LOCUS       sequence-358267-        8402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MYC-tagged Acanthoeca spectabilis NF-kB2 protein in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Williams LM, Sridhar S, Samaroo J, Peart J, Adindu EK, Addanki A, 
            DiRusso CJ, Aguirre Carrion PJ, Rodriguez-Sastre N, Siggers T, 
            Gilmore TD
  TITLE     Comparison of NF-kappaB from the protists Capsaspora owczarzaki and 
            Acanthoeca spectabilis reveals extensive evolutionary 
            diversification of this transcription factor.
  JOURNAL   Commun Biol. 2021 Dec 16;4(1):1404. doi: 10.1038/s42003-021-02924-2.
  PUBMED    34916615
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-16"; volume: "4(1):1404. doi"; pages: " 
            10.1038/s42003-021-02924-2"
COMMENT     Sequence Label: pcDNA-MYC-As-NF-kB2
FEATURES             Location/Qualifiers
     source          1..8402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..32
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             42..71
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             81..110
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             159..188
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             222..251
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3059..3076)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3065..3289
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3335..3763
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3422..3441)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3632..3653
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3772..3792)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3777..4106
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3957..4092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4019..4038
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4173..4967
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4227..4246)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4837..4856
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5141..5262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5178..5197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5232..5251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5311..5327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5311..5327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5324..5346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5335..5351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5359..5365,5366..5383,5384..5389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5404..5425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5542..5559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5713..6301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5793..5812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6472..7263,7264..7332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7095..7114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7333..7437)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7512..7531)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7703..8082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8083..8286
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8237..8257
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8331..8350
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8331..8349
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
       61 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      121 agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag
      181 aggacttgaa tgaaatggag agcttgggcg acctcaccat ggagcaaaag ctcatttctg
      241 aagaggactt gaattctgag ccctggcgaa cacgccaata ctcacaaaaa cggtcaagaa
      301 ctgagagccc gcccaccggc atcgccatgt ctccacataa agtgctcggc ttcggcgggg
      361 cagccgcagc cgcagcagaa atggaagaag gtggcgctgc cgtaaccggc catgtgcctc
      421 tggggccgtt cggaggtatg caatccctga caatgcacca cttcgacttg ccggacttgt
      481 ctgatcctag gacgcctctg cggcttgatg acctcacgga aaaaccgcct ccaccgccgc
      541 acgatgccat tcctccctgg cctaatacat tcggagctgg gaaatcccct gttggcccct
      601 tgccaccccc tcataaacca acaaaggcag agcccatgtc tatcagtgaa ccgagttcac
      661 ctcgcaatga cgcaagcaga gtcgtggaga tgtcagctga aggctatcca atagtatctg
      721 gggttcttag aatcgtccag caacctgcgt ctcacgggcg gtttagatat tcaaaagaag
      781 gtaggaagac gccgctccat ggacgggagg acggcagcta tcccacggta gcaatcgccg
      841 ataggtacag gcacctggtg gaggaaggta cgcaagtaga tgtcaccctc gtaaccaaac
      901 ataacgacga acatggatct cccattcaac actggcatgt tcttgaagga aaagaaggtg
      961 gcccagtctc tcggcctctg aaggacggtg tcgcgacatt cccaaatctg gtggtaactc
     1021 gcaccactgc ggaaaaagag ggaggtgcgc gaaacgtaga ggatcagcat gttatcaggc
     1081 tgatgtatac aatgcggttt caggacgaga aaaggagaag cgtgctggcg agggcgataa
     1141 gtgaacccat ctttggtcaa gaggtcaaga tccaccgcat ttctcacatg cagattcctg
     1201 ccacgggaaa tatcgaagtc tttttcttga cctccaaaat taagcggaag aatactatat
     1261 tgactttcac tgagaccaca ccatcaagtt ttgatccggg gccacgatcc aagtggcagt
     1321 tggacaaaaa tatgtgtttg acatacgcca tgaaggattt gacggttcat tatcaatatg
     1381 ctgtagtggc tcgagtccct ccctattggg accagacgat cacctctccg cggaggatta
     1441 aagtgtgcct catagacacc gtccaaggcc tggaatcaaa tgtagttgaa attgattatg
     1501 tccctgctcc gttccgacca tactcttccg cgtctgctcc tcctggccac ccaccagtct
     1561 tcggcatggc accttctgtc catggatctt ggggagttcc tcaactcgat tggttcccgg
     1621 cggataatgc tgctggggcc gctgcagata tgcccatgag ggattccagt aattctccat
     1681 ctgtaagcgc gtcatccgaa tccacagata cacgaccttc tcgcgggagg gtatacgggt
     1741 ccttcgagac ttccgtttaa gaattccatg aataggcacc tctggaagag ccaactgtgt
     1801 gagatggtgc agcccagtgg tggcccggca gcagatcagg acgtactggg cgaagagtct
     1861 cctctgggga agccagccat gctgcacctg ccttcagaac agggcgctcc tgagaccctc
     1921 cagcgctgcc tggaggagaa tcaagagctc cgagatgcca tccggcagag caaccagatt
     1981 ctgcgggagc gctgcgagga gcttctgcat ttccaagcca gccagaggga ggagaaggag
     2041 ttcctcatgt gcaagttcca ggaggccagg aaactggtgg agagactcgg cctggagaag
     2101 ctcgatctga agaggcagaa ggagcaggct ctgcgggagg tggagcacct gaagagatgc
     2161 cagcagcaga tggctgagga caaggcctct gtgaaagccc aggtgacgtc cttgctcggg
     2221 gagctgcagg agagccagag tcgcttggag gctgccacta aggaatgcca ggctctggag
     2281 ggtcgggccc gggcggccag cgagcaggcg cggcagctgg agagtgagcg cgaggcgctg
     2341 cagcagcagc acagcgtgca ggtggaccag ctgcgcatgc agggccagag cgtggaggcc
     2401 gcgctccgca tggagcgcca ggccgcctcg gaggagaaga ggaagctggc ccagttgcag
     2461 gtggcctatc accagctctt ccaagaatac gacaaccaca tcaagagcag cgtggtgggc
     2521 agtgagcgga agcgaggaat gcagctggaa gatctcaaac agcagctcca gcaggccgag
     2581 gaggccctgg tggccaaaca ggaggtgatc gataagctga aggaggaggc cgagcagcac
     2641 aagattgtga tggagaccgt tccggtgctg aaggcccagg cggatatcta caaggcggac
     2701 ttccaggctg agaggcaggc ccgggagaag ctggccgaga agaaggagct cctgcaggag
     2761 cagctggagc agctgcagag ggagtacagc aaactgaagg ccagctgtca ggagtcggcc
     2821 aggatcgagg acatgaggaa gcggcatgtc gaggtctccc aggccccctt gccccccgcc
     2881 cctgcctacc tctcctctcc cctggccctg cccagccaga ggaggagccc ccccgaggag
     2941 ccacctgact tctgctgtcc caagtgccag tatcaggccc ctgatatgga caccctgcag
     3001 atacatgtca tggagtgcat tgagtagtct agagggcccg tttaaacccg ctgatcagcc
     3061 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3121 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3181 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3241 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3301 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3361 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3421 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3481 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3541 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3601 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3661 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3721 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     3781 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3841 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3901 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3961 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4021 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4081 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     4141 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4201 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4261 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4321 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4381 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4441 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4501 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4561 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4621 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4681 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4741 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4801 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4861 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4921 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4981 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5041 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5101 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5161 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5221 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5281 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5341 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5401 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5461 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5521 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5581 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5641 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5701 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5761 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5821 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5881 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5941 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6001 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6061 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6121 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6181 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6241 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6301 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6361 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6421 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6481 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6541 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6601 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6661 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6721 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6781 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6841 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6901 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6961 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7021 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7081 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7141 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7201 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7261 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7321 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7381 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7441 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7501 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     7561 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     7621 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     7681 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     7741 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     7801 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     7861 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7921 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     7981 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8041 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     8101 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     8161 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     8221 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     8281 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     8341 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc gatttaaagc
     8401 ta
//
