LOCUS       sequence-35843-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_OCT4
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     137..156
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        137..155
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      195..204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccggactct agaggatcga acccttttgg
      121 accctcgtac agaagctaat acgactcact atagggaaat aagagagaaa agaagagtaa
      181 gaagaaatat aagagccacc atggcgggac acctggcttc ggatttcgcc ttctcgcccc
      241 ctccaggtgg tggaggtgat gggccagggg ggccggagcc gggctgggtt gatcctcgga
      301 cctggctaag cttccaaggc cctcctggag ggccaggaat cgggccgggg gttgggccag
      361 gctctgaggt gtgggggatt cccccatgcc ccccgccgta tgagttctgt ggggggatgg
      421 cgtactgtgg gccccaggtt ggagtggggc tagtgcccca aggcggcttg gagacctctc
      481 agcctgaggg tgaagcagga gtcggggtgg agagcaactc cgatggggcc tccccggagc
      541 cctgcaccgt cacccctggt gccgtgaagc tggagaagga gaagctggag caaaacccgg
      601 aggagtccca ggacatcaaa gctctgcaga aagaactcga gcaatttgcc aagctcctga
      661 agcagaagag gatcaccctg ggatatacac aggccgatgt ggggctcacc ctgggggttc
      721 tatttgggaa ggtattcagc caaacgacca tctgccgctt tgaggctctg cagcttagct
      781 tcaagaacat gtgtaagctg cggcccttgc tgcagaagtg ggtggaggaa gctgacaaca
      841 atgaaaatct tc
//
