LOCUS       sequence-358371-       10602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral eukaryotic expression vector with E.Coli Dam methylation 
            fused to mouse Lamin A. Used in DamID experiments to methylate DNA 
            that interacts in the proximity of Lamin A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Meuleman W, Peric-Hupkes D, Kind J, Beaudry JB, Pagie L, Kellis M, 
            Reinders M, Wessels L, van Steensel B
  TITLE     Constitutive nuclear lamina-genome interactions are highly conserved
            and associated with A/T-rich sequence.
  JOURNAL   Genome Res. 2013 Feb;23(2):270-80. doi: 10.1101/gr.141028.112. Epub 
            2012 Nov 2.
  PUBMED    23124521
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.141028.112"; journalName: "Genome Res"; date: "2013-02";
            volume: "23"; issue: "2"; pages: "270-80"
COMMENT     Sequence Label: pLenti GW V5 Eco/Dam mse LaminA
FEATURES             Location/Qualifiers
     source          1..10602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(116..135)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        307..686
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        688..886
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     841..861
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             904..1084
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1131..1256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1749..1982
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2167..2211
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2673..2911
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2858..2879
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3806..3847
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3863..3887
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5921..5945)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             6476..6656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6682..6699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6688..6912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6958..7386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7045..7064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7255..7276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7395..7415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7400..7729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7580..7715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7642..7661
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7777..7824
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7843..8217
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8347..8468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8384..8403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8438..8457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8517..8533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8517..8533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8530..8552)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8541..8557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8565..8571,8572..8589,8590..8595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8610..8631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8748..8765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8919..9507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8999..9018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9678..10469,10470..10538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10301..10320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10539..10602,1..41))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
       61 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
      121 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
      181 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
      241 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
      301 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
      361 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      421 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      481 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      541 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      601 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      661 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      721 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      781 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      841 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      901 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      961 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     1021 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1081 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     1141 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     1201 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     1261 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1321 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1381 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1441 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1501 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1561 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1621 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1681 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1741 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1801 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1861 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1921 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1981 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2041 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2101 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2161 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2221 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2281 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2341 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2401 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcc
     2461 cccgggctgc aggaattcag atctcggccg catattaagt gcattgttct cgataccgct
     2521 aagtgcattg ttctcgttag ctcgatggac aagtgcattg ttctcttgct gaaagctcga
     2581 tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg ttctcttgct
     2641 gaaagctcag tacccgggag taccctcgac cgccggagta taaatagagg cgcttcgtct
     2701 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     2761 aaataaacaa gcgcagctga acaagctaaa caatctgcag taaagtgcaa gttaaagtga
     2821 atcaattaaa agtaaccagc aaccaagtaa atcaactgca actactgaaa tctgccaaga
     2881 agtaattatt gaatacaaga agagaactct gaatactttc aacaagttac cgagaaagaa
     2941 gaactcacac acagctagcg ccaccatgaa gaaaaatcgc gcttttttga agtgggcagg
     3001 gggcaagtat cccctgcttg atgatattaa acggcatttg cccaagggcg aatgtctggt
     3061 tgagcctttt gtaggtgccg ggtcggtgtt tctcaacacc gacttttctc gttatatcct
     3121 tgccgatatc aatagcgacc tgatcagtct ctataacatt gtgaagatgc gtactgatga
     3181 gtacgtacag gccgcacgcg agctgtttgt tcccgaaaca aattgcgccg aggtttacta
     3241 tcagttccgc gaagagttca acaaaagcca ggatccgttc cgtcgggcgg tactgttttt
     3301 atatttgaac cgctacggtt acaacggcct gtgtcgttac aatctgcgcg gtgagtttaa
     3361 cgtgccgttc ggccgctaca aaaaacccta tttcccggaa gcagagttgt atcacttcgc
     3421 tgaaaaagcg cagaatgcct ttttctattg tgagtcttac gccgatagca tggcgcgcgc
     3481 agatgatgca tccgtcgtct attgcgatcc gccttatgca ccgctgtctg cgaccgccaa
     3541 ctttacggcg tatcacacaa acagttttac gcttgaacaa caagcgcatc tggcggagat
     3601 cgccgaaggt ctggttgagc gccatattcc agtgctgatc tccaatcacg atacgatgtt
     3661 aacgcgtgag tggtatcagc gcgcaaaatt gcatgtcgtc aaagttcgac gcagtataag
     3721 cagcaacggc ggcacacgta aaaaggtgga cgaactgctg gctttgtaca aaccaggagt
     3781 cgtttcaccc gcgaaaaaaa ccggtggtaa gcctatccct aaccctctcc tcggtctcga
     3841 ttctacgatc gatttcgatc aaacaagttt gtacaaaaaa gcaggctccg cggccgcccc
     3901 cttcaccatg gagaccccgt cacagcggcg cgccacccgc agtggggcgc aggccagctc
     3961 taccccactg tcgcccactc ggatcacccg gctgcaggag aaggaggacc tgcaggagct
     4021 caatgaccgc ctggccgtgt acatcgatcg cgtgcgttcc ctggagaccg agaacgcggg
     4081 gctgcgcctt cgcatcactg agtctgaaga ggtggtcagc cgagaggtgt ccggcatcaa
     4141 ggcggcctac gaggccgagc tgggggatgc ccgcaagacc cttgattctg tggccaagga
     4201 gcgcgcccgc ctccagctag agctgagcaa agtgcgtgag gagttcaagg agctgaaggc
     4261 tcgcaacacc aagaaggagg gggacttgtt ggctgcgcag gcccggctca aggacctcga
     4321 ggctcttctc aactccaagg aagctgccct gagcactgct ctcagtgaga agcgcacatt
     4381 ggagggcgag ctccatgacc tgcgggggca ggtagccaag cttgaggcgg ccctgggaga
     4441 ggctaagaag cagcttcagg atgagatgct gaggcgagtg gatgctgaga acaggctaca
     4501 gacgctgaag gaggagcttg acttccagaa gaacatttac agcgaggaac tgcgtgagac
     4561 caagcgccgg catgagacgc ggcttgtgga gatcgataac gggaagcagc gagagtttga
     4621 gagccggctg gcagatgccc tgcaggagct gcgggctcag catgaggacc aggtggaaca
     4681 gtataagaag gagctagaaa agacatactc cgccaagctg gataatgcca ggcagtctgc
     4741 tgagaggaac agcaacctcg tgggggctgc ccatgaggaa ctgcagcagt ctcgaatccg
     4801 cattgacagc ctctcggccc agctcagcca gctccaaaag cagttggcag ccaaggaggc
     4861 aaagctgcgt gacctggagg actcgctggc ccgtgagcgc gataccagcc ggcgcctgct
     4921 ggctgagaaa gagcgagaga tggcggagat gcgggcgagg atgcagcagc agctggacga
     4981 gtaccaggag ctgctggaca tcaagctggc cctggacatg gagatccatg cctaccgaaa
     5041 gctgctggag ggcgaggagg agaggctgcg cctgtccccc agccctacct cgcagcgcag
     5101 ccgtggccgc gcctcctccc actcatccca gtctcagggt ggaggcagcg tcaccaaaaa
     5161 gcgcaagctg gagtcttccg agagccggag cagcttctcg cagcatgctc gcactagcgg
     5221 gcgtgtggcg gtagaggaag tcgatgaaga gggaaagttc gtgcggctgc gcaacaagtc
     5281 caacgaggac cagtccatgg gcaactggca gatcaggcgt cagaatggtg acgatccttt
     5341 gatgacctat cgcttcccac cgaagttcac cctaaaggct gggcaggtgg tgacgatctg
     5401 ggcttcagga gctggggcca cccatagccc ccctactgac ttggtgtgga aggcgcagaa
     5461 cacctggggc tgtgggagca gccttcgcac cgctctcatc aactccactg gagaagaagt
     5521 ggccatgcgc aagctggtgc gctcactgac catggttgag gacaatgagg atgacgacga
     5581 ggatggagaa gagctcctcc atcaccaccg tggttcccac tgcagcggct cgggggaccc
     5641 cgctgagtac aacctgcgct cacgcaccgt gctgtgcggg acgtgtgggc agcctgctga
     5701 caaggctgcc ggtggagcgg gagcccaggt gggcggatcc atctcctctg gctcttctgc
     5761 ctccagtgtc acagtcactc gaagcttccg cagtgtgggg ggcagtgggg gtggcagctt
     5821 cggggacaac ctagtcaccc gctcctacct cctgggcaac tccagtcccc ggagccagag
     5881 ctcccagaac tgcagcatca tgtaaagggt gggcgcgccg acccagcttt cttgtacaaa
     5941 gtggttcgat cgaaccgcgg tctagactcg agacctagaa aaacatggag caatcacaag
     6001 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6061 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6121 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6181 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6241 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6301 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6361 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6421 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     6661 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6721 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6781 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6841 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6901 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6961 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7021 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7081 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7141 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7201 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7261 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7321 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7381 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7501 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7741 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     7801 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     7861 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     7921 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     7981 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8041 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8101 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8161 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8221 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8281 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8341 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8401 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8461 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8521 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8581 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8641 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8701 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8761 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8821 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8881 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8941 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9001 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9061 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9121 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9181 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9241 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9301 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9361 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9421 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9481 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9541 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9601 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9661 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9721 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9781 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9841 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9901 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9961 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10021 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10081 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10141 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10201 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10261 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10321 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10381 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10441 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10501 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10561 tgaagcattt atcagggtta ttgtctcatg agcggataca ta
//
