LOCUS       sequence-358308-       14032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14032)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 14032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Autobow
FEATURES             Location/Qualifiers
     source          1..14032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..69,70..861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(219..238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1032..1620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1521..1540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1774..1791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6039..6063
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6112..6145)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6154..6187)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6196..6229)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     intron          7066..7131
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(7421..7440)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7486..7541
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7540..7559)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(7863..7896)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7906..7915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7912..7914,7915..7917,7918..8631)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8218..8237)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8565..8586
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8754..8875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8892..8925)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             8940..9644
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    9827..9948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9985..10018)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      10034..10043
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10040..10732
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    10760..10793
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(10832..10856)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(13663..13682)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13782..13804
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(13842..13860)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13928..14032
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1741 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1801 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcaggatc
     1861 gcggccgcga tccccgggcg agctcgaatt agttacgcta gggataacag ggtaatatag
     1921 aaaattcaga gaccgggaac caaactagcc tttaaaaaac ataagtacag gagccagcaa
     1981 gatggctcag tgggtaaagg tgcctaccag caagcctgac agcctgagtt cagtccccac
     2041 gaactacgtg gtaggagagg accaaccaac tctggaaatc tgttctgcaa acacatgctc
     2101 acacacacac acacaaatag tataaacaat tttaaatttc atttaaaaat aatttgtaaa
     2161 caaaatcatt agcacaggtt ttagaaagag cctcttggtg acatcaagtt gatgctgtag
     2221 atggggtatc attcctgagg acccaaaacc gggtctcagc ctttccccat tctgagagtt
     2281 ctctcttttc tcagccacta gctgaagagt agagtggctc agcactgggc tcttgagttc
     2341 ccaagtccta caactggtca gcctgactac taaccagcca tgaagaaaca aggagtggat
     2401 gggctgagtc tgctgggatg ggagtggagt tagtaagtgg ccatggatgt aatgacccca
     2461 gcaatgctgg ctagaaggca tgcctccttt ccttgtctgg agacggaacg ggagggatca
     2521 tcttgtactc acagaaggga gaacattcta gctggttggg ccaaaatgtg caagttcacc
     2581 tggaggtggt ggtgcatgct tttaactcca gtactcagga ggcagggcca ggtggatctc
     2641 tgtgagttca agaccagcct gcactatgga gagagttttg ggacagccag agttacacag
     2701 aaaaatcctg gtggaaaatc tgaaagaaag agagaaagaa agaaagaaag aaaggaagaa
     2761 agaaagaaag agtggcaggc aggcaggcag gaggaaggaa ggaaggaagg aaggaaggaa
     2821 ggaaggaagg aaggaaaata ggtgcgactt caagatccgg agttacaagc agaatgcact
     2881 gtttccctaa cagggccaag tgttttgagt aactgaaggt gggcatgatg cctgggaagc
     2941 agaaacaagc caggcagatg caccccttgc cttgcttccg aagggctgca gtagcatgga
     3001 aaacatggaa aacaaccaat ccattccctt tgctgatata acaggctcca aagccaaaac
     3061 ctgtcactgg aggctcaaga gcagatctcc agccaagagg caaaggaatg ggggaagctg
     3121 gagggcctcc ctctggttat ccaggcttct gaaggttcaa gcaaagaaag ggttacaacc
     3181 ttaaaaggag agcgtcccgg ggtatgggta gaagactgct ccaccccgac ccccagggtc
     3241 cctaaccgtc ttttccctgg gcgagtcagc ccaatcacag gactgagagt gcctctttag
     3301 tagcagcaag ccacttcgga cacccaaatg gaacacctcc agtcagccct cgccgaccac
     3361 cccaccccct ccatcctttt ccctcagcct ccgattggct gaatctagag tccctccctg
     3421 ctcccccctc tctccccacc cctggtgaaa actgcgggct tcagcgctgg gtgcagcaac
     3481 tggaggcgtt ggcgcaccag gaggaggctg cagctagggg agtccaggtg agagcaggcc
     3541 gacgggaggg acccgcacat gcaaggaccg ccgcagggcg aggatgcaag ccttccccag
     3601 ctacagtttt gggaaaggat accagggcgc tcctatatgg gggcgcggga actggggaaa
     3661 gaaggtgctc ccaggtcgag gtgggagagg aaggcagtgc ggggtcacgg gctttctccc
     3721 tgctaacgga cgctttcgaa gagtgggtgc cggaggagaa ccatgaggaa ggacatcaag
     3781 gacagccttt ggtccccaag ctcaaatcgc tttagtggtg cgaatagagg gaggaggtgg
     3841 gtggcaaact ggagggagtc cccagcgggt gacctcgtgg ctggctgggt gcggggcacc
     3901 gcaggtaaga aaaccgcaat gttgcgggag gggactgggt ggcaggcgcg ggggagggga
     3961 aagctagaaa ggatgcgagg gagcggaggg gggagggagc gggagaatct caactggtag
     4021 aggaagatta aaatgaggaa atagcatcag ggtggggtta gccaagccgg gcctcaggga
     4081 aagggcgcaa agtttgtctg ggtgtgggct taggtgggct gggtatgaga ttcggggcgc
     4141 cgaaaacact gctgcgcctc tgccaaatca cgctacccct gtatctagtt ctgccaggct
     4201 tctccagccc cagccccaat tcttttctct agtgttcccc cttccctccc ctgaatctca
     4261 agcccacact ccctcctcca taacccactg ttatcaaatc taagtcattt gccacccaac
     4321 aaccatcagg aggcggaagc agacgggagg agtttgagat caacttgggc tacatcacga
     4381 gttccaggct caccaaggct tcttaaggag accttgtctc taaaattaat taattaatta
     4441 attaatagtc ccctttctct gccacagaac cttgggatct ggctcctggt cgcagctccc
     4501 cccaccccag gctgacattc actgccatag cccatccgga aatcctagtc tatttcccca
     4561 tggatcttga actgcagaga gaatggcaga gtggcccgcc ctgtgcaaag gatgttccta
     4621 gcctaggtgg agctcgcgaa ctcgcagact gtgcctctct tgggcaagga caggctagac
     4681 agcctgccgg tgtgttgagc tagggcactg tggggaaggc agagaacctg tgcagggcag
     4741 caatgaacac aggaccagaa aactgcagcc ctaggaacac tcaagagctg gccatttgca
     4801 agcatctctg gcctccgtgc ttctcactca tgtcccatgt cttatacagg cctctgtggc
     4861 acctcgcttg cctgatctca tccctagccg ttaagctttc tgcatgactt atcacttggg
     4921 gcataatgct ggatacctac cattttctta gaccccatca aaatcctatt tgagtgtacg
     4981 gttcggagaa cctcatttat ccggtaaatg tcttttactc tgctctcagg gagctgaggc
     5041 aggacatcct gagatacatt gggagaggag atacagtttc aataaaataa taggttgggt
     5101 ggaggtacat gcctataatg ccaccactca ggaaatggtg gcagcttcgt gagtttgagg
     5161 ccaacccaag aaacatagtg aaaccctgtc agtaaataag taagcaagta tttgagtatc
     5221 tactatatgc tagggctgac ctggacatta ggggtcatct tctgaacaaa ctagtgcttg
     5281 agggaggtat ttggggtttt tgtttgttta atggatctga atgagttcca gagactggct
     5341 acacagcgat atgactgagc ttaacacccc taaagcatac agtcagacca attagacaat
     5401 aaaaggtatg tatagcttac caaataaaaa aattgtattt tcaagagagt gtctgtctgt
     5461 gtagccctgg ctgttcttga actcactctg tagaccaggc tggcctggaa atccatctgc
     5521 ctgcctctgc ctctctgcct ctctgcctct ctgcctctct ctctgcctct ctctgcctct
     5581 ctctgcccct ctctgcccct ctctgcccct ctctgccgcc ctctgccttt tgccctctgc
     5641 cctctgttct ctggcctctg ccctctgccc tctggcctct ggcctctgcc tctgcctctt
     5701 gagtgctgga atcaaaggtg tgagctctgt aggtcttaag ttccagaaga aagtaatgaa
     5761 gtcacccagc agggaggtgc tcagggacag cacagacaca cacccaggac ataggctccc
     5821 acttccttgg ctttctctga gtggcaaagg accttaggca gtgtcactcc ctaagagaag
     5881 gggataaaga gaggggctga ggtattcatc atgtgctccg tggatctcaa gccctcaagg
     5941 taaatgggga cccacctgtc ctaccagctg gctgacctgt agctttcccc accacagaat
     6001 ccaagtcgga actcttggca cctagaggat ctcgaatcac aagtttgtac aaaaaagcag
     6061 gctttaaagg aacgcacgta actataacgg tcctaaggta gcgaacctag gataacttcg
     6121 tatagcatac attatacgaa gttatcggcg cgcataactt cgtataggat actttatacg
     6181 aagttatcaa gcgctataac ttcgtatagt ataccttata cgaagttatc tgatgcaatg
     6241 gccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     6301 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     6361 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     6421 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     6481 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     6541 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     6601 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     6661 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     6721 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     6781 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     6841 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     6901 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     6961 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     7021 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggtaaa tataaaattt
     7081 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta ggatgactct
     7141 ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc gcgagatatg
     7201 gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac caatgtaaat
     7261 attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt gcgcctgctg
     7321 gaagatggcg attaagcggc cgcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     7381 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     7441 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     7501 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     7561 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     7621 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     7681 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     7741 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     7801 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcgcta
     7861 gcataacttc gtatagcata cattatacga agttatctga acgttgccac catggtgagc
     7921 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     7981 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     8041 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     8101 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     8161 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     8221 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     8281 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     8341 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     8401 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     8461 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     8521 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     8581 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     8641 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     8701 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     8761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     8881 tgtttaaaca tataacttcg tataggatac tttatacgaa gttatcgcgt acggccacca
     8941 tgagcagcgg cgccctgctg ttccacggca agatccccta cgtggtggag atggagggca
     9001 atgtggatgg ccacaccttc agcatccgcg gcaagggcta cggcgatgcc agcgtgggca
     9061 aggtggatgc ccagttcatc tgcaccaccg gcgatgtgcc cgtgccctgg agcaccctgg
     9121 tgaccaccct gacctacggc gcccagtgct tcgccaagta cggccccgag ctgaaggatt
     9181 tctacaagag ctgcatgccc gatggctacg tgcaggagcg caccatcacc ttcgagggtg
     9241 atggcaattt caagacccgc gccgaggtga ccttcgagaa tggcagcgtg tacaatcgcg
     9301 tgaagctgaa tggccagggc ttcaagaagg atggccacgt gctgggcaag aatctggagt
     9361 tcaatttcac cccccactgc ctgtacatct ggggcgatca ggccaatcac ggcctgaaga
     9421 gcgccttcaa gatctgccac gagatcaccg gcagcaaggg cgatttcatc gtggccgatc
     9481 acacccagat gaataccccc atcggcggcg gccccgtgca cgtgcccgag taccaccaca
     9541 tgagctacca cgtgaagctg agcaaggatg tgaccgatca ccgcgataat atgagcctga
     9601 aggagaccgt gcgcgccgtg gattgccgca agacctacct gtgaagctta attagctgag
     9661 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctggc cgcgactcta
     9721 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     9781 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     9841 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9901 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtgaagag
     9961 cttcataatc ttcgcgttct tcatataact tcgtatagta taccttatac gaagttatca
    10021 agtatccagt actgccacca tggtgagcga gctgattaag gagaacatgc acatgaagct
    10081 gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
    10141 gccctacgag ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt
    10201 cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac
    10261 ccagggcatc cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt
    10321 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
    10381 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat
    10441 gcagaagaaa acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg
    10501 cctggaaggc agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa
    10561 cttgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta
    10621 ctatgtggac agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca
    10681 gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
    10741 ttaagcggcc gcaactagtg aagttcctat tctctagaaa gtataggaac ttccgtaact
    10801 ataacggtcc taaggtagcg aatgcatcta gacccagctt tcttgtacaa agtggtgatt
    10861 cgaggtcctt cctctgcaga ggtcttgctt ctcccggtca gctgactccc tccccaagtc
    10921 cttcaaatat ctcagaacat ggggagaaac ggggaccttg tccctcctaa ggaaccccag
    10981 tgctgcatgc catcatcccc cccaccctcg cccccacccc cgccacttct ccctccatgc
    11041 ataccactag ctgtcatttt gtactctgta tttattccag ggctgcttct gattatttag
    11101 tttgttcttt ccctggagac ctgttagaac ataagggcgt atggtgggta ggggaggcag
    11161 gatatcagtc cctggggcga gttcctccct gccaaccaag ccagatgcct gaaagagata
    11221 tggatgaggg aagttggact gtgcctgtac ctggtacagt catactctgt tgaaagaatc
    11281 atcggggagg ggggggggct caagagggga gagctctgct gagcctttgt ggaccatcca
    11341 atgaggatga gggcttagat tctaccaggt cattctcagc caccacacac aagcgctctg
    11401 ccatcactga agaagccccc tagggctctt gggccagggc acactcagta aagatgcagg
    11461 ttcagtcagg gaatgatggg gaaaggggta ggaggtgggg gagggatcac cccctcctct
    11521 aaaacacgag cctgctgtct ccaaaggcct ctgcctgtag tgagggtggc agaagaagac
    11581 aaggagccag aactctgact ccaggatcta agtccgtgca ggaaggggat cctagaacca
    11641 tctggttgga cccagcttac caagggagag cctttattct tctttccctt gcccctctgt
    11701 gccagcccct cttgctgtcc ctgatccccc agacagcgag agtcttgcaa cctgcctctt
    11761 ccaagacctc ctaatctcag gggcaggcgg tggagtgaga tccggcgtgc acactttttg
    11821 gaagatagct ttcccaagga tcctctcccc cactggcagc tctgcctgtc ccatcaccat
    11881 gtataatacc accactgcta cagcatctca ccgaggaaag aaaactgcac aataaaacca
    11941 agcctctgga gtgtgtcctg gtgtctgtct cttctgtgtc ctggcgtctg tctcttctgt
    12001 gttcttccaa ggtcagaaac aaaaaccaca cacttcaacc tggatggctc ggctgagcac
    12061 ttctgtgtgc agaaggtcca accagactct ggggtacccc ggccctccct attcccttgc
    12121 ctcctgtctc ccgcttttta tagctcccta tgctgggctt ctctggagag tgaaatcttt
    12181 gcccaaatca atgcgcattc tctctgctga gtcatctggc gacagcagtt gagttcaccc
    12241 gccaacacat gggcccagct atgtagccga accctggctc tggaagtgcc agggactttg
    12301 tgcataagta tgtaccatgc ccttttttca cagtcctagc tctgcagaag tgcagcctga
    12361 aggcctgtct gctgagagga catgccctgg agccctgaaa caggcacagt gggaggagga
    12421 acggaggatg acaggcatca ggccctcagt ccaaaagcaa ccacttgaga atgggctgga
    12481 gtacgaaaca tggggtcccg tccctggatc cctcctcaaa gagtaataag taaaatataa
    12541 acaggtaccc caggccgttc tgggtttggg ttgtaatggg atccatttgc agagaactat
    12601 tgagacagcc cagccgtact gtgacaggca atgtggggga ggaggttgaa tcacttggta
    12661 tttagcatga atagaataat tccctgaaca tttttcttaa acatccatat ctaaattacc
    12721 accactcgct cccagtcttc ctgcctttgc gccagcctcc tgtctggcca tgcctgaaga
    12781 aggctggaga agccacccac ctcaggccat gacactgcca gccacttggc aggtgcagcc
    12841 aaacctgagc tgtcccagaa agggacattc tcaagaccca ggcaccctga tcagcactga
    12901 cttggagcta caagtgtcat gccagaaaag tctctaagaa aaccttttca gggaaaaggg
    12961 ggtgactcaa caccgggcaa gtttgggaag ccccaccctt cgagtgatgg aagagcagat
    13021 aggaagcctc agaagagaga caccggcacc caggtaacgt tcctcatgtg gtctctgtca
    13081 cacggtcgac tctcttaagg tagctcgacc gtgctcttcc ctggacatct ccgtgaccac
    13141 actctcagtt cttagggaga tgcgggtgct ctctgaggct atctcagagt tgcagattct
    13201 gaggcctaga gtgactacag tcagcctagg aagccacaga ggactgtgga ccaggagggc
    13261 agaagaggag aagggaagaa aaaccatcag ataggacttg caatgaaact aacccaagac
    13321 aatcataatg cagacaggaa tgttaaaggc gttcagcagc tggccatgac acccatctgt
    13381 ccctctggcc aagtcagcaa gcctggaaga cctgggactc ctgcccatat gtcctaagct
    13441 ccccacccac ccactcgttc actgtcctta ttctctctct accttcagcc acttagtttc
    13501 ctaccttaag tcctagaatt gatcctggcg taatagcgaa gaggcccgca ccgatcgccc
    13561 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
    13621 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
    13681 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    13741 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    13801 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
    13861 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
    13921 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    13981 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gt
//
