LOCUS       sequence-35844-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_OCT4
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    57..105
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     130..149
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
       61 aaacgaccca acacccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc
      121 cttccggtat tgtctccttc cgtgtttcag ttagcctccc ccgtttaaac tcattactaa
      181 ccggtaggga tcgaaccctt gcgtcgacac tagttctaga ccctcacttc ctactcaggc
      241 tttattcaaa gaccaagagg tacaggtgca agggagagaa gaagggcatg gccagaaggc
      301 aagccccgca gaaggcagct cagtttgaat gcatgggaga gcccagagtg gtgacagaga
      361 cagggggaaa ggcttccccc tcagggaaag ggaccgagga gtacagtgca gtgaagtgag
      421 ggctcccata gcctggggta ccaaaatggg gccctggggc cagaggaaag gacactggtc
      481 cccctgagaa aggagaccca gcagcctcaa aatcctctcg ttgtgcatag tcgctgcttg
      541 atcgcttgcc cttctggcgc cggttacaga accacactcg gaccacatcc ttctcgagcc
      601 caagctgctg ggcgatgtgg ctgatctgct gcagtgtggg tttcgggcac tgcaggaaca
      661 aattctccag gttgcctctc actcggttct cgatactggt tcgctttctc tttcgggcct
      721 gcacgagggt ttctgctttg catatctcct gaagattttc attgttgtca gcttcctcca
      781 cccacttctg cagcaagggc cgcagcttac acatgttctt gaagctaagc tgcagagcct
      841 caaagcggca gatggtcgtt tggctgaata ccttcccaaa tagaaccccc agggtgagcc
      901 ccacatcggc ctgtgtatat cccagggtga tcctcttctg cttc
//
