LOCUS       sequence-358429-        8307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAP259 derived plasmid encoding a xylose inducible BitlucOpt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Phothichaisri W, Chankhamhaengdecha S, Janvilisri T, Nuadthaisong J,
            Phetruen T, Fagan RP, Chanarat S
  TITLE     Potential Role of the Host-Derived Cell-Wall Binding Domain of 
            Endolysin CD16/50L as a Molecular Anchor in Preservation of 
            Uninfected Clostridioides difficile for New Rounds of Phage 
            Infection.
  JOURNAL   Microbiol Spectr. 2022 Apr 4:e0236121. doi: 
            10.1128/spectrum.02361-21.
  PUBMED    35377223
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2022 Apr 4:e0236121. doi: 10.1128/spectrum.02361-21."
COMMENT     Sequence Label: pJAK175
FEATURES             Location/Qualifiers
     source          1..8307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5601..5972)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            6005..6114
                     /note="incP origin of transfer"
     primer_bind     complement(7305..7322)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7476..8064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7556..7575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgcaccaat tttttacttt tctattttag aatctagcat ttccaatgct tactaatact
       61 atttttgatt tttaacttgt tttaaaaaag cacctactaa ttaggattct agctctatat
      121 tctagttata cattctctat attctagctc tatattctag ttatacattc tctatattct
      181 agctctatat tctagttata cattctctat attctagctc tatattctag ttatacattc
      241 tctatattct agctctatat tctagttata cattctctat attctagctc tatattctag
      301 ttatacattc tctatattct agctctatat tctagttata cattctctaa aaaacatata
      361 tatatatact acatatatat gtacaattat tttaccaaaa gaaattttta aaacggctta
      421 atttcaatac tttgatgcca tttttttttt gcaattctcg tacaattatt ttaccaaaag
      481 aaatttttat taattagttt ttttgcataa aaataggact agaaaatcaa ttctaatcct
      541 atcaaaaatc tacttgttta atctatatgt atagtacata aatcctctat catttatttt
      601 agtttcccat acatctaaaa ttttagtatc taataaagat tctatcatat ttgaaattct
      661 ttgctgtgtt actcctaaat cttttgctaa ttcctcttga ttaattctta atatatttcc
      721 ttttatttta tcatctttga ccaataaatc ttgcttatat ctcatatgag tatataatct
      781 taattcttca gttgaaatta ttccccaaat tactgccatg gtaacaaagt aactaatagt
      841 atattgttta tctatattac atctaacttc ttttacattg tatgtatctt ttattcctag
      901 ctttgtaaca ccttttgttt ttgtaatata atcattatgt tgaagttctt ttaatgtttc
      961 tcttaatgtc ttttcactca taacaatgtt tttaactttc ttcttacgtt tataggttat
     1021 cagctccatt atatcgtcta tgtttaattc tctatcttta ttgttaagta acacattata
     1081 gataaacaat tgattaccag tcattatttt aacccctttc ctattcttat atttcaaatc
     1141 ctttgagtgc ttatgtggca tattgaacaa agtatcttca tcactatata taaaatctga
     1201 ttctatttta ttccaacaat cactattaaa ctcacaacct gtacaaccaa aattaaccgt
     1261 tttaagcttc tcatacatat acttgacttg atattctagc tcactatttg aaagactatt
     1321 ttgtgatata tagttccatt ctctagcaac ggatattact tgacttaaac ttttattcct
     1381 caatcttaaa gtcacaacta ttttttgcaa atctaggttt ttatgtccat ctttactacc
     1441 attttttaaa atatctacta tacaatttgg tagcttacta tctaatatat attttgtatt
     1501 agtattacct actgttttta catctttgca atatttttta gctaactttt ctatgtcata
     1561 tctatatata tttttgtctt ggtgtattat ttttacttgt ttagtagtat ttttaatatt
     1621 atatgtataa ggtactctta atacttgagt agataaattt gcttgtttat ctgcacccaa
     1681 tttatttatt aatgtatttg taacttctga tactaactta atgttattag ttttattaat
     1741 tagcacataa acatggaatc catttccact atcaactagg atatgataat gtaatcctat
     1801 cttagtaaat aaattaatta tatctttata attaaaacct tgtcctaatt cttttttgtc
     1861 aaaatcaaat cctaatgcat atctatttat taagttttca ttggttcctg cttttccatc
     1921 agttgtacat aggttaaaaa atgtattttc ataataaata ttttttgcga aggtaaattc
     1981 tactatatca tctatatttt taaaaaaagt tgttttacta tattctttaa attcttctcc
     2041 gagttctaat tgtttactat tacttttatt attttgaaat actctaacat attcattatc
     2101 aataagatta tttccttgtc ctataccatc tctaaatact gccattagaa attttttcaa
     2161 cttatatttt gaatctaatt gttccatata tttattatct ccttatctag tttttttaag
     2221 agataacact acattaaata tatacttatt gactaatcaa cttttacaac tattgaataa
     2281 ttttagctta gtatgtataa taatattgtt aagatgctaa aattgatagc aatattatta
     2341 tataactaca gatacttaat cagttagacc gatattggag ttggtagctc cataaactca
     2401 attggtagtt gagttgtcat tgtctagtag tattatctca ttttgttgtt atcttaaaat
     2461 attattttaa aaatactctg cttggtcggc ttagggtatt ttttttgttt aattccaata
     2521 ttggttcaaa ctcctgtgta atacttttga cacttccatc tgtattgaat tgacagttga
     2581 tatggtatct cattattggc ttgttcttat gtttttctat gtatttattt tttactttca
     2641 aataatttgt atttccattt ttagttatgt ttaacacact atccttatta ctcaataaaa
     2701 ttattttttg ttttaacata tgatttatag aatctaaaaa ttgatattga cttataatag
     2761 tactttctac ctctctagct ttttctagtg tttcaaattc ttcaaactct gctatattgc
     2821 tatagtatag cttatcatta ataatattat ctgacaactt taattccatt ttataaaaca
     2881 attccaagtt atgtataaaa tttttaatgt tacaacatat agacttatta tttgttctta
     2941 tatggtatct ccatttacta cttctagctc tcatgttata tgcacaacta ctcccaacat
     3001 aaacatatat tgcttgttct aactcatatt tttgtaaaat ttcaacatca tatccagtca
     3061 aatcgtactc tctcttatat acaaatggtt tttctaattt tttgttcaat aaatctcctc
     3121 ctatttctta cttaatttag ctaaaaatct atttatattt cacctccttt ttgactttaa
     3181 gcctacgaat accatattta tgattataac atcttctatt tttcccaaat ccttacatct
     3241 cccctaattt ttagacttaa gggcgaattc tgcatcaagc tagcataaaa ataagaagcc
     3301 tgcatttgca ggcttcttat ttttatggta ccggttatgg ctgtatatag tcataaccct
     3361 tatatttcat taattaaagt taaattttgt atattcaaaa agctcttgat atttacggca
     3421 gagccaccat atagagatgc ctcagaccct aatgatgaaa tttctatatt tattcctttg
     3481 ttaaaagagc taactaacat atcttttgta atctgtaata tctcagggat atcactaatt
     3541 atttggctat ttaaatatat tatttcagga gcataagttg taatagcatt atttatagct
     3601 attgttaagt aactacaaaa ctcatgaata acttttttgg cattctggtt atcttcataa
     3661 tatagttgct ttactatatc agaatctatt ttaggaatat tttctaaaga agaaagctgt
     3721 tcaaatacct ttttttctga acaatactgt tctaaacagc cacgatttcc acaaggacat
     3781 agtttaccat taggcatgat gatagtgtga ccaatttctc cactcatacc atttctgccg
     3841 ctatataatt tattatttat tataatgcct gaaccaaatc cactgtgaat actgagacta
     3901 agtagtgagt tatgtacagt tgaaaaagta ttctcagcta aagctgttaa gtttgcttca
     3961 ttttctatat gaataggaaa gtcatacttt ttgctaagga tgctatataa gtcaatctca
     4021 ttaagattat aatatggagt aaacaatact ttattttcac aagtaatccc atgtatagcc
     4081 aaagtaagac caattacctt ataaggagtg tctattttgg atatgttata actatttata
     4141 atttcatcta tcaattgtat aacattgtct ttacttactt gtatatctgt taatttctta
     4201 gaatttataa tagttccatc taagtaagat agagaagaaa atatatagtc gtatccaatg
     4261 tccatactta aagaaatccc tgcacattta ttaaatacta ataatatggg ttttcttccg
     4321 ccactatgag tactatttcc aattcctatt tcatgaacta gagattcatc aataagtttt
     4381 ttggtaatag cggatatagt tgctttattc aatcctatag tagaagctat acttgcccta
     4441 gaaataggac cattttttat aatttgttca agtaccaatc tttcattcat ttctcgaata
     4501 gtgtatttat cagtaaccaa tttgatattc ctccttaaaa taatattgta atacttttta
     4561 cacaaaaata aaaggttatt ttgcattgac aaagataatt aaatatttta ttattagttc
     4621 ataagttagt ttaatatact aacaaaaata aagcaagtaa aatataccta aaatataaaa
     4681 aaattaggat aggaaaacga tagttatgaa gtggcattcg agctcctgca gtaaaggaga
     4741 aaattttgtg tttacacttg aagattttgt gggtgattgg gaacaaactg ctgcatataa
     4801 tttagatcaa gttttagaac agggtggagt gagttctctt ttacaaaatc ttgcagtctc
     4861 agttacacca atacaaagaa tagttagaag tggagaaaat gcattaaaga tcgatataca
     4921 tgtaataatt ccttatgagg gactatcagc agaccaaatg gcacaaattg aggaagtatt
     4981 caaagttgta tatccagtag acgatcatca ctttaaagta atattacctt atggaacttt
     5041 agtaattgat ggtgtaacac caaatatgtt aaattatttt ggtagaccat acgaagggat
     5101 tgcagttttt gatggaaaga aaataaccgt aacaggcact ttgtggaatg gaaataaaat
     5161 aatagatgaa aggttaatta cacctgatgg atctatgttg tttagagtaa ctataaacag
     5221 tgttacaggt tatagacttt ttgaagaaat tctataggga tcctataagt tttaataaaa
     5281 ctttaaatag aaaaaggctt ctctcatgag aagtcttttt tatttaaaat aaatataaaa
     5341 taaaatagag gctataaata gcctctattt tatgtgagaa atccctaaat aaaaagatgc
     5401 cagtgtgctg gaattcgccc ttaggctgcg ctagggacct ctttagctcc ttggaagctg
     5461 tcagtagtag gggatcggtc ttgccttgct cgtcggtgat gtacttcacc agctccgcga
     5521 agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc
     5581 cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc
     5641 ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca
     5701 ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg
     5761 cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg
     5821 attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc
     5881 gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg
     5941 cccttcctgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg
     6001 gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     6061 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     6121 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     6181 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca ggaagggcga
     6241 attctgcaga tctgaccggt ctctgaaaat ataaaaacca cagattgata ctaaaacctt
     6301 ggttgtgttg cttttcgggg cttaaatcaa ggaaaaatcc ttgttttaag cctttcaaaa
     6361 agaaacacaa ggtctttgta ctaacctgtg gttatgtata aaattgtaga ttttagggta
     6421 acaaaaaaca ccgtatttct acgatgtttt tgcttaaata cttgttttta gttacagaca
     6481 aacctgaagt taactattta tcaattcctg caattcgttt acaaaacggc aaatgtgaaa
     6541 tccgtcacat actgcgtgat gaacttgaat tgccaaagga agtataattt tgttatcttc
     6601 tttataatat ttccccatag taaaaatagg aatcaaataa tcatatcctt tctgcaaatt
     6661 cagattaaag ccatcgaagg ttgaccacgg tatcatagat acattaaaaa tgttttccgg
     6721 agcatttggc tttccttcca ttctatgatt gtttccatac cgttgcgtat cactttcata
     6781 atctgctaaa aatgatttaa agtcagactt acactcagtc caaaggctgg aaaatgtttc
     6841 agtatcattg tgaaatattg tatagcttgg tatcatctca tcatatatcc ccaattcacc
     6901 atcttgattg attgccgtcc taaactctga atggcggttt acaatcattg caatataata
     6961 aagcattgca ggatatagtt tcattccctt ttcctttatt tgtgtgatat ccactttaac
     7021 ggtcatgctg taggtacaag gtacacttgc aaagtagtgg tcaaaatact cttttctgtt
     7081 ccaactattt ttatcaattt tttcaaatac catctaagtt ccctctcaaa ttcaagttta
     7141 tcgctctaat gaacaaagat attataccac atttttgtga atttttcaac ttgcccactt
     7201 cgactgcact cccgacttaa taacttcttg aacacttgcc gaaaaagaaa aactgccggg
     7261 tacgtacccg gggatcgatc cccggccgag cgctcttccg cttcctcgct cactgactcg
     7321 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7381 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7441 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7501 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7561 taccaggcgt ttcccccttg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7621 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7681 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7741 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7801 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7861 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7921 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8041 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8101 cagtggaacg aaaactcacg ttaagggatt ttggtcatga aatgcaagtt tctaactaac
     8161 atacatcata gttactaaac tatggtaact ataattttat taactatatt aagcactgat
     8221 tagtactata actcaatata agcatatccc ctgtatcgta actagagaac caaacgacgg
     8281 aaaaagcgat atagataagt ttagata
//
