LOCUS       sequence-358589-       10612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An improved agroinfection-compatible Tobacco mosaic virus 
            (TMV)-based transient expression vector that lacks the TMV CP gene 
            coding sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Lindbo JA
  TITLE     TRBO: a high-efficiency tobacco mosaic virus RNA-based 
            overexpression vector.
  JOURNAL   Plant Physiol. 2007 Dec;145(4):1232-40. Epub 2007 Aug 24.
  PUBMED    17720752
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007-12"; volume: "145(4)"; pages: "1232-40. Epub 
            2007 Aug 24"
COMMENT     Sequence Label: pJL-TRBO
FEATURES             Location/Qualifiers
     source          1..10612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     105..121
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     106..122
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        553..898
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     841..861
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    907..962
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    7269..7293
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7448..8596)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8895..9689)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9898..10533
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta aagtactggg gtggttttgg taccgggccc cccctcgagg tcgacggtat
      121 cgataagctt gatatcgaat tcctgcaggt caacatggtg gagcacgaca cacttgtcta
      181 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
      241 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
      301 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
      361 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
      421 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa
      481 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
      541 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
      601 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
      661 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
      721 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      781 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
      841 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agagggtatt
      901 tttacaacaa ttaccaacaa caacaaacaa cagacaacat tacaattact atttacaatt
      961 acaatggcat acacacagac agctaccaca tcagctttgc tggacactgt ccgaggaaac
     1021 aactccttgg tcaatgatct agcaaagcgt cgtctttacg acacagcggt tgaagagttt
     1081 aacgctcgtg accgcaggcc caaggtgaac ttttcaaaag taataagcga ggagcagacg
     1141 cttattgcta cccgggcgta tccagaattc caaattacat tttataacac gcaaaatgcc
     1201 gtgcattcgc ttgcaggtgg attgcgatct ttagaactgg aatatctgat gatgcaaatt
     1261 ccctacggat cattgactta tgacataggc gggaattttg catcgcatct gttcaaggga
     1321 cgagcatatg tacactgctg catgcccaac ctggacgttc gagacatcat gcggcacgaa
     1381 ggccagaaag acagtattga actatacctt tctaggctag agagaggggg gaaaacagtc
     1441 cccaacttcc aaaaggaagc atttgacaga tacgcagaaa ttcctgaaga cgctgtctgt
     1501 cacaatactt tccagacatg cgaacatcag ccgatgcagc aatcaggcag agtgtatgcc
     1561 attgcgctac acagcatata tgacatacca gccgatgagt tcggggcggc actcttgagg
     1621 aaaaatgtcc atacgtgcta tgccgctttc cacttctccg agaacctgct tcttgaagat
     1681 tcatgcgtca atttggacga aatcaacgcg tgtttttcgc gcgatggaga caagttgacc
     1741 ttttcttttg catcagagag tactcttaat tactgtcata gttattctaa tattcttaag
     1801 tatgtgtgca aaacttactt cccggcctct aatagagagg tttacatgaa ggagttttta
     1861 gtcaccagag ttaatacctg gttttgtaag ttttctagaa tagatacttt tcttttgtac
     1921 aaaggtgtgg cccataaaag tgtagatagt gagcagtttt atactgcaat ggaagacgca
     1981 tggcattaca aaaagactct tgcaatgtgc aacagcgaga gaatcctcct tgaggattca
     2041 tcatcagtca attactggtt tcccaaaatg agggatatgg tcatcgtacc attattcgac
     2101 atttctttgg agactagtaa gaggacgcgc aaggaagtct tagtgtccaa ggatttcgtg
     2161 ttcacagtgc ttaaccacat tcgaacatac caggcgaaag ctcttacata cgcaaatgtt
     2221 ttgtccttcg tcgaatcgat tcgatcgagg gtaatcatta acggtgtgac agcgaggtcc
     2281 gaatgggatg tggacaaatc tttgttacaa tccttgtcca tgacgtttta cctgcatact
     2341 aagcttgccg ttctaaagga tgacttactg attagcaagt ttagtctcgg ttcgaaaacg
     2401 gtgtgccagc atgtgtggga tgagatttcg ctggcgtttg ggaacgcatt tccctccgtg
     2461 aaagagaggc tcttgaacag gaaacttatc agagtggcag gcgacgcatt agagatcagg
     2521 gtgcctgatc tatatgtgac cttccacgac agattagtga ctgagtacaa ggcctctgtg
     2581 gacatgcctg cgcttgacat taggaagaag atggaagaaa cggaagtgat gtacaatgca
     2641 ctttcagaat tatcggtgtt aagggagtct gacaaattcg atgttgatgt tttttcccag
     2701 atgtgccaat ctttggaagt tgacccaatg acggcagcga aggttatagt cgcggtcatg
     2761 agcaatgaga gcggtctgac tctcacattt gaacgaccta ctgaggcgaa tgttgcgcta
     2821 gctttacagg atcaagagaa ggcttcagaa ggtgcattgg tagttacctc aagagaagtt
     2881 gaagaaccgt ccatgaaggg ttcgatggcc agaggagagt tacaattagc tggtcttgct
     2941 ggagatcatc cggaatcgtc ctattctaag aacgaggaga tagagtcttt agagcagttt
     3001 catatggcga cggcagattc gttaattcgt aagcagatga gctcgattgt gtacacgggt
     3061 ccgattaaag ttcagcaaat gaaaaacttt atcgatagcc tggtagcatc actatctgct
     3121 gcggtgtcga atctcgtcaa gatcctcaaa gatacagctg ctattgacct tgaaacccgt
     3181 caaaagtttg gagtcttgga tgttgcatct aggaagtggt taatcaaacc aacggccaag
     3241 agtcatgcat ggggtgttgt tgaaacccac gcgaggaagt atcatgtggc gcttttggaa
     3301 tatgatgagc agggtgtggt gacatgcgat gattggagaa gagtagctgt tagctctgag
     3361 tctgttgttt attccgacat ggcgaaactc agaactctgc gcagactgct tcgaaacgga
     3421 gaaccgcatg tcagtagcgc aaaggttgtt cttgtggacg gagttccggg ctgtggaaaa
     3481 accaaagaaa ttctttccag ggttaatttt gatgaagatc taattttagt acctgggaag
     3541 caagccgcgg aaatgatcag aagacgtgcg aattcctcag ggattattgt ggccacgaag
     3601 gacaacgtta aaaccgttga ttctttcatg atgaattttg ggaaaagcac acgctgtcag
     3661 ttcaagaggt tattcattga tgaagggttg atgttgcata ctggttgtgt taattttctt
     3721 gtggcgatgt cattgtgcga aattgcatat gtttacggag acacacagca gattccatac
     3781 atcaatagag tttcaggatt cccgtacccc gcccattttg ccaaattgga agttgacgag
     3841 gtggagacac gcagaactac tctccgttgt ccagccgatg tcacacatta tctgaacagg
     3901 agatatgagg gctttgtcat gagcacttct tcggttaaaa agtctgtttc gcaggagatg
     3961 gtcggcggag ccgccgtgat caatccgatc tcaaaaccct tgcatggcaa gatcctgact
     4021 tttacccaat cggataaaga agctctgctt tcaagagggt attcagatgt tcacactgtg
     4081 catgaagtgc aaggcgagac atactctgat gtttcactag ttaggttaac ccctacaccg
     4141 gtctccatca ttgcaggaga cagcccacat gttttggtcg cattgtcaag gcacacctgt
     4201 tcgctcaagt actacactgt tgttatggat cctttagtta gtatcattag agatctagag
     4261 aaacttagct cgtacttgtt agatatgtat aaggtcgatg caggaacaca atagcaatta
     4321 cagattgact cggtgttcaa aggttccaat ctttttgttg cagcgccaaa gactggtgat
     4381 atttctgata tgcagtttta ctatgataag tgtctcccag gcaacagcac catgatgaat
     4441 aattttgatg ctgttaccat gaggttgact gacatttcat tgaatgtcaa agattgcata
     4501 ttggatatgt ctaagtctgt tgctgcgcct aaggatcaaa tcaaaccact aatacctatg
     4561 gtacgaacgg cggcagaaat gccacgccag actggactat tggaaaattt agtggcgatg
     4621 attaaaagaa actttaacgc acccgagttg tctggcatca ttgatattga aaatactgca
     4681 tctttggttg tagataagtt ttttgatagt tatttgctta aagaaaaaag aaaaccaaat
     4741 aaaaatgttt ctttgttcag tagagagtct ctcaatagat ggttagaaaa gcaggaacag
     4801 gtaacaatag gccagctcgc agattttgat tttgtggatt tgccagcagt tgatcagtac
     4861 agacacatga ttaaagcaca acccaaacaa aagttggaca cttcaatcca aacggagtac
     4921 ccggctttgc agacgattgt gtaccattca aaaaagatca atgcaatatt cggcccgttg
     4981 tttagtgagc ttaccaggca attactggac agtgttgatt cgagcagatt tttgtttttc
     5041 acaagaaaga caccagcgca gattgaggat ttcttcggag atctcgacag tcatgtgccg
     5101 atggatgtct tggagctgga tatatcaaaa tacgacaaat ctcagaatga attccactgt
     5161 gcagtagaat acgagatctg gcgaagattg ggtttcgaag acttcttggg agaagtttgg
     5221 aaacaagggc atagaaagac caccctcaag gattataccg caggtataaa aacttgcatc
     5281 tggtatcaaa gaaagagcgg ggacgtcacg acgttcattg gaaacactgt gatcattgct
     5341 gcatgtttgg cctcgatgct tccgatggag aaaataatca aaggagcctt ttgcggtgac
     5401 gatagtctgc tgtactttcc aaagggttgt gagtttccgg atgtgcaaca ctccgcgaat
     5461 cttatgtgga attttgaagc aaaactgttt aaaaaacagt atggatactt ttgcggaaga
     5521 tatgtaatac atcacgacag aggatgcatt gtgtattacg atcccctaaa gttgatctcg
     5581 aaacttggtg ctaaacacat caaggattgg gaacacttgg aggagttcag aaggtctctt
     5641 tgtgatgttg ctgtttcgtt gaacaattgt gcgtattaca cacagttgga cgacgctgta
     5701 tgggaggttc ataagaccgc ccctccaggt tcgtttgttt ataaaagtct ggtgaagtat
     5761 ttgtctgata aagttctttt tagaagtttg tttatagatg gctctagttg ttaaaggaaa
     5821 agtgaatatc aatgagttta tcgacctgac aaaaatggag aagatcttac cgtcgatgtt
     5881 tacccctgta aagagtgtta tgtgttccaa agttgataaa ataatggttc atgagaatga
     5941 gtcattgtca ggggtgaacc ttcttaaagg agttaagctt attgatagtg gatacgtctg
     6001 tttagccggt ttggtcgtca cgggcgagtg gaacttgcct gacaattgca gaggaggtgt
     6061 gagcgtgtgt ctggtggaca aaaggatgga aagagccgac gaggccattc tcggatctta
     6121 ctacacagca gctgcaaaga aaagatttca gttcaaggtc gttcccaatt atgctataac
     6181 cacccaggac gcgatgaaaa acgtctggca agttttagtt aatattagaa atgtgaagat
     6241 gtcagcgggt ttctgtccgc tttctctgga gtttgtgtcg gtgtgtattg tttatagaaa
     6301 taatataaaa ttaggtttga gagagaagat tacaaacgtg agagacggag ggcccatgga
     6361 acttacagaa gaagtcgttg atgagttcat ggaagatgtc cctatgtcga tcaggcttgc
     6421 aaagtttcga tctcgaaccg gaaaaaagag tgatgtccgc aaagggaaaa atagtagtag
     6481 tgatcggtca gtgccgaaca agaactatag aaatgttaag gattttgggg gaatgagttt
     6541 taaaaagaat aatttaatcg atgatgattc ggaggctact gtcgccgaat cggattcgtt
     6601 ttaaatagat cttacagtat cactactcca tctcagttcg tgttcttgtc attaattaac
     6661 ggcctagggc ggccgcggtc ctgcaacttg aggtagtcaa gatgcataat aaataacgga
     6721 ttgtgtccgt aatcacacgt ggtgcgtacg ataacgcata gtgtttttcc ctccacttaa
     6781 atcgaagggt tgtgtcttgg atcgcgcggg tcaaatgtat atggttcata tacatccgca
     6841 ggcacgtaat aaagcgaggg gttcgaatcc ccccgttacc cccggtaggg gcccaggtac
     6901 ccggatgtgt tttccgggct gatgagtccg tgaggacgaa acctggctgc aggcatgcaa
     6961 gcttggcgta atcatggtca tagcctagag tccgcaaaaa tcaccagtct ctctctacaa
     7021 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt
     7081 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     7141 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac
     7201 ctgcagcccg gccgggggat ccactagcag attgtcgttt cccgccttca gtttaaacta
     7261 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     7321 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     7381 caaccacagg agatctcagt aaagcgctgg ctgctgaacc cccagccgga actgacccca
     7441 caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc
     7501 cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg
     7561 tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt
     7621 gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc
     7681 atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga
     7741 cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca
     7801 ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg
     7861 acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc
     7921 aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca
     7981 acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga
     8041 tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt
     8101 tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg
     8161 tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg
     8221 tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg
     8281 cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca
     8341 aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca
     8401 gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctgggcca
     8461 tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg
     8521 tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt
     8581 caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt
     8641 gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg
     8701 caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct
     8761 tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag
     8821 agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc
     8881 gccacatcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
     8941 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
     9001 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
     9061 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
     9121 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
     9181 tcgcgcggat ctttaaatgg ggtgtcttct tcccagtttt cgcaatccac atcggccaga
     9241 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
     9301 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
     9361 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
     9421 atgagcagat tgctccagcc atcatgccgt tcaaggtgca ggacctttgg aacaggcagc
     9481 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
     9541 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
     9601 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
     9661 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
     9721 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     9781 tctaaaactt taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     9841 acatagtatc gacggagccg attttgaaac cacaattatg ggtgatgctg caactcgagc
     9901 gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca
     9961 gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga
    10021 caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg
    10081 tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa
    10141 gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca
    10201 gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt
    10261 tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct
    10321 catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc
    10381 tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggccg gccgcggtgt
    10441 ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc
    10501 ccagcggcga gggcaaccag cccggtgagc gtctctagtg gactgatggg ctgcctgtat
    10561 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct gg
//
