LOCUS       sequence-35839-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Landthaler M, Yalcin A, Tuschl T
  TITLE     The human DiGeorge syndrome critical region gene 8 and Its D. 
            melanogaster homolog are required for miRNA biogenesis.
  JOURNAL   Curr Biol. 2004 Dec 14. 14(23):2162-7.
  PUBMED    15589161
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2004 Dec 14. 14(23):2162-7."
COMMENT     Sequence Label: pcDNA4/TO/cmycDrosha
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     50..74
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             168..197
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag
       61 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      121 gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttatggaa caaaaactta
      181 tttctgaaga agatctgatg atgcagggaa acacatgtca cagaatgtcg ttccacccgg
      241 gacgagggtg tccccgagga cgaggaggac atggagccag accctcagca ccatccttta
      301 ggccccaaaa tctgaggctg cttcaccctc agcagcctcc tgtgcaatat caatatgaac
      361 ctccaagtgc cccttccacc actttctcaa actctccagc ccccaatttt ctccctccac
      421 gaccagactt tgtacccttc cccccaccca tgcctccgtc agcgcaaggc cctcttcccc
      481 cctgcccaat caggccgcct ttccccaacc accagatgag gcaccccttc ccagttcctc
      541 cttgttttcc tcccatgcca ccaccaatgc cttgtcctaa taacccccca gtccctgggg
      601 cacctcctgg acaaggcact ttccccttca tgatgccccc tccctccat
//
