LOCUS       sequence-358612-        9907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for KATNA1. Used for generating cell 
            lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible.
            Internal ID: WISP20-48..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9907)
  AUTHORS   Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, 
            Sundquist WI
  TITLE     Comprehensive analysis of the human ESCRT-III-MIT domain interactome
            reveals new cofactors for cytokinetic abscission.
  JOURNAL   Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.
  PUBMED    36107470
REFERENCE   2  (bases 1 to 9907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-15"; volume: "11:e77779. doi"; pages: " 
            10.7554/eLife.77779"
COMMENT     Sequence Label: pLVX-mCherry-KATNA1, V55D
FEATURES             Location/Qualifiers
     source          1..9907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2205..2519
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2284..2302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2320..2338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2492..2516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2533..2542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2539..3246
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2684..2702)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2952..2971
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        4739..5238
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4774..4797)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5171..5190
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5259..5858
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5259..5278)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5755..5775
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5875..6463
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5928..5948)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6346..6357)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6670..7303
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7431..7447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7431..7447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7444..7466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7455..7471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7479..7485,7486..7503,7504..7509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7524..7545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7662..7679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7833..8421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7913..7932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8592..9383,9384..9452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9215..9234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9453..9557)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9605..9739
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9642..9661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9696..9715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9907,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
     2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2521 ggagaaggat ccgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2581 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2641 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2701 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2761 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2821 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2881 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     2941 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     3001 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3061 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3241 tacaagggat ccatgagtct tcttatgatt agtgagaatg taaaattggc tcgtgaatat
     3301 gcattgctgg gaaactatga ctctgcgatg gtctattatc agggagttct tgaccaaatg
     3361 aacaagtatc tgtactcagt caaagataca tacctccagc agaaatggca acaggattgg
     3421 caggaaataa atgtggaagc taaacatgtt aaagatatca tgaaaacact agagagcttt
     3481 aaactggata gtacgccact caaagcggca cagcatgacc ttccagcttc tgagggagaa
     3541 gtctggtcca tgcctgtacc tgttgaacga agaccctcac caggacctag aaaacgccaa
     3601 tcttctcagt acagtgaccc taaatcacat ggtaatcgtc caagtacaac tgtcagagtt
     3661 caccgttcat ctgcacagaa tgttcacaat gacagaggga aagctgttcg ttgtcgtgaa
     3721 aagaaagaac agaataaagg aagagaggaa aagaacaaat cacctgctgc agtaacagaa
     3781 ccagagacaa ataaatttga tagtaccgga tatgataaag acttagtaga agctttggaa
     3841 agagatataa tttcccagaa tcccaatgtt cgatgggatg atatcgctga tttagtagaa
     3901 gctaaaaagt tgcttaagga agccgtagtg ttaccaatgt ggatgcccga attctttaag
     3961 ggcattagga gaccatggaa aggagtactg atggtcggcc cacctggcac ggggaagacg
     4021 ctccttgcta aagcagtagc tacagaatgc aagacaacat tcttcaatgt ctcttcatca
     4081 actttgactt ccaaatacag aggagaatct gagaagcttg ttcgtcttct gtttgaaatg
     4141 gctcgatttt attctccagc caccatattt attgatgaga tagactccat ctgtagtcgc
     4201 cgagggactt ctgaagaaca tgaagcaagc agaagggtga aagcggagct gctggttcag
     4261 atggatggtg ttggaggtac ttctgaaaat gatgaccctt ccaaaatggt tatggttctg
     4321 gcagctacta attttccctg ggatatagat gaggctttaa gacgacgcct tgagaaacga
     4381 atctatattc ctttgccgtc agcaaaaggc agggaggagc tattacgaat aagtctacgt
     4441 gagttggaat tggctgatga tgttgacctt gcaagtatag cagaaaacat ggaaggttat
     4501 tcaggtgcgg acattaccaa cgtgtgcagg gatgcgtcct tgatggcaat gagaaggcgc
     4561 attgaaggtt tgactccaga ggaaatccga aatctttcca aagaagaaat gcacatgcct
     4621 acaactatgg aggatttcga gatggcttta aaaaaggttt ctaagtcagt gtctgctgca
     4681 gacattgaaa gatacgagaa atggatattt gagtttggat catgctaaga attctaccgg
     4741 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     4801 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     4861 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     4921 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     4981 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5041 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5101 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5161 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5221 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     5281 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5341 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5401 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5461 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     5521 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     5581 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     5641 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     5701 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     5761 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     5821 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg ggcgcgtctg gaacaatcaa
     5881 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5941 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6001 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6061 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6121 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6181 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6241 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     6301 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6361 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6421 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca
     6481 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     6541 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     6601 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6661 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     6721 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     6781 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     6841 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     6901 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     6961 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7021 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7081 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7141 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7201 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7261 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7321 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga
     7381 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7441 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7501 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7561 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7621 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7681 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8341 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8461 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8521 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8581 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8641 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8701 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8761 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8821 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8881 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8941 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9001 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9061 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9121 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9181 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9241 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9301 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9361 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9421 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9481 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9541 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     9601 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9661 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9721 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9781 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9841 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9901 agtagtt
//
