LOCUS       sequence-358272-        8278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression vector p17xQUAS-Luciferase, 
            alpha-cry:mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Hong J, Lee JG, Sohn KC, Lee K, Lee S, Lee J, Hong J, Choi D, Hong 
            Y, Jin HS, Choi DK, Lee SU, Kee Y, Jung J, Bae YK, Hwang RH, Hur GM,
            Lee JS, Ro H
  TITLE     IQ-Switch is a QF-based innocuous, silencing-free, and inducible 
            gene switch system in zebrafish.
  JOURNAL   Commun Biol. 2021 Dec 16;4(1):1405. doi: 10.1038/s42003-021-02923-3.
  PUBMED    34916605
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-12-16"; volume: "4(1):1405. doi"; pages: " 
            10.1038/s42003-021-02923-3"
COMMENT     Sequence Label: p17xQUAS-Luciferase, alpha-cry:mCherry
FEATURES             Location/Qualifiers
     source          1..8278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(659..670,671..808)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    820..840
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1260..1269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1266..2918
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1332..1350)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     2923..2939
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2924..2940
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4895..4904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4901..5611
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5046..5064)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5314..5333
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5734..5855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5771..5790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5825..5844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(5872..6059,6060..6071)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6090..6110)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6090..6108)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6129..6145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6129..6145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6142..6164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6153..6169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6177..6183,6184..6201,6202..6207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6222..6243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6360..6377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6531..7119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6611..6630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7290..8081,8082..8150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7913..7932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8151..8255)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccca
      661 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
      721 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
      781 tactccatta aaattgtact tgagtattct cgaggtcgac aactttgtat agaaaagttg
      841 gggtaatcgc ttatcctcgg ataaacaatt atcctcacgg gtaatcgctt atccgctcgg
      901 gtaatcgctt atcctcgggt aatcgcttat cctcgggtaa tcgcttatcc tcgggtaatc
      961 gcttatcctc ggataaacaa ttatcctcac gggtaatcgc ttatccgctc gggtaatcgc
     1021 ttatcctcgg ataaacaatt atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt
     1081 atcctcggat aaacaattat cctcacgggt aatcgcttat ccgctcgggt aatcgcttat
     1141 cctcgggtaa tcgcttatcc ttgcaagggt cgactctaga gggtatataa tggatcccat
     1201 cgcgtctcag cctcactttg agctcctcca cacgaattcc ctcgacctcg aagacgcgtg
     1261 ccaccatgga agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag
     1321 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa
     1381 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa
     1441 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
     1501 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg
     1561 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg
     1621 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg
     1681 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc
     1741 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat
     1801 acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct
     1861 ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat
     1921 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa
     1981 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg
     2041 gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg
     2101 attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca
     2161 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc
     2221 tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag
     2281 gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac
     2341 cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg
     2401 ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt
     2461 ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac
     2521 attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga
     2581 agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc
     2641 tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg
     2701 aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg
     2761 tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg
     2821 tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc
     2881 tcataaaggc caagaagggc ggaaagatcg ccgtgtaaga tctcgaggtc gacggtatcg
     2941 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagtaattaa gtctcagcca
     3001 ccgttaactg aacatgtcaa aacctgtgga gactgttgag atttgatgtt ctgaaaagat
     3061 aaagcctata aataaaatgt tgcccaaatt tcctgcctga tgtttttctt tgtctttgct
     3121 acatggcttt gctgctcgga tcggctcact ctgtgtatgc cacgttcact ttgtactctc
     3181 cttctcacgg taggtttatt atttttagat gtgcagttag tttctgtgaa ataacacacc
     3241 acacactgat attgtctgtg cattgacttg gtgagtgcac attgtttttg atcttgacat
     3301 atttatattt gattgatcag gtgaactgtg tgaatctaaa gtgctccata cagatgttct
     3361 gcattgaaaa tattctcatt ttattagtgg aagtgagtgt atgccacatc caatcaattt
     3421 cagcaaacac cccagtatga tttaatgcaa aaaaatgaag gtatcaaaca cgcattacta
     3481 ctttgcagtt aaatatttaa catttattcc aacacgaaaa aaagcagtaa ataacacttt
     3541 gacaaacacg tcaggacatc ttatttttgt caccctcaca ggcaatttag tataatatat
     3601 tatatatata tatatatcat ataataatat tcagtataat atatatatat atatcatatt
     3661 ataatattca gtataatata aaacacaaac acatatatgt ataatataat ataacatttt
     3721 tatttattga gatgcctcta tggaccgtgt tataagaagt aaagatcagg agaagtaaac
     3781 atgaagtgta attatgaata ctgatgttaa attaagctat gatgagtttt cactgttaat
     3841 ttaccatctc aattaaatgt tgatgcctcc atgaccaagt taagcagatg agactgagga
     3901 caactgtaga agacaagatg ttcactttgc tgaatatagc tggcttgaca gttatctatg
     3961 actctataaa tatatatata tttttttttt tataaaatga tttatttata actatatatc
     4021 catttctcag acaggtgctt catatccctc actcccgtag ctgtccatgc tggatctgtc
     4081 cccgttgttt ttaaaaagct aaataagtta ttaacatgac tgcatccagc gagccaaacc
     4141 tgtctggtgt acagctacca gagaagcttg agatcctagt caccggcggc cgctaatatg
     4201 gcaataaacg atcttcagag aaacttgcat tggtaaatag tttcagccta tgcatatgta
     4261 gttattcaaa ctgtaatgac tatcttacag tattatatgt ttataatagt ttgatccaat
     4321 aaatccagat ttatatgctt ttcaacataa aattgtgaaa tgcacagaat gtacatatac
     4381 aatacatttc ctacaataat gctctctttt ctattaatag tgtgcattca gtgcaggatg
     4441 ttatgtattt attttctgta gtgcaaactt tttcaaagaa ttttccagtc taagcttttc
     4501 agtcaagaaa aaaaaagcga ttgattatct gtgatattta aaaaaattag ctcttaaatc
     4561 tggtgaacca agtggccaca acacattaaa tactgacgtt ctacagccat gtagcttcat
     4621 ctggtctggt ttgttttggc aggcacttat tttccggtga tagtcggcca caagagtatt
     4681 gtctgagcca ttcagtgcta gactgtcatt ctcaggtcag aatccacccc actgattctg
     4741 ctgacaacac tgttcccacc atgagataat gccattccag agagatccat ttgtaagccc
     4801 ctctttctgc agcacaggta tataaccagg ggtctgcctc cactaaggcc ggcacacatc
     4861 atttggggat ctttgtactg tggatccacc ggtcgccacc atggtgagca agggcgagga
     4921 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     4981 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5041 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     5101 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     5161 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5221 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     5281 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     5341 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     5401 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     5461 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     5521 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     5581 caccggcggc atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     5641 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5701 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5821 agttgtggtt tgtccaaact catcaatgta tcttaagccg cggtggagct caagtgatct
     5881 ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt
     5941 ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa
     6001 agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact
     6061 ttacacctct ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     6121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
