LOCUS       sequence-358347-        4410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC27A6_STOP
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1967..2066)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2083..2102)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2084..2102)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2107..2123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2107..2123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2225..2244)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2236..3045
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2303..2322)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3192..3780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3681..3700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3934..3951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4110..4137)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4229..4315)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4364..4386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4378..4395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4379..4395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgct gctgtcctgg
      121 ctgaccgtgc tgggagcagg catggtggtg ctgcacttcc tgcagaagct gctgttcccc
      181 tatttttggg acgatttctg gtttgtgctg aaggtggtgc tgatcatcat ccgcctgaag
      241 aagtacgaga agcggggcga gctggtgaca gtgctggata agtttctgtc tcacgccaag
      301 cggcagccaa gaaagccctt catcatctat gagggcgaca tctacaccta tcaggacgtg
      361 gataagagga gctcccgcgt ggcccacgtg tttctgaacc actctagcct gaagaagggc
      421 gacacagtgg ccctgctgat gagcaatgaa cccgattttg tacacgtctg gtttggactg
      481 gcaaagctgg gatgcgtggt ggccttcctg aacaccaata tcagatctaa cagcctgctg
      541 aattgcatca gggcctgtgg accacgcgcc ctggtggtgg gagcagatct gctgggcaca
      601 gtggaggaga tcctgccctc cctgtctgag aacatcagcg tgtggggcat gaaggattcc
      661 gtgcctcagg gcgtgatctc tctgaaggag aagctgagca cctccccaga cgagccagtg
      721 cctagaagcc accacgtggt gtccctgctg aagtctacct gcctgtacat ctttacatcc
      781 ggaaccacag gactgcctaa ggcagccgtg atcagccagc tgcaggtgct gaggggatcc
      841 gccgtgctgt gggccttcgg ctgtaccgcc cacgacatcg tgtacatcac actgccactg
      901 tatcactcct ctgccgcaat cctgggaatc tccggatgcg tggagctggg agcaacatgc
      961 gtgctgaaga agaagttcag cgccagccag ttttggtctg actgcaagaa gtacgatgtg
     1021 accgtgtttc agtatatcgg cgagctgtgc agatacctgt gcaagcagtc taagagggag
     1081 ggcgagaagg atcacaaggt gaggctggca atcggaaacg gaatccggag cgacgtgtgg
     1141 agagagttcc tggataggtt tggcaatatc aaggtgtgcg agctgtatgc cgccacagag
     1201 agctccatca gcttcatgaa ctacaccgga aggatcggag caatcggaag gacaaatctg
     1261 ttctataagc tgctgtccac ctttgacctg atcaagtacg acttccagaa ggatgagccc
     1321 atgcgcaacg agcagggctg gtgcatccac gtgaagaagg gagagcctgg actgctgatc
     1381 tccagggtga acgccaagaa tccattcttt ggctacgccg gcccctataa gcacacaaag
     1441 gacaagctgc tgtgcgacgt gttcaagaag ggcgacgtgt atctgaacac cggcgatctg
     1501 atcgtgcagg accaggataa tttcctgtac ttttgggaca gaaccggcga taccttccgg
     1561 tggaagggcg agaacgtggc caccacagag gtggccgacg tgatcggcat gctggatttc
     1621 atccaggagg ccaacgtgta cggcgtggca atcagcggat acgagggaag ggcaggcatg
     1681 gcctctatca tcctgaagcc caatacaagc ctggacctgg agaaggtgta tgagcaggtg
     1741 gtgacctttc tgcctgccta cgcctgtcca cggttcctga gaatccagga gaagatggag
     1801 gccaccggca cattcaagct gctgaagcac cagctggtgg aggacggctt taaccccctg
     1861 aagatctccg agcctctgta cttcatggat aatctgaaga agtcttatgt gctgctgacc
     1921 cgggagctgt acgaccagat catgctgggc gagatcaagc tgtaagaccc agctttcttg
     1981 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     2041 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     2101 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2161 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2221 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2281 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2341 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2401 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2461 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2521 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2581 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2641 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2701 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2761 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2821 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2881 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2941 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3001 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3061 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3121 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3301 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3541 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3661 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3961 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4021 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4081 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4141 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4201 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4261 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4321 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4381 aaaacgacgg ccagtcttaa gctcgggccc
//
