LOCUS       sequence-358324-        1847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1847)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046950
FEATURES             Location/Qualifiers
     source          1..1847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..35
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    82..106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             121..143
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             265..285
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             286..303
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     rep_origin      424..1012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     913..932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1118..1777
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1184..1203)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttcagaacgc tcggttgccg ccgggcgttt tttattggtc atcgatctcg atcccgcgaa
       61 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
      121 tttgtttaac tttaagaagg agactcttct atgggttaca tcaatgtatt tgcattccca
      181 ttcacgattt atagcctgtt gctgtgccgc atgaactctc gtaactacat cgcccaagtc
      241 gacgtggtta actttaacct tacagagaac ctgtactttc aaagccacca tcatcaccat
      301 cattaaccgg cttatcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
      361 tttgcttttg gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaaa
      421 agcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      481 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      541 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      601 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      661 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      721 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
      781 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
      841 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      901 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      961 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aattgatcgg
     1021 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     1081 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1141 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     1201 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     1261 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     1321 tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     1381 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     1441 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     1501 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tttcagccaa
     1561 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1621 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1681 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1741 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt gatatcgagc tcgcttggac
     1801 tcctgttgat agatccagta atgacctcag aactccatct ggatttg
//
