LOCUS       sequence-358344-       10363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6A-delMI-MII-AGOhook.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A-delMI-MII-AGOhook_F
FEATURES             Location/Qualifiers
     source          1..10363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5105..5116
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    6067..6188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6078..6097)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6132..6151
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6374..6829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6461..6480)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6671..6692
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6946..7303
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6946..7137
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6969..6989)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7154..7289
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7354..8148
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7408..7427)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8018..8037
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8212..8260
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8301..8320)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8420..8442
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8480..8498)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8566..8670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8671..8739,8740..9531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8889..8908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9702..10290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10191..10210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggca
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acagtggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattaat gggacagagg
     2581 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2641 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2701 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2761 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2821 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     2881 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     2941 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3001 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3061 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3121 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3181 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3241 ggggacctat accagcccca gcaaaagaag aagtcccaaa tggcaacagc cgttcagacc
     3301 agcaagcaca ggtacatcag ctgctaacgc ctgcaagtgc catctcaaac aaagaggcaa
     3361 gcagtggctc tggctggggt gagccctggg gggagccttc tactccagcc acaactgctg
     3421 cggcatccag cacatccacg tggggctcca gctctgttgg tccacaagca ttaagcaaat
     3481 ctgggccaaa atctatgcaa gatggctggt gtggtgatga tatgccattg cctggaaatc
     3541 gccccactgg ctgggaagag gaagaggatg tggagattgg aatgtggaat agtaattcat
     3601 ctcaagagct taactcatct ttaaattggc caccatatac aaagaaaatg tcatcgaagg
     3661 gtctgagtgg caaaaaaagg agaagggaag ggggaatgat gaaaggtgga aacaaacaag
     3721 aagaagcgtg gataaatcca tttgttaaac agttttcaaa catcagtttt tcgagagact
     3781 caccagagga aaatgtacaa agcaataaga tggacctttc tggaggaatg ttacaagaca
     3841 aacgaatgga gatagataaa catagcctaa atattggtga ttacaatcga acggtcggga
     3901 aaggccctgg ttctcggcct cagatttcca aagagtcttc catggagcgc aatccttatt
     3961 ttgataagga tggcattgta gcagatgaat cccaaaacat gcagtttatg tccagtcaaa
     4021 gcatgaagct tcccccttca aatagtgcac tacctaacca ggcccttggc tccatagcag
     4081 ggctgggtat gcaaaacttg aattctgtta gacagaatgg caatcccagt atgtttggtg
     4141 ttggaaacac agcagcacaa ccccggggca tgcagcagcc tccagcacaa cctcttagtt
     4201 catctcagcc taatctccgt gctcaagtgc ctcctccatt actctcccct caggttccag
     4261 tttcattgct gaagtatgca ccaaacaacg gtggcctgaa tccactcttt ggccctcaac
     4321 aggtagccat gctgaaccag ctatcccagc taaaccagct ttctcagatc tcccagttac
     4381 agcgattgtt agcgcagcag caaagggcgc agagtcagag aagcgtgcct tctgggaacc
     4441 ggccgcagca agaccagcag ggtcgacctc ttagtgtgca gcagcaaatg atgcaacaat
     4501 ctcgtcaact tgatccaaac ctgttggtga agcagcagac tccaccatct cagcagcagc
     4561 cactccatca gccagccatg aagtctttcc ttgacaatgt catgccccac actacacctg
     4621 agctgcaaaa agggccatca ccaataaatg ctttcagcaa cttccctata ggcttgaact
     4681 caaacttgaa tgtaaatatg gatatgaaca gtattaaaga gccacagtca agactaagga
     4741 agtggacgac agtgggcagc atttctgtga acacatcttt ggatcaaaac tccagcaaac
     4801 atggtgctat ttcaagtggt ttcaggctgg aagagtctcc atttgttccc tatgacttta
     4861 tgaacagcag tacttcacca gccagtcctc caggttcaat aggagatggc tggccacgtg
     4921 ccaaatcgcc taacggctct agcagtgtta attggccacc agaatttcgt cctggtgagc
     4981 catggaaagg ttatccaaac attgaccctg aaactgaccc ttacgtcact cctggcagtg
     5041 tcataaacaa tctttcaatt aatactgtgc gggaagttga ccacctcagg gacaggaaca
     5101 gtgggtcatc ctcatccttg aacaccacgc tgccttcaac tagtgcctgg tcatccattc
     5161 gtgcctccaa ctacaacgtt cccctcagca gtacagcaca aagcacttca gccagaaata
     5221 gtgattccaa attgacatgg tctcctggtt cagttacaaa cacctctctg gctcatgagc
     5281 tgtggaaggt ccctttgcca cctaaaaaca tcactgctcc gtcccgccca cctccgggac
     5341 tgactggtca gaagccaccc ttgtctacgt gggataattc tccccttcgt ataggtggag
     5401 gatggggaaa ttctgacgcc agatataccc caggttccag ctggggtgag agcagctcag
     5461 ggagaataac aaattggctt gttctaaaaa accttacacc tcagatcgat ggctcaactc
     5521 tgcgcactct gtgcatgcag cacggcccgc tgatcacatt ccacctgaac ctccctcacg
     5581 gaaatgctct ggtccgctac agttcaaaag aagaggtagt ggaggcacaa aagtctctgc
     5641 acatgtgtgt actggggaac actactattc ttgctgagtt tgccagtgaa gaggagatca
     5701 gtcgtttctt tgcacaaagc cagtctctga ccccttctcc cggctggcag tctctcgggt
     5761 ccagccagag ccggctgggc tccctcgact gttcccactc attctccagc cggaccgatc
     5821 tcaatcactg gaatggtgct gggctgtcgg gaactaactg tggagacctt cacggcactt
     5881 cactctgggg gaccccgcat tattccacaa gcctgtgggg tcccccaagc agcagcgacc
     5941 cccgaggaat tagcagccca tctcccatta acgcttttct ttctgttgac cacctgggtg
     6001 ggggtggaga gtccatgtaa gcggccgctt ccctttagtg agggttaatg cttcgagcag
     6061 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     6121 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6181 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     6241 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatccgataa ggatcgatcc
     6301 gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6361 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6421 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6481 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6541 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6601 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6661 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6721 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6781 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
     6841 attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct gcgcagcacc
     6901 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     6961 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7021 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7081 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7141 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7201 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7261 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga
     7321 cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg attgcacgca
     7381 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7441 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7501 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7561 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7621 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7681 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7741 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7801 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7861 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7921 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7981 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8041 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8101 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     8161 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
     8221 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
     8281 cgcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     8341 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8401 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8461 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8521 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8641 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8701 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8761 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8821 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8881 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8941 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9001 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9061 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9121 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9181 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9241 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9301 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9361 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9421 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9481 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9541 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9841 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10321 ctggcctttt gctggccttt tgctcacatg gctcgacaga tct
//
