LOCUS       sequence-358325-        5903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway ORF clone of human PIK3CB [NM_006219.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5903)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E166 Hs.PIK3CB-nostop
FEATURES             Location/Qualifiers
     source          1..5903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..45
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     37..54
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     38..54
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             394..402
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1701..1712)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3394..3493)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3870..4661
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4754..5342
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5243..5262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5496..5513
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5672..5699)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5791..5877)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaaa
       61 attcgagctc ggtccgagga tccgaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagttg
      181 gcaccatgtg cttcagtttc ataatgcctc ctgctatggc agacatcctt gacatctggg
      241 cggtggattc acagatagca tctgatggct ccatacctgt ggatttcctt ttgcccactg
      301 ggatttatat ccagttggag gtacctcggg aagctaccat ttcttatatt aagcagatgt
      361 tatggaagca agttcacaat tacccaatgt tcaacctcct tatggatatt gactcctata
      421 tgtttgcatg tgtgaatcag actgctgtat atgaggagct tgaagatgaa acacgaagac
      481 tctgtgatgt cagacctttt cttccagttc tcaaattagt gacaagaagt tgtgacccag
      541 gggaaaaatt agactcaaaa attggagtcc ttataggaaa aggtctgcat gaatttgatt
      601 ccttgaagga tcctgaagta aatgaatttc gaagaaaaat gcgcaaattc agcgaggaaa
      661 aaatcctgtc acttgtggga ttgtcttgga tggactggct aaaacaaaca tatccaccag
      721 agcatgaacc atccatccct gaaaacttag aagataaact ttatggggga aagctcatcg
      781 tagctgttca ttttgaaaac tgccaggacg tgtttagctt tcaagtgtct cctaatatga
      841 atcctatcaa agtaaatgaa ttggcaatcc aaaaacgttt gactattcat gggaaggaag
      901 atgaagttag cccctatgat tatgtgttgc aagtcagcgg gagagtagaa tatgtttttg
      961 gtgatcatcc actaattcag ttccagtata tccggaactg tgtgatgaac agagccctgc
     1021 cccattttat acttgtggaa tgctgcaaga tcaagaaaat gtatgaacaa gaaatgattg
     1081 ccatagaggc tgccataaat cgaaattcat ctaatcttcc tcttccatta ccaccaaaga
     1141 aaacacgaat tatttctcat gtttgggaaa ataacaaccc tttccaaatt gtcttggtta
     1201 agggaaataa acttaacaca gaggaaactg taaaagttca tgtcagggct ggtctttttc
     1261 atggtactga gctcctgtgt aaaaccatcg taagctcaga ggtatcaggg aaaaatgatc
     1321 atatttggaa tgaaccactg gaatttgata ttaatatttg tgacttacca agaatggctc
     1381 gattatgttt tgctgtttat gcagttttgg ataaagtaaa aacgaagaaa tcaacgaaaa
     1441 ctattaatcc ctctaaatat cagaccatca ggaaagctgg aaaagtgcat tatcctgtag
     1501 cgtgggtaaa tacgatggtt tttgacttta aaggacaatt gagaactgga gacataatat
     1561 tacacagctg gtcttcattt cctgatgaac tcgaagaaat gttgaatcca atgggaactg
     1621 ttcaaacaaa tccatatact gaaaatgcaa cagctttgca tgttaaattt ccagagaata
     1681 aaaaacaacc ttattattac cctcccttcg ataagattat tgaaaaggca gctgagattg
     1741 caagcagtga tagtgctaat gtgtcaagtc gaggtggaaa aaagtttctt cctgtattga
     1801 aagaaatctt ggacagggat cccttgtctc aactgtgtga aaatgaaatg gatcttattt
     1861 ggactttgcg acaagactgc cgagagattt tcccacaatc actgccaaaa ttactgctgt
     1921 caatcaagtg gaataaactt gaggatgttg ctcagcttca ggcgctgctt cagatttggc
     1981 ctaaactgcc cccccgggag gccctagagc ttctggattt caactatcca gaccagtacg
     2041 ttcgagaata tgctgtaggc tgcctgcgac agatgagtga tgaagaactt tctcaatatc
     2101 ttttacaact ggtgcaagtg ttaaaatatg agccttttct tgattgtgcc ctctctagat
     2161 tcctattaga aagagcactt ggtaatcgga ggatagggca gtttctattt tggcatctta
     2221 ggtcagaagt gcacattcct gctgtctcag tacaatttgg tgtcatcctt gaagcatact
     2281 gccggggaag tgtggggcac atgaaagtgc tttctaagca ggttgaagca ctcaataagt
     2341 taaaaacttt aaatagttta atcaaactga atgccgtgaa gttaaacaga gccaaaggga
     2401 aggaggccat gcatacctgt ttaaaacaga gtgcttaccg ggaagccctc tctgacctgc
     2461 agtcacccct gaacccatgt gttatcctct cagaactcta tgttgaaaag tgcaaataca
     2521 tggattccaa aatgaagcct ttgtggctgg tatacaataa caaggtattt ggtgaggatt
     2581 cagttggagt gatttttaaa aatggtgatg atttacgaca ggatatgttg acactccaaa
     2641 tgttgcgctt gatggattta ctctggaaag aagctggttt ggatcttcgg atgttgcctt
     2701 atggctgttt agcaacagga gatcgctctg gcctcattga agttgtgagc acctctgaaa
     2761 caattgctga cattcagctg aacagtagca atgtggctgc tgcagcagcc ttcaacaaag
     2821 atgcccttct gaactggctt aaagaataca actctgggga tgacctggac cgagccattg
     2881 aggaatttac actgtcctgt gctggctact gtgtagcttc ttatgtcctt gggattggtg
     2941 acagacatag tgacaacatc atggtcaaaa aaactggcca gctcttccac attgactttg
     3001 gacatattct tggaaatttc aaatctaagt ttggcattaa aagggagcga gtgcctttta
     3061 ttcttaccta tgatttcatc catgtcattc aacaaggaaa aacaggaaat acagaaaagt
     3121 ttggccggtt ccgccagtgt tgtgaggatg catatctgat tttacgacgg catgggaatc
     3181 tcttcatcac tctctttgcg ctgatgttga ctgcagggct tcctgaactc acatcagtca
     3241 aagatataca gtatcttaag gactctcttg cattagggaa gagtgaagaa gaagcactca
     3301 aacagtttaa gcaaaaattt gatgaggcgc tcagggaaag ctggactact aaagtgaact
     3361 ggatggccca cacagttcgg aaagactaca gatcttcaac tttcttgtac aaagttggca
     3421 ttataggaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     3481 aaatcattat ttgcatgcaa gcttagatcc tctagaccag ccaggacaga aatgcctcga
     3541 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3601 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3661 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3721 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3781 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3841 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3901 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3961 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     4021 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     4081 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     4141 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     4201 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     4261 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     4321 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     4381 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4441 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4501 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4561 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4621 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4681 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4741 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4801 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4861 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4921 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4981 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5041 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5101 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5161 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5221 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5281 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5341 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5401 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5461 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5521 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5581 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     5641 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     5701 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     5761 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     5821 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     5881 cctggcagtt ccctactctc gcg
//
