LOCUS       sequence-358319-       12339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHelz-E958Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 12339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmHelz-E958Q_AH
FEATURES             Location/Qualifiers
     source          1..12339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2630..2651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2891..2910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3238..3259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9645..9686
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9696..9713
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9736..9753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9786..9920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9810..9829)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9864..9883
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(10521..11109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10601..10620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11280..12071,12072..12140))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11903..11922
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12141..12245)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12313..12331
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 catcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2641 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2701 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2761 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2821 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2881 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2941 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3001 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3061 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3181 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3301 gccaagcttg atggccgccg agaaggagat gcaggcccac gacatggtcc gacgacggag
     3361 ttgggcccgt gctgtggcgc tctacgacca gctgattgcg cccagtcccg gccagggaag
     3421 cggtggccag agtgcaggat caagggccca gaaggaccag cagatcgcct gcctactggg
     3481 acgatgcgag tgcctcctag agctgggaaa gttcgagggt tgtctggcgg acgcctacaa
     3541 ggtgctcacg ctgctctccg agcaaacaga gtgcctggcc agcgtgtcca gggcacgccg
     3601 atggctcgtc cacgctcttt tcaagatgca caagtacggg gatgccgaaa tcttcctgag
     3661 caagtggatc aacgagctga gcggccagca gatatactcg gacatatcga aaacccttga
     3721 gcggtacaag tccatcattc agatgatcat gaacgggcag cacaaggcgc aggccctgaa
     3781 gatcccacac gcccgtctcg aggacgagat ggctatactg gataccaaac tggatcactg
     3841 ggccaccaac aacctgccgc tggacaagta cagcaagctg ctcagcaaca agggcttgaa
     3901 gaagcgggag cagcagcagc aacagcagca gcaacaacag cagcagaatg gcaatggaat
     3961 cggcagttat gcgagcagca gcagtagctc ctcaactggc agcagcagca cgatgtccag
     4021 ttccagcaca agtctccaca agaccaacga tctgctggcg gccatgaagc tcacggcagg
     4081 aaaacaccag ccgggctccg gcgacggatc tggctcggat cagggcgatc aggcggctcc
     4141 atcgaccacc tgcagctact gcaccatcac ctttcagacg aggaacgaac tgcgacagca
     4201 ctgccagacc gaggcgcacc agaaggtgat catgagcgat gagggtcgtg actggaagtg
     4261 gcgtccgccg ccccgtggtt atacactgga cacctattcc ctgtgcgaga cgttcagcga
     4321 ggcgcacatc tgtcattatg gagcccagtg cgtggaggcc catggacagg atgagttgaa
     4381 cgagtggaag gagcgcttcg agtaccggcg aatgcggatg cagaaggctt gtgagaagga
     4441 gctctacggc aagtcgtaca cggagcaagt gctggatcgt tggattcagg cgacagcacc
     4501 ggagcgggtg atgtgcgagc gagtgccgga catggaggca cgctgtgagc agcagctggt
     4561 gactagcatt agctcaaaga cctccaagcg ggagtggctc ttccaattag agacgaagaa
     4621 gccgctaaaa gcggtagccc tcttgcagga tgcccatcga aatcactttg ccctgaagtc
     4681 cataaaaatg tgcaagccaa cggagtccag catggaactc aaatcagacc aagagtggct
     4741 ggcaggcagt tcatcgcaat cggagacttc caacgaggac tctgctactt tgattcacga
     4801 aatcacagtt gagtttcaca cggagatcta cggcaccttt cggcaagcca tctgcttcga
     4861 tgtgggtcag gagcctttgc ttgtgaggca tctgtgcgtg gatgtgttgc ccgtgaacga
     4921 tgcggagaaa atcgaggaga tcaagcggga catcattaac agttctgcca cccgatggga
     4981 tgtcgccaat acgcagctca cacgcttcga aacgacgatt ggtactcatc tcaaaacgga
     5041 agcgtatctc agcgatttgg aacacgaaag ggagctattg gagaggtatc cctgtcccag
     5101 ggcagccact ttcactctga cccagtcgac cattgtggag aagcggttga cgcagaataa
     5161 ctaccgctca cgcatccacg agttgttgtc agtggaggag attgcccgct atgagcagat
     5221 agcgaggtat aatgtaagga ctcgtctcac agtggcatcg aattacattc ttacccccgc
     5281 gggaatggcc accagtacgg ccaagtattc cctggcaggt gaactgtttg ctcttatgcg
     5341 tttgggcaag gacatttcgg aggatacgtc tcctggcaga ttaattctct cgaactgcag
     5401 cagtgtgtat atctcaaaac ctgaggatcc cgattcgaaa gcagacccaa ctaaacgtgc
     5461 agtttacgaa gcgttgatcg aggacaaagg caagaatgtg atttacttga aactatccgc
     5521 aaaatgtgtg gaggcaatgg ctctgcaggc ggataccgaa ctggaggtgg acatacaatt
     5581 ccagctgaac cgaatgccat actgtgagtg gcacaatgcg gtggataaga tcacagattt
     5641 ccggttgatc tttccggcta cggaactgga accgagtata ccgtggacac cgaagaagca
     5701 gtgggccgat tcctgtgaac cgaagttgaa tgccaaacaa agggaggccg tgaatgccat
     5761 taccacagct ctgagcatta agttgccgcc aatcctgctg ataggaccct ttggcactgg
     5821 aaaaacctac acgctggccc aagctatcaa acaactgctc acgcagccgg aggccaaaat
     5881 tctgatctgc acgcactcga attcagccgc tgacttgtac atcaaggaat acctgcatcc
     5941 gtggatcgag gagggtttga aggaggccac accgttgcga gtttactacc ataagagatg
     6001 gtcagccacg gtaaacggtg tggtgcaaaa atactgcatc accgatggag ttggcaactt
     6061 cctaaggccc actgtggagg atataatgcg tcatagaatt gtagttgtta cgctgagtat
     6121 cagcatggag ttggccactt tgggtctgcc caagggattg ttcactcaca tctttctgga
     6181 ccaagctgct caggcaatgg aatgcgaggc cattatgccg cttgctttgg ccaatgattc
     6241 tacaaggatc gttttggctg gagatcacat gcaaatgagt ccggaactgt tctcagcctt
     6301 tgccaaggaa cgcaagctgc acatttcgct gttggagcgg ctgtacgacc actatccctc
     6361 caacttcccc tgcaagatcc tgctgtgcga gaactaccgt gcccacgagg ccatcatccg
     6421 tttcacttcg gagcttttct acgaacaaaa gctggtggcc tccggaaaac agccacggca
     6481 cgatcgcttt tatcccttga ccttcttcac tactcgggga gaggatgttc aggataaaaa
     6541 ctcaactgct ttctataata atgctgaagt ttatgaggtg gtggaaaggg tttccgagct
     6601 gcggaaacgt tggcccagtg cttggggtaa actgaatgat accagcattg gcattatgac
     6661 tccctactcg gatcaggtgt ttcgcattcg ttccgagctg agaaaacggc gcatgggtgg
     6721 aatatccgta gaacgtgtgc tcaatgtcca gggtaaacag tttcgggcgg tattcctctc
     6781 tacagttcga acccgacgca cctgcatgcc ccaaggaagt gcccctggtt ccgcttccac
     6841 aaccagcatt ggcgatgccg acgcagacta tggattcttg agcaattcca agctgctgaa
     6901 cacggctatt actcgtgccc agtcgttggt tgcagtggtt ggtgatcccg tggctctttg
     6961 ttccattggt cgctgccgga aagtgtggga gcgtttcatc gaaatctgcg acgaacataa
     7021 aagtcttttt ggcttaactt ggtcgaacct gcgctcccaa ctggatggcg tggagcttaa
     7081 gcgtggctat gtgctaaatc ctctggctcc cgagttcgtc ccgcgtgccc ttcaaccgga
     7141 ggcatatctg cgggagcagg ctgctctcta tctaaatggt ccgcaacacg gacacggcgg
     7201 ccatggaccg ccgggtcctc cggctcattt tgccaacgca gctggaatgc tgggtcccgg
     7261 acccgcggcc gctgctcacc agatgaatca aatggctgct gccatggctg ccaatcagca
     7321 acagctcaca gcccacatgt actccaccca catggccatg atggccgctg ccgtcggagg
     7381 aaagtctgga aatggccctg gaccaggaac cggcggagga cccggtccta gtccaggccc
     7441 acctccacac tacggcggcg gcggtggtgg tggcggacac atgggaatga gaggaccacc
     7501 gccaccggcg ccgcatctgc gtggaatgcc acccggtgga ccgccaccca cacagcagcc
     7561 aggcggagct ggtggcggcg ggggtccacc gccaccatac ggacaatatc cagccatgca
     7621 gcactggcga ccaccgcagc agggacagaa tcaaggccca ggacagcagc caccacaaaa
     7681 tccgaatgcc agtctctggg gtccgccgcc gcaaacgaat ccgtggagtg tgctgccgcc
     7741 cagtaagcag ccacagcaac ctcagccacc gccagttagc gcgcccggac gtggaccggg
     7801 tcctttgcca ccgccactca atgcaaaccc gtctcttcca ctgagaggtg gtagtgtgcc
     7861 gccaccgccg ccgaatgcct cgactgctgc tcacctgcta aggaatccca gcgaactggg
     7921 ctacttggcc aagaagtcgc tgtacaacca tggccaggca ttgccgcatc atcagcttta
     7981 tggacccgga ccaggaccac cgccgcctcc atcgcagcaa cagccgcagg caccgccgat
     8041 gggtatgcag aggggcagca gtgtgccgaa cggacccatg tcgcccatgg agaacggacc
     8101 gccgccacct ccctatctga ggcacaatgg tagtggctac aatccgggag caccgccacc
     8161 gcctccgcag caacaaccac agccaccacc caatccgttt atgtacgccg gcaagcaacc
     8221 gtctccgagc tctatgagat ttggaccaat gacgcacgag ctactcccac ccaatgtaag
     8281 catctatgac atggctttta atccgaaaga cgagcaatac aagtggtacg ttaaactgct
     8341 tgaaaccgtg ggtcaggatg gtgccaacaa gtttgcggaa atgctgcgcc aggtgatggc
     8401 cacaatgcaa cagcagcagc agcaacataa gccaccgcag ccaccgatgg ctaacaatcc
     8461 gatgctcaat aatccgcacg tgcagcgacc acagtatccc atgatgtatc cacagggtca
     8521 acagcaacag cagcagcagc ccccacagca atctgtagat gcttcaccgc cgctggagaa
     8581 cttcaaccag cagatcgatc ttgttttcaa ttcgattatg aatggagcca atgatgttgc
     8641 gcagcccata ctgcgcgatg tcctgggcat ggtagctggc acgggcaatg ctcctggtcc
     8701 tccgcttagc aacggtttga acggagtcga tctccagcag cagcagcagc aacaacagca
     8761 gcagcagcaa agccgcctat tcgcaatgat gcagcaacaa caacagcagc agcaacaaca
     8821 acaggccaag ccaccgctag gacctggacc actttatccc aatcatctgg gcggacctgg
     8881 tcttcaccag gcgccgccca atggtggagg taatctgagc ggcgtcggaa acaccaactt
     8941 tatgggcaac gccggaaatg ctctgctcaa tgacaagccc tacaacaacg tgggcatgca
     9001 caacaacgtg ggcttgaaca tcggtggcgc aggagttgct ggtcacaata gtaataacaa
     9061 caatggaatg ctgaccaatg gcaacaactc ggtgcccttg tatcgccgcc aggcgcacac
     9121 cggaaacggg attggaggcg gaaatggtga ctacaacaac atgcatggaa tggatgctgg
     9181 cgccaacttg atagaggcac tcttccaggc aaacaactcg gtggtggatc actcggtgaa
     9241 gtcgtcggac catatgcacg gcctgctgta caacaatttc aatgggctga agggcggcgg
     9301 ccacgaactg gacctgttcc aggcgatggc gcccagtgtg ccgcactcgg aggcagctaa
     9361 tcatcaccag cagtcctctg gctcatcggc aaccacctat gcggctgtgc tgagccaggg
     9421 accacaggcg gtggttggcg gaggcgttgg aacaggacca tcgccgggac agtcgcaggc
     9481 gcaggcgggc gtggctggtt caggaacggg cggaaaagtc gcgccggcgg ttggtggtaa
     9541 cgatcttctg gagaaggatc cgtttgcggc catccgggag ttgggacagg ccacgaccgg
     9601 attctacaac tattttcagt aatctagagg gcccgcggtt cgaaggtaag cctatcccta
     9661 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
     9721 aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga
     9781 tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     9841 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     9901 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     9961 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
    10021 atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
    10081 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
    10141 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
    10201 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
    10261 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
    10321 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
    10381 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
    10441 caggtagatg acgaccatca gggacagctt caaggccagc aaaaggccag gaaccgtaaa
    10501 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10561 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10621 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10681 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10741 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10801 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10861 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10921 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    10981 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11041 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11101 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11161 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11221 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11281 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11341 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11401 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11461 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11521 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11581 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11641 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11701 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11761 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11821 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11881 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11941 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    12001 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12061 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12121 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12181 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12241 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    12301 ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
