LOCUS       sequence-358315-       11385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Frameshift reporter (control plasmid for screening).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity 
            AJ, Zhang F, Blainey PC
  TITLE     Optical Pooled Screens in Human Cells.
  JOURNAL   Cell. 2019 Oct 17;179(3):787-799.e17. doi: 
            10.1016/j.cell.2019.09.016.
  PUBMED    31626775
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "787-799.e17"
COMMENT     Sequence Label: pL_FR_Hygro
FEATURES             Location/Qualifiers
     source          1..11385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2450..3627
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2681..3618
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3575..3595
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3668..3677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3823..3876
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4267..4293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4313..4369
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4760..4789
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4805..4858
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4859..5875
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK"
     misc_feature    5912..6500
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5965..5985)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6383..6394)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6503..6519)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6504..6520)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7025..7205
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7231..7248)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7237..7461
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7507..7935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7594..7613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7804..7825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7944..7964)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7988..8198
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8010..8206
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8057..8192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8119..8138
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8254..8301
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8320..8694
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8824..8945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8861..8880)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8915..8934
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8994..9010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8994..9010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9007..9029)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9018..9034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9042..9048,9049..9066,9067..9072))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9087..9108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9225..9242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9396..9984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9476..9495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10155..10946,10947..11015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10778..10797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11016..11120)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11195..11214)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct gcaaagatgg ataaagtttt aaacagagag
     2401 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     2461 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2521 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2581 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2641 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     2701 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     2761 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     2821 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     2881 gggccaccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     2941 tctagccatt taaaattttt gatgacctgc tgcgacgctt ttttctggca agatagtctt
     3001 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     3061 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     3121 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     3181 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     3241 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     3301 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     3361 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     3421 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     3481 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     3541 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     3601 ttttttcttc catttcaggt gtcgtgaacc ggttaatagt gagtagtagt aagtgataat
     3661 agaaatcgcc accatggccc tctcggcgca tgtgtggggg cccgaagaac gccatgtcca
     3721 gcgtgggcgg agactgcaaa tagctgcctg gcgcgcattt gggatagaga tctgtggcac
     3781 cttccataca gtagtggcgg cagatcccgt acaggcagtg gagagggcag aggaagtctg
     3841 ctcacctgcg gcgacgtcga ggagaatcct ggcccaccag agccagcgaa gtctgctccc
     3901 gccccgaaaa agggctccaa gaaggcggtc accaaggcgc agaagaaagg cggcaagaag
     3961 cgcaagcgca gccgcaagga gagctattcc atctacgtat acaaggttct caagcaggtc
     4021 caccccgaca ccggcatttc gtccaaggcc atgggcatca tcaactcgtt tgtcaacgac
     4081 attttcgagc gcatcgcagg cgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
     4141 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
     4201 cacgccgtgt ccgagggcac caaggccatc accaagtaca ccagcgccaa aggaggaggc
     4261 ggaagctacc catacgacgt tccagattac gcttaataat aacggatccg gcgcaacaaa
     4321 cttctctctg ctcaaacaag ccggcgacgt cgaagagaat cctgggccgc ctgagcccgc
     4381 taaaagtgca cctgcgccaa agaaaggatc taagaaagcg gtaaccaaag cccaaaagaa
     4441 gggcggaaag aagcgaaaac gaagtcggaa agagtcatat tctatttacg tctacaaagt
     4501 gctgaaacaa gttcacccag atacggggat aagctcaaag gcaatgggaa ttatgaacag
     4561 ctttgtaaac gatatttttg agcggattgc cggcgaggcg agtaggcttg ctcattacaa
     4621 caagcgatcc acgattacat ctagggaaat ccagacagcg gttcggctgc tcctccctgg
     4681 agaactggca aaacacgccg tttctgaggg caccaaagca atcacgaaat atacaagcgc
     4741 gaagggtggt ggaggaagtg aacaaaaact catctcagaa gaggatctgc gtacgggcag
     4801 tggggagggc agaggaagtc tgctaacctg cggtgacgtc gaggagaatc ctggcccaaa
     4861 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     4921 gtccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     4981 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     5041 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     5101 gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     5161 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     5221 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     5281 tcaatacact acgtggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     5341 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     5401 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     5461 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     5521 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     5581 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     5641 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     5701 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     5761 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     5821 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggatct
     5881 gcagtaagaa ttcgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga
     5941 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6001 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6061 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6121 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6181 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6241 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6301 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6361 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6421 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6481 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     6541 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     6601 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     6661 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6721 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     6781 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     6841 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     6901 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     6961 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     7021 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7081 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7141 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7201 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     7261 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     7321 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     7381 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     7441 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     7501 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7681 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7801 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7861 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7921 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7981 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8041 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8101 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8161 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8221 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     8281 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8341 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     8401 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     8461 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     8521 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     8581 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     8641 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     8701 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8761 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8821 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8881 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8941 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9001 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9061 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9181 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9241 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9301 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9361 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9421 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9481 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9541 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9601 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9661 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9721 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9781 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9841 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9901 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9961 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10021 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10081 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10141 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10201 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10261 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10321 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10381 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10441 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10501 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10561 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10621 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10681 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10741 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10801 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10861 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10921 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10981 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11041 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11101 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    11161 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    11221 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
    11281 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
    11341 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
