc
     5941 tcaactgaca ggattgaact tgagctatgc acccacactt gatgcttctg accttacaaa
     6001 aatgattagc cgctgtgtga agctccaacg cctttgggta ctggattgta tctcggacaa
     6061 aggcttgcaa gtggtggcct ccagttgcaa agacttgcaa gaactcaggg tatttccatc
     6121 agatttctac gtagctggtt attctgcagt gacagaggag ggacttgttg cagtatcctt
     6181 gggctgtcca aaactgaact cactactgta cttctgtcac caaatgacta atgctgcact
     6241 agttactgtc gccaagaact gtccaaattt cacacgattc agactttgta ttcttgagcc
     6301 agggaagcct gatgttgtga caagccaacc attagatgaa ggctttggag ctattgttcg
     6361 tgagtgcaag ggattacaac gtttgtcaat atctggtctt ctcacagaca aagttttcat
     6421 gtatattggg aaatatgcaa aacaacttga gatgctttct atagcatttg ctggtgacag
     6481 tgataagggt atgatgcatg ttatgaatgg atgcaagaat ttaaggaaac tggagataag
     6541 agatagcccg tttggtgatg ctgcactctt ggggaatttt gctaggtacg agacaatgcg
     6601 atccctttgg atgtcatctt gcaatgtcac gttaaagggg tgccaagtcc ttgcgtcaaa
     6661 gatgccgatg ctcaatgttg aggtcataaa tgagcgggat ggtagcaatg aaatggagga
     6721 aaaccatgga gatctgccta aagtggagaa attatatgtg taccgcacaa ctgctggggc
     6781 gagggatgat gcaccaaatt ttgttaaaat cctagtcgac tccggttctg ctgctagtgg
     6841 ttgaacgcgt ggcgcctgga aaaagatttt caagtgaata aggagataaa accgtattga
     6901 ctttttatca ttctacaaaa gaccagtaac gaagtgtacc ctgctatatt accctgttat
     6961 ccctagcgta actcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg
     7021 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7081 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7141 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7201 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9181 tccccgaaaa gtgccacctg ggtccttttc atcacgtgct ataaaaataa ttataattta
     9241 aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa ttaaagaaaa
     9301 aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc
     9361 ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt
     9421 agaagaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat
     9481 atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga
     9541 aattttttaa acctttgttt attttttttt cttcattccg taactcttct accttcttta
     9601 tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca
     9661 aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcta
     9721 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9781 tc
//
