LOCUS       sequence-358565-        9147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-guide Perturb-seq vector backbone; modified bovine U6 promoter; 
            original constant region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9147)
  AUTHORS   Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE,
            Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit 
            A, Parnas O, Regev A, Weissman JS
  TITLE     A Multiplexed Single-Cell CRISPR Screening Platform Enables 
            Systematic Dissection of the Unfolded Protein Response.
  JOURNAL   Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 
            10.1016/j.cell.2016.11.048.
  PUBMED    27984733
REFERENCE   2  (bases 1 to 9147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11"; journalName: "Cell"; date: "2016-12-15- 
            15"; volume: "167"; issue: "7"; pages: "1867-1882.e21"
COMMENT     Sequence Label: pMJ114
FEATURES             Location/Qualifiers
     source          1..9147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        149..347
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     302..322
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             365..545
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    592..717
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1214..1447
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1632..1676
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1974..2091
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2150..2374
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2363..2380
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2424..3122)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             complement(3135..3188)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3198..3794)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3278..3298)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3775..3794
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          3815..4753
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(3838..3858)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(4754..4965)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    complement(5477..5510)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5566..6154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5619..5639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6037..6048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6157..6173)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6158..6174)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6683..6863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6925..7513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7005..7024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7684..8475,8476..8544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8307..8326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8545..8649)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8724..8743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8915..9147,1..147)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
       61 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      121 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      181 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      241 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      301 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
      361 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      421 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      481 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      541 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      601 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      661 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      721 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      781 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      841 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      901 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      961 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1021 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     1081 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1141 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1201 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1261 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1321 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1381 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1441 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1501 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1561 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1621 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1681 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1741 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1801 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1861 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1921 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     1981 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2041 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2101 tacagggaca gcagagatcc agtttggtta gtaccgggcc cgctctagac catagagccc
     2161 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
     2221 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
     2281 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
     2341 caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg
     2401 ggccctctag gccctaggaa ttcttaatta agcttgtgcc ccagtttgct agggaggtcg
     2461 cagtatctgg ccactgccac ctcgtgctgc tcgacgtagg tctcgttgtt ggcctccttg
     2521 attctttcca gtctgtagtc cacatagtag acgccaggca tcttgaggtt cttagcgggt
     2581 ttcttggatc tatatgtggt cttgatgttt gcgatcagat ggctcccgcc cacgagcttc
     2641 agggccatgt cgtttctgcc ttccaggccg ccgtcagcgg ggtacagcgt ctcggtgaag
     2701 gcctcccagc cgagtgtttt cttctgcatc acagggccgt tggatgtgaa gttcacccct
     2761 ctgatcttga cgttgtagat gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc
     2821 agcacgcccc cgtcttcgta tgtggtgact ctctcccatg tgaagccctc agggaaggac
     2881 tgcttgaaga agtcggggat gccctgggtg tggttgatga aggtcttgct gccgtagagg
     2941 aagctagtag ccaggatgtc gaaggcgaag gggagagggc cgccctcgac caccttgatt
     3001 ctcatggtct gggtgccctc gtagggcttg ccttcgccct cggatgtgca cttgaagtga
     3061 tggttgtcca cggtgccctc catgtacagc ttcatgtgca tgttctcctt aatcagctcg
     3121 ctggtggcga ccggtgggcc aggattctcc tcgacgtcac cgcatgttag cagacttcct
     3181 ctgccctctc ccgatccggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
     3241 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
     3301 ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
     3361 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc
     3421 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
     3481 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
     3541 cccgcttcga cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc
     3601 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
     3661 cgctcgatgt ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
     3721 gcggcgaggg tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc
     3781 ttgtactcgg tcatggtggg ctagctcacg acacctgaaa tggaagaaaa aaactttgaa
     3841 ccactgtctg aggcttgaga atgaaccaag atccaaactc aaaaagggca aattccaagg
     3901 agaattacat caagtgccaa gctggcctaa cttcagtctc cacccactca gtgtggggaa
     3961 actccatcgc ataaaacccc tccccccaac ctaaagacga cgtactccaa aagcgcgaga
     4021 actaatcgag gtgcctggac ggcgcccggt actccgtgga gtcacatgaa gcgacggctg
     4081 aggacggaaa ggcccttttc ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc
     4141 gcgtcctcca ttttgagctc cctgcagcag ggccgggaag cggccatctt tccgctcacg
     4201 caactggtgc cgaccgggcc agccttgccg cccagggcgg ggcgatacac ggcggcgcga
     4261 ggccaggcac cagagcaggc cggccagctt gagactaccc ccgtccgatt ctcggtggcc
     4321 gcgctcgcag gccccgcctc gccgaacatg tgcgctggga cgcacgggcc ccgtcgccgc
     4381 ccgcggcccc aaaaaccgaa ataccagtgt gcagatcttg gcccgcattt acaagactat
     4441 cttgccagaa aaaaagcgtc gcagcaggtc atcaaaaatt ttaaatggct agagacttat
     4501 cgaaagcagc gagacaggcg cgaaggtgcc accagattcg cacgcggcgg ccccagcgcc
     4561 caggccaggc ctcaactcaa gcacgaggcg aaggggctcc ttaagcgcaa ggcctcgaac
     4621 tctcccaccc acttccaacc cgaagctcgg gatcaagaat cacgtactgc agccaggtgg
     4681 aagtaattca aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac
     4741 acacggcact tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga
     4801 ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga
     4861 catcactttc ccagtttacc ccgcgccacc ttctctaggc accggttcaa ttgccgaccc
     4921 ctccccccaa cttctcgggg actgtgggcg atgtgcgctc tgcccactga cgggcacatg
     4981 catggcggta atacggttat ccacgcggcc gcctaatgga tcctagtact cgagcatcgt
     5041 gatagtcaga ctggaaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
     5101 tagccttatt taaacttgct atgctgtttc cagcttagct cttaaacggg tggtgcccat
     5161 cctggtccat aaacatggcc tctcaagaac tatataaagc taagaataga aaaaatttta
     5221 cagttatggt gactaaactg taaccctttt ttaagtttca aagtatataa taagttatga
     5281 ccttttgtac cacacacatt atcatcatat agtttgatta gggctaagtt taatagtctc
     5341 cataacctta ttacatcata tatgcaaatg aagggaacca tgggaaattg gccctaggcc
     5401 cgcccgacct ctgcgcgtct gtctagtgga ctgcgcgggc agacaagctc ggtcacggtt
     5461 aacgtcgagg gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa
     5521 gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca acctctggat
     5581 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5641 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5701 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5761 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5821 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5881 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5941 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6001 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6061 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6121 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga
     6181 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6241 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6301 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6361 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6421 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6481 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6541 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6601 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6661 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6841 gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6901 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6961 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7021 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7081 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7141 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7201 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7261 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7321 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7381 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7441 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7501 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7561 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7621 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7681 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7741 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7801 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7861 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7921 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7981 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8041 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8101 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8161 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8221 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8281 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8341 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8401 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8461 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8521 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8581 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8641 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8701 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     8761 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     8821 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     8881 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     8941 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     9001 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     9061 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     9121 tatttacggt aaactgccca cttggca
//
