LOCUS       sequence-358517-        9824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pCBh_NLS_TadA8e_dSiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector
            for encoding a human codon-optimized dSiCas12i-ABE8e driven by CBh 
            promoter and tagmCherry driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Zhang H, Kong X, Xue M, Hu J, Wang Z, Wei Y, Wang H, Zhou J, Zhang 
            W, Xu M, Shen X, Yin F, Ai Z, Huang G, Xia J, Song X, Li H, Yuan Y, 
            Li J, Zhong N, Zhang M, Zhou Y, Yang H
  TITLE     An engineered xCas12i with high activity, high specificity, and 
            broad PAM range.
  JOURNAL   Protein Cell. 2023 Jun 28;14(7):538-543. doi: 
            10.1093/procel/pwac052.
  PUBMED    37378659
REFERENCE   2  (bases 1 to 9824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Protein Cell"; date: "2023-06-28- 28"; volume: "14"; issue: "7"; 
            pages: "538-543"
COMMENT     Sequence Label: HEpl017
FEATURES             Location/Qualifiers
     source          1..9824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        268..343
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        440..725
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        727..1004
                     /label=chicken beta-actin promoter
     intron          1005..1232
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             1286..1306
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5144..5191
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5219..5236)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5225..5432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        5501..5804
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5805..6008
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5959..5979
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6037..6747
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6182..6200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     rep_origin      7302..7757
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7389..7408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7599..7620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7774..7793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7893..7915
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7953..7971)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8039..8143
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8144..8212,8213..9004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8362..8381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9175..9763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9664..9683
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
      301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      361 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
      541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt
      661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
     1021 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
     1081 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
     1141 tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg tttaattacc
     1201 tggagcacct gcctgaaatc actttttttc aggttggacc ggtgccacca tgaaacggac
     1261 agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtctctg aggtggagtt
     1321 ttcccacgag tactggatga gacatgccct gaccctggcc aagagggcac gggatgagag
     1381 ggaggtgcct gtgggagccg tgctggtgct gaacaataga gtgatcggcg agggctggaa
     1441 cagagccatc ggcctacacg acccaacagc ccatgccgaa attatggccc tgagacaagg
     1501 cggcctggtc atgcagaact acagactgat tgacgccacc ctgtacgtga cattcgagcc
     1561 ttgcgtgatg tgcgctggcg ccatgatcca ctctaggatc ggccgcgtgg tgtttggatg
     1621 gagaaattct aaaagaggcg ccgcaggctc cctgatgaac gtgctgaact accccggcat
     1681 gaatcaccgc gtcgaaatta ccgagggaat cctggcagat gaatgtgccg ccctgctgtg
     1741 cgacttctat cggatgccta gacaggtgtt caatgctcag aagaaggccc agagttccat
     1801 caactccgga ggatctagcg gaggctcctc tggcagcgag acaccaggaa caagcgagtc
     1861 agcaacacca gagagcagtg gcggcagcag cggcggcagc agtatgagtt ctgatgtggt
     1921 gcggccttat aacacaaagc tgctcccaga taacagaaag cacaatatgt tcctgcagac
     1981 cttcaagcgg ctgaacagca tctctctgaa ccacttcgac ctgctgatct gcctgtacgc
     2041 tgcaatcacc aacaagaagg ccgaggaata caagtctgaa aaggaagccc acgtgaccgc
     2101 cgatagcctg tgtgccatca attggttcag acccatgagc aagagataca gcaaatacgc
     2161 caccaccacc ttcaacatgt tagaactgtt taaggagtac agcggccacg agcctgatgc
     2221 ctattccaag aactacctga tgagcaatat cgacagcgac agattcgtgt gggtggattg
     2281 taggaagttc gctaaggact ttgcctatca gatggaactg ggtttccacg agttcaccgt
     2341 gttggccgaa accctgctgg ctaattctat cctggtgctg aacgagagca ccaaggccaa
     2401 ttgggcttgg ggaaccgtgt ctgccctgta cggcggcgga gataaggagg acagcacact
     2461 gaagagcaag attctgctgg ccttcgtgga cgccctgaac aaccacgagc tgaaaacaaa
     2521 gagagaaatc ttgaatcaag tgtgtgaatc tctgaaatac cagagctacc aggacatgta
     2581 cgtggatttt agaagcgtgg ttgacgaaaa cggcaacaag aagtctccta acggctctat
     2641 gcctatcgtg accaagttcg agacagacga cctgatcagc gacaaccaaa gaaaggccat
     2701 gatcagcaac ttcactaaga acgccgctgc caaggcagct aagaaaccta tcccttactt
     2761 ggaccgcctg aaggagcaca tggtgtccct gtgcgacgag tacaatgtgt atgcctgggc
     2821 cgcggccatc acaaacagca acgccgacgt gaccgcccgg aataccagaa acctgacatt
     2881 catcggcgaa cagaacagca gacgaaagga actgagcgtg ctgcagacaa caaccaacga
     2941 gaaggctaag gacatcctga acaagatcaa cgacaacctg attcaggagg tgcggtacac
     3001 ccctgcccct aagcacctgg gcagagatct ggccaacctg tttgatacac tgaaggaaaa
     3061 ggacatcaac aacatcgaga acgaagaaga gaaacagaac gtgatcaatg actgtatcga
     3121 gcagtacgtg gacgattgca gaagcctcaa ccggaacccc atcgcagccc tcctgaagca
     3181 catctctagg tactacgagg atttcagcgc caagaatttc ctggacggcg ccaagctgaa
     3241 cgtgctgact gaggtggtga accggcagaa ggcccacccc accatctgga gcgagaaggc
     3301 ttacacctgg atcagcaagt tcgacaagaa ccggagacag gccaacagca gcctggtcgg
     3361 atgggttgtg ccccccgagg aggtgcacaa ggagaaaatc gccggacagc agagcatgat
     3421 gtgggtgacc ctcaccctgc tggacgacgg caagtgggtc aaacatcaca tccccttcag
     3481 cgacagcaga tactacagcg aagtgtacgc ctacaaccct aatctgcctt atctggacgg
     3541 aggcatccca agacagagca agttcggcaa caaaccaaca accaacctga cagccgagtc
     3601 ccaggccctc ctggctaatt ctaagtacaa gaaagccaac aagagcttcc tgcgggctaa
     3661 agagaatgcc acacacaacg tgcgggtgtc ccctaacacc tctctgtgca ttagactgct
     3721 gaaggacagc gccggaaacc agatgttcga caaaatcggc aacgtgctct tcggcatgca
     3781 gatcaaccac aagatcaccg tgggaaaacc taactacaag atcgaggtgg gcgacagatt
     3841 cctgggcttc gatcagaacc agagcgagaa ccacacctac gccgtgctgc agagagtgtc
     3901 cgagagcagt cacgacaccc accactttaa cggctgggac gtgaaggtgc tggaaaaggg
     3961 caaagtgacc agcgatgtga tcgtgcggga cgaggtctac gaccaactgt cttacgaggg
     4021 cgtcccctac gatagcagca agttcgccga gtggcgggac aagcgcagaa gatttgtgct
     4081 tgagaacctg agcatccagc tggaagaggg caagaccttc ctgacagagt tcgacaagct
     4141 gaataaggac agcctgtacc gctggaacat gaactacctg aaactgctga gaaaggccat
     4201 ccgggccgga ggcaaagagt tcgccaagat cgctaagaca gagatcttcg agctggcggt
     4261 ggaaagattc ggccctatta acctgggcag cctgtcccag atcagcctta agatgattgc
     4321 ctcctttaag ggcgtggtcc agtcctactt ctccgtgagc ggctgcgtgg atgatgcctc
     4381 caaaaaggcc catgattcta tgctgttcac atttatgtgc gccgccgaag aaaagcggac
     4441 caacaagaga gaagaaaaga ccaacagagc cgccagcttt atcctgcaaa aagcctacct
     4501 gcatggctgc aagatgatcg tgtgcgagga cgaccttcct gtggccgacg gcaagacagg
     4561 caaagcccag aatgccgacc ggatggactg gtgcgccaga gccctggcca agaaggtgaa
     4621 cgacggctgt gttgccatga gcatctgcta cagagctatc cctgcctaca tgagcagcca
     4681 ccaggacccc tttgtgcaca tgcaggataa gaaaaccagc gtgctgcggc ctagattcat
     4741 ggaagttaat aaggatagca tcagagacta ccacgtggcg ggcctgagaa gaatgctgaa
     4801 cagcaagagt gacgctggca ccagtgttta ttaccggcaa gctgccctgc atttctgcga
     4861 agccctgggc gtgagccctg aactggtgaa aaacaagaaa acccacgccg ccgaactggg
     4921 caagcacatg ggcagcgcta tgctgatgcc ctggagaggc ggtagagtgt acatcgccag
     4981 caaaaagctg acctccgatg ccaaatcagt gaagtactgc ggcgaggata tgtggcagta
     5041 ccacgccgcc gagatcgccg ctgttaacat cgccatgtat gaggtgtgct gccagaccgg
     5101 cgctttcgga aagaaacaga aaaaatcgga cgagctgcct ggaaaaaggc cggcggccac
     5161 gaagaaggcc ggccaggcaa agaagaagaa gtaagaattc ctagagctcg ctgatcagcc
     5221 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5281 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5341 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5401 gattgggaag agaatagcag gcatgctggg gagcggccgc tagttattaa tagtaatcaa
     5461 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5521 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5581 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5641 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     5701 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     5761 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     5821 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     5881 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     5941 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     6001 gcagagctgg tttagtgaac cgtcagatcc gctagcatgg tgagcaaggg cgaggaggat
     6061 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     6121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     6181 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     6241 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     6301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     6361 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     6421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaaaac catgggctgg
     6481 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     6541 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     6601 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     6661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     6721 ggcggcatgg acgagctgta caagtaagga tccgggatgc agaaattgat gatctattaa
     6781 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga
     6841 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg
     6901 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt
     6961 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
     7021 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc
     7081 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
     7141 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
     7201 tccatcagaa gctggtcgac ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     7261 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     7321 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
     7381 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7441 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7501 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7561 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7621 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
     7681 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     7741 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7801 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7921 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7981 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8041 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8101 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8161 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8221 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8281 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8341 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8401 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8461 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8521 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8581 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8641 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8701 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8761 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8821 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
     8881 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8941 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9001 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     9361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
