LOCUS       sequence-358541-        5996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To produce cardiac specific adeno-associated virus expressing 
            activated human YAP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Lin Z, von Gise A, Zhou P, Gu F, Ma Q, Jiang J, Yau AL, Buck JN, 
            Gouin KA, van Gorp PR, Zhou B, Chen J, Seidman JG, Wang DZ, Pu WT
  TITLE     Cardiac-specific YAP activation improves cardiac function and 
            survival in an experimental murine MI model.
  JOURNAL   Circ Res. 2014 Jul 18;115(3):354-63. doi: 
            10.1161/CIRCRESAHA.115.303632. Epub 2014 May 15.
  PUBMED    24833660
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1161/CIRCRESAHA.115.303632"; journalName: "Circ Res"; date: 
            "2014-07-18- 18"; volume: "115"; issue: "3"; pages: "354-63"
COMMENT     Sequence Label: AAV9:cTNT::3Flag-hYAP S127A
FEATURES             Location/Qualifiers
     source          1..5996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1..133
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     116..135
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        178..196
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             214..285
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     polyA_signal    1815..1949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1839..1858)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1893..1912
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2062..2081)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2127..2182
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     repeat_region   2275..2404
                     /label=AAV2 ITR
     primer_bind     complement(2423..2440)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2423..2439)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2432..2454)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2581..3036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2668..2687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2878..2899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3062..3166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3167..3235,3236..4027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3385..3404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4198..4786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4687..4706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5074..5095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5110..5115,5116..5133,5134..5140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5148..5164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5153..5175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5172..5188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5172..5188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5226..5355
                     /label=AAV2 ITR
ORIGIN
        1 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
       61 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
      121 tttctctcca caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa
      181 tacgactcac tataggctag cctcgacacc atggactaca aggacgacga tgacaaggat
      241 tacaaagatg acgacgataa ggactataag gacgatgatg ataaactggt acctatggat
      301 cccgggcagc agccgccgcc tcaaccggcc ccccagggcc aagggcagcc gccttcgcag
      361 cccccgcagg ggcagggccc gccgtccgga cccgggcaac cggcacccgc ggcgacccag
      421 gcggcgccgc aggcaccccc cgccgggcat cagatcgtgc acgtccgcgg ggactcggag
      481 accgacctgg aggcgctctt caacgccgtc atgaacccca agacggccaa cgtgccccag
      541 accgtgccca tgaggctccg gaagctgccc gactccttct tcaagccgcc ggagcccaaa
      601 tcccactccc gacaggccag tactgatgca ggcactgcag gagccctgac tccacagcat
      661 gttcgagctc atgcctctcc agcttctctg cagttgggag ctgtttctcc tgggacactg
      721 acccccactg gagtagtctc tggcccagca gctacaccca cagctcagca tcttcgacag
      781 tcttcttttg agatacctga tgatgtacct ctgccagcag gttgggagat ggcaaagaca
      841 tcttctggtc agagatactt cttaaatcac atcgatcaga caacaacatg gcaggacccc
      901 aggaaggcca tgctgtccca gatgaacgtc acagccccca ccagtccacc agtgcagcag
      961 aatatgatga actcggcttc aggtcctctt cctgatggat gggaacaagc catgactcag
     1021 gatggagaaa tttactatat aaaccataag aacaagacca cctcttggct agacccaagg
     1081 cttgaccctc gttttgccat gaaccagaga atcagtcaga gtgctccagt gaaacagcca
     1141 ccacccctgg ctccccagag cccacaggga ggcgtcatgg gtggcagcaa ctccaaccag
     1201 cagcaacaga tgcgactgca gcaactgcag atggagaagg agaggctgcg gctgaaacag
     1261 caagaactgc ttcggcagga gttagccctg cgtagccagt taccaacact ggagcaggat
     1321 ggtgggactc aaaatccagt gtcttctccc gggatgtctc aggaattgag aacaatgacg
     1381 accaatagct cagatccttt ccttaacagt ggcacctatc actctcgaga tgagagtaca
     1441 gacagtggac taagcatgag cagctacagt gtccctcgaa ccccagatga cttcctgaac
     1501 agtgtggatg agatggatac aggtgatact atcaaccaaa gcaccctgcc ctcacagcag
     1561 aaccgtttcc cagactacct tgaagccatt cctgggacaa atgtggacct tggaacactg
     1621 gaaggagatg gaatgaacat agaaggagag gagctgatgc caagtctgca ggaagctttg
     1681 agttctgaca tccttaatga catggagtct gttttggctg ccaccaagct agataaagaa
     1741 agctttctta catggttata gagccctcag gcagactgaa ttctgcagat atccagcaca
     1801 gtggcggccg cgcggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     1861 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1921 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1981 aggttcaggg ggaggtgtgg gaggtttttt agtcgacccg ggcggcctcg aggacggggt
     2041 gaactacgcc tgaggatccg atctttttcc ctctgccaaa aattatgggg acatcatgaa
     2101 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     2161 ttggaatttt ttgtgtctct cactcggaag caattcgttg atctgaattt cgaccaccca
     2221 taatacccat taccctggta gataagtagc atggcgggtt aatcattaac tacaaggaac
     2281 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     2341 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     2401 gcagccttaa ttaacctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2461 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2521 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     2581 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2641 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2701 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2761 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2821 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2881 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2941 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3001 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     3061 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3181 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3241 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3301 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3361 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3421 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3481 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3541 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3601 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3661 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3721 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3781 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3841 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3901 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3961 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4021 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4081 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4141 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4321 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4861 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4921 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4981 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     5041 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5101 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5161 acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt aaggccttaa
     5221 ttaggctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga
     5281 cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca
     5341 tcactagggg ttccttgtag ttaatgatta acccgccatg ctacttatct accagggtaa
     5401 tggggatcct ctagaactat agctagaatt cgcccttacg ggccccccct cgaggtcggg
     5461 ataaaagcag tctgggcttt cacatgacag catctggggc tgcggcagag ggtcgggtcc
     5521 gaagcgctgc cttatcagcg tccccagccc tgggaggtga cagctggctg gcttgtgtca
     5581 gcccctcggg cactcacgta tctccgtccg acgggtttaa aatagcaaaa ctctgaggcc
     5641 acacaatagc ttgggcttat atgggctcct gtgggggaag ggggagcacg gagggggccg
     5701 gggccgctgc tgccaaaata gcagctcaca agtgttgcat tcctctctgg gcgccgggca
     5761 cattcctgct ggctctgccc gccccggggt gggcgccggg gggaccttaa agcctctgcc
     5821 ccccaaggag cccttcccag acagccgccg gcacccaccg ctccgtggga cgatccccga
     5881 agctctagag ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctt
     5941 ctgacacaac agtctcgaac ttaagctgca gaagttggtc gtgaggcact gggcag
//
