LOCUS       sequence-358336-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of huamn TTR mutants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Ferguson JA, Sun X, Dyson HJ, Wright PE
  TITLE     Thermodynamic Stability and Aggregation Kinetics of EF Helix and EF 
            Loop Variants of Transthyretin.
  JOURNAL   Biochemistry. 2021 Mar 16;60(10):756-764. doi: 
            10.1021/acs.biochem.1c00073. Epub 2021 Mar 1.
  PUBMED    33645214
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.1c00073"; journalName: "Biochemistry"; date: 
            "2021-03-16- 16"; volume: "60"; issue: "10"; pages: "756-764"
COMMENT     Sequence Label: TTR C10S S85C K80E
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..100
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        81..99
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    100..124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             139..161
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(604..622)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
       61 cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      121 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat gggtcctacg
      181 ggcaccggtg aatccaagag tcctctgatg gtcaaagttc tagatgctgt ccgaggcagt
      241 cctgccatca atgtggccgt gcatgtgttc agaaaggctg ctgatgacac ctgggagcca
      301 tttgcctctg ggaaaaccag tgagtctgga gagctgcatg ggctcacaac tgaggaggaa
      361 tttgtagaag ggatatacaa agtggaaata gacaccaaat cttactggga ggcacttggc
      421 atctgcccat tccatgagca tgcagaggtg gtattcacag ccaacgactc cggcccccgc
      481 cgctacacca ttgccgccct gctgagcccc tactcctatt ccaccacggc tgtcgtcacc
      541 aatcccaagg aatgagatcc ggctgctaca aagcccgaaa ggaagctgag ttggctgctg
      601 ccaccgctga gcaataacta gcataacccc
//
