LOCUS       sequence-358307-       10539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DCK* and TdTomato from the same transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10539)
  AUTHORS   Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, 
            Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, 
            Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi 
            SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
  TITLE     Targeting oncoproteins with a positive selection assay for protein 
            degraders.
  JOURNAL   Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 
            10.1126/sciadv.abd6263. Print 2021 Feb.
  PUBMED    33547076
REFERENCE   2  (bases 1 to 10539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-5"; volume: "7(6). pii: 7/6/eabd6263. doi"; pages: " 
            10.1126/sciadv.abd6263. Print 2021 Feb"
COMMENT     Sequence Label: pLX304-DCK*-IRES-Td
FEATURES             Location/Qualifiers
     source          1..10539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     204..223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     457..474
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    591..612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(627..632,633..650,651..657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    665..681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     670..692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     689..705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     689..705
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     724..744
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        726..744
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        772..998
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             999..1179
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1226..1351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1844..2077
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2262..2306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2481..2981
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2900..2918
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2993..3388
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3448..3565
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3682..3985
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3986..4189
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4140..4160
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4212..4236
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5020..5044)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5046..5087
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5146..5698
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5290..5307)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5517..5536
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5710..7140
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    7163..7751
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7216..7236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7634..7645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7823..8056
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8134..8255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8171..8190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8225..8244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8295..8430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8450..8469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8451..8469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8479..8496)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8479..8495)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8488..8510)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8637..9092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8724..8743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8934..8955
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9118..9222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9223..9291,9292..10083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9441..9460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10254..10539,1..303)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
       61 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      421 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      481 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      661 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
      721 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
      781 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
      841 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
      901 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
      961 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     1021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     1081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     1141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     1201 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     1261 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     1321 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     1381 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     1441 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     1501 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     1561 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     1621 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1681 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1741 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1801 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1861 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1921 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1981 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     2041 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     2101 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     2161 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     2221 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     2281 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     2341 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     2401 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     2461 tcccaacccc gaggggaccc ttgcgccttt tccaaggcag ccctgggttt gcgcagggac
     2521 gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat
     2581 tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg
     2641 cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga
     2701 cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag
     2761 caatggcagc gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg
     2821 ccgagagcag cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg
     2881 ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag
     2941 tcggctccct cgttgaccga atcaccgacc tctctcccca gggggtacca ccatggccaa
     3001 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     3061 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     3121 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     3181 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     3241 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     3301 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     3361 gctgccctct ggttatgtgt gggagggcct gcagctgcag tagtaagaat tctagatctt
     3421 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     3481 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     3541 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     3601 gcgccggctc gagggggccc ggggttatta atagtaatca attacggggt cattagttca
     3661 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     3721 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3781 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3841 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3901 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3961 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     4021 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4081 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4141 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     4201 tgtcgggatc aacaagtttg tacaaaaaag caggctccat ggccaccccg cccaagagaa
     4261 gctgcccatc tttctcagcc agctctgagg ggacccgcat caagaaaatc tccatcgaag
     4321 ggaacatcgc tgcagggaag tcaacatttg tgaatatcct taaacaattg tgtgaagatt
     4381 gggaagtggt tcctgaacct gttgccagat ggtgcaatgt tcaaagtact caagatgaat
     4441 ttgaggaact tacaatggag cagaaaaatg gtgggaatgt tcttcagatg atgtatgaga
     4501 aacctgaacg atggtctttt accttccaaa cctacgcctg tctcagtatg ataagagctc
     4561 agcttgcctc tctgaatggc aagctcaaag atgcagagaa acctgtatta ttttttgaac
     4621 gatctgtgta tagtgcgagg tatatttttg catctaattt gtatgaatct gaatgcatga
     4681 atgagacaga gtggacaatt tatcaagact ggcatgactg gatgaataac caatttggcc
     4741 aaagccttga attggatgga atcatttatc ttcaagccac tccagagaca tgcttacata
     4801 gaatatattt acggggaaga aatgaagagc aaggcattcc tcttgaatat ttagagaagc
     4861 ttcattataa acatgaaagc tggctcctgc ataggacact gaaaaccaac ttcgattatc
     4921 ttcaagaggt gcctatctta acactggatg ttaatgaaga ctttaaagac aaatatgaaa
     4981 gtctggttga aaaggtcaaa gagtttttga gtactttgaa cccagctttc ttgtacaaag
     5041 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     5101 gccgctacgt aaattccgcc cccccccccc cctccccccc ccctaacgtt actggccgaa
     5161 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     5221 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     5281 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     5341 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     5401 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     5461 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     5521 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     5581 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     5641 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     5701 gccacaacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg
     5761 cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     5821 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     5881 gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc
     5941 gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     6001 atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc
     6061 acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
     6121 cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg
     6181 ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag
     6241 ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac
     6301 accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc
     6361 tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg cagcggcagc
     6421 tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt catgcgcttc
     6481 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     6541 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     6601 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     6661 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     6721 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     6781 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     6841 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     6901 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     6961 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     7021 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     7081 gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct gtacaagtaa
     7141 accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     7201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7741 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7801 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7861 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7921 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7981 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8041 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     8101 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8221 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8281 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8341 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8401 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8461 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8521 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8581 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8641 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8701 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8761 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8821 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8881 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8941 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9001 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9061 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     9121 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9181 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9241 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9301 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9361 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9421 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9481 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9541 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9601 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9661 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9721 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9781 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9841 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9901 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9961 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10021 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10081 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10141 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10201 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10381 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10501 ggcgataagt cgtgtcttac cgggttggac tcaagacga
//
