LOCUS       sequence-35847-e         573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 573)
  TITLE     Koloteva-Levine et al
REFERENCE   2  (bases 1 to 573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTH385-GST-TEV-SUP35
FEATURES             Location/Qualifiers
     source          1..573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..31
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             41..61
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaatttgt attttcaagg
       61 tatgtcggat tcaaaccaag gcaacaatca gcaaaactac cagcaataca gccagaacgg
      121 taaccaacaa caaggtaaca acagatacca aggttatcaa gcttacaatg ctcaagccca
      181 acctgcaggt gggtactacc aaaattacca aggttattct gggtaccaac aaggtggcta
      241 tcaacagtac aatcccgacg ccggttacca gcaacagtat aatcctcaag gaggctatca
      301 acagtacaat cctcaaggcg gttatcagca gcaattcaat ccacaaggtg gccgtggaaa
      361 ttacaaaaac ttcaactaca ataacaattt gcaaggatat caagctggtt tccaaccaca
      421 gtctcaaggt atgtctttga acgactttca aaagcaacaa aagcaggccg ctcccaaacc
      481 aaagaagact ttgaagcttg tctccagttc cggtatcaag ttggccaatg ctaccaagaa
      541 ggttggcaca aaacctgccg aatctgataa gaa
//
