LOCUS       sequence-358613-       11016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in rice plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Zong Y, Liu Y, Xue C, Li B, Li X, Wang Y, Li J, Liu G, Huang X, Cao 
            X, Gao C
  TITLE     An engineered prime editor with enhanced editing efficiency in 
            plants.
  JOURNAL   Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
  PUBMED    35332341
REFERENCE   2  (bases 1 to 11016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w."
COMMENT     Sequence Label: ePPE
FEATURES             Location/Qualifiers
     source          1..11016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2016..2036
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2037..4550,4551..4553,4554..6137)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6050..6058
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6360..6380
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8043..8063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8123..8299
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8853..8869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8853..8869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8866..8888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8877..8893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8901..8907,8908..8925,8926..8931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8946..8967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9084..9101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9255..9843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9335..9354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10014..10805,10806..10874))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10637..10656
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10875..10979)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaattcgag ctcggtacct gacccggtcg tgcccctctc tagagataat gagcattgca
       61 tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt
      121 tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta
      181 caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac
      241 aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc
      301 cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca
      361 tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc
      421 tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt
      481 tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat
      541 taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc
      601 cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga
      661 agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgatcga gagttccgct
      721 ccaccgttgg acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt
      781 gagccggcac ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc
      841 ctttcccacc gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc
      901 cacaccctct ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc
      961 cccaaatcca cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc
     1021 cctctctacc ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt
     1081 ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca
     1141 cggatgcgac ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg
     1201 ggaatcctgg gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt
     1261 ttcgttgcat agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt
     1321 ttgtcgggtc atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg
     1381 gcggtcgttc tagatcggag tagaattaat tctgtttcaa actacctggt ggatttatta
     1441 attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat
     1501 ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag
     1561 agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt
     1621 tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg
     1681 tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta
     1741 ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc
     1801 atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat
     1861 aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt
     1921 tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca
     1981 ccctgttgtt tggtgttact tctgcaaagc ttatgcctaa gaaaaagaga aaagtggaca
     2041 agaagtactc gatcggcctc gatattggga ctaactctgt tggctgggcc gtgatcaccg
     2101 acgagtacaa ggtgccctca aagaagttca aggtcctggg caacaccgat cggcattcca
     2161 tcaagaagaa tctcattggc gctctcctgt tcgacagcgg cgagacggct gaggctacgc
     2221 ggctcaagcg caccgcccgc aggcggtaca cgcgcaggaa gaatcgcatc tgctacctgc
     2281 aggagatttt ctccaacgag atggcgaagg ttgacgattc tttcttccac aggctggagg
     2341 agtcattcct cgtggaggag gataagaagc acgagcggca tccaatcttc ggcaacattg
     2401 tcgacgaggt tgcctaccac gagaagtacc ctacgatcta ccatctgcgg aagaagctcg
     2461 tggactccac agataaggcg gacctccgcc tgatctacct cgctctggcc cacatgatta
     2521 agttcagggg ccatttcctg atcgaggggg atctcaaccc ggacaatagc gatgttgaca
     2581 agctgttcat ccagctcgtg cagacgtaca accagctctt cgaggagaac cccattaatg
     2641 cgtcaggcgt cgacgcgaag gctatcctgt ccgctaggct ctcgaagtct cggcgcctcg
     2701 agaacctgat cgcccagctg ccgggcgaga agaagaacgg cctgttcggg aatctcattg
     2761 cgctcagcct ggggctcacg cccaacttca agtcgaattt cgatctcgct gaggacgcca
     2821 agctgcagct ctccaaggac acatacgacg atgacctgga taacctcctg gcccagatcg
     2881 gcgatcagta cgcggacctg ttcctcgctg ccaagaatct gtcggacgcc atcctcctgt
     2941 ctgatattct cagggtgaac accgagatta cgaaggctcc gctctcagcc tccatgatca
     3001 agcgctacga cgagcaccat caggatctga ccctcctgaa ggcgctggtc aggcagcagc
     3061 tccccgagaa gtacaaggag atcttcttcg atcagtcgaa gaacggctac gctgggtaca
     3121 ttgacggcgg ggcctctcag gaggagttct acaagttcat caagccgatt ctggagaaga
     3181 tggacggcac ggaggagctg ctggtgaagc tcaatcgcga ggacctcctg aggaagcagc
     3241 ggacattcga taacggcagc atcccacacc agattcatct cggggagctg cacgctatcc
     3301 tgaggaggca ggaggacttc taccctttcc tcaaggataa ccgcgagaag atcgagaaga
     3361 ttctgacttt caggatcccg tactacgtcg gcccactcgc taggggcaac tcccgcttcg
     3421 cttggatgac ccgcaagtca gaggagacga tcacgccgtg gaacttcgag gaggtggtcg
     3481 acaagggcgc tagcgctcag tcgttcatcg agaggatgac gaatttcgac aagaacctgc
     3541 caaatgagaa ggtgctccct aagcactcgc tcctgtacga gtacttcaca gtctacaacg
     3601 agctgactaa ggtgaagtat gtgaccgagg gcatgaggaa gccggctttc ctgtctgggg
     3661 agcagaagaa ggccatcgtg gacctcctgt tcaagaccaa ccggaaggtc acggttaagc
     3721 agctcaagga ggactacttc aagaagattg agtgcttcga ttcggtcgag atctctggcg
     3781 ttgaggaccg cttcaacgcc tccctgggga cctaccacga tctcctgaag atcattaagg
     3841 ataaggactt cctggacaac gaggagaatg aggatatcct cgaggacatt gtgctgacac
     3901 tcactctgtt cgaggaccgg gagatgatcg aggagcgcct gaagacttac gcccatctct
     3961 tcgatgacaa ggtcatgaag cagctcaaga ggaggaggta caccggctgg gggaggctga
     4021 gcaggaagct catcaacggc attcgggaca agcagtccgg gaagacgatc ctcgacttcc
     4081 tgaagagcga tggcttcgcg aaccgcaatt tcatgcagct gattcacgat gacagcctca
     4141 cattcaagga ggatatccag aaggctcagg tgagcggcca gggggactcg ctgcacgagc
     4201 atatcgcgaa cctcgctggc tcgccagcta tcaagaaggg gattctgcag accgtgaagg
     4261 ttgtggacga gctggtgaag gtcatgggca ggcacaagcc tgagaacatc gtcattgaga
     4321 tggcccggga gaatcagacc acgcagaagg gccagaagaa ctcacgcgag aggatgaaga
     4381 ggatcgagga gggcattaag gagctggggt cccagatcct caaggagcac ccggtggaga
     4441 acacgcagct gcagaatgag aagctctacc tgtactacct ccagaatggc cgcgatatgt
     4501 atgtggacca ggagctggat attaacaggc tcagcgatta cgacgtcgat gccatcgttc
     4561 cacagtcatt cctgaaggat gactccattg acaacaaggt cctcaccagg tcggacaaga
     4621 accggggcaa gtctgataat gttccttcag aggaggtcgt taagaagatg aagaactact
     4681 ggcgccagct cctgaatgcc aagctgatca cgcagcggaa gttcgataac ctcacaaagg
     4741 ctgagagggg cgggctctct gagctggaca aggcgggctt catcaagagg cagctggtcg
     4801 agacacggca gatcactaag cacgttgcgc agattctcga ctcacggatg aacactaagt
     4861 acgatgagaa tgacaagctg atccgcgagg tgaaggtcat caccctgaag tcaaagctcg
     4921 tctccgactt caggaaggat ttccagttct acaaggttcg ggagatcaac aattaccacc
     4981 atgcccatga cgcgtacctg aacgcggtgg tcggcacagc tctgatcaag aagtacccaa
     5041 agctcgagag cgagttcgtg tacggggact acaaggttta cgatgtgagg aagatgatcg
     5101 ccaagtcgga gcaggagatt ggcaaggcta ccgccaagta cttcttctac tctaacatta
     5161 tgaatttctt caagacagag atcactctgg ccaatggcga gatccggaag cgccccctca
     5221 tcgagacgaa cggcgagacg ggggagatcg tgtgggacaa gggcagggat ttcgcgaccg
     5281 tcaggaaggt tctctccatg ccacaagtga atatcgtcaa gaagacagag gtccagactg
     5341 gcgggttctc taaggagtca attctgccta agcggaacag cgacaagctc atcgcccgca
     5401 agaaggactg ggatccgaag aagtacggcg ggttcgacag ccccactgtg gcctactcgg
     5461 tcctggttgt ggcgaaggtt gagaagggca agtccaagaa gctcaagagc gtgaaggagc
     5521 tgctggggat cacgattatg gagcgctcca gcttcgagaa gaacccgatc gatttcctgg
     5581 aggcgaaggg ctacaaggag gtgaagaagg acctgatcat taagctcccc aagtactcac
     5641 tcttcgagct ggagaacggc aggaagcgga tgctggcttc cgctggcgag ctgcagaagg
     5701 ggaacgagct ggctctgccg tccaagtatg tgaacttcct ctacctggcc tcccactacg
     5761 agaagctcaa gggcagcccc gaggacaacg agcagaagca gctgttcgtc gagcagcaca
     5821 agcattacct cgacgagatc attgagcaga tttccgagtt ctccaagcgc gtgatcctgg
     5881 ccgacgcgaa tctggataag gtcctctccg cgtacaacaa gcaccgcgac aagccaatca
     5941 gggagcaggc tgagaatatc attcatctct tcaccctgac gaacctcggc gcccctgctg
     6001 ctttcaagta cttcgacaca actatcgatc gcaagaggta cacaagcact aaggaggtcc
     6061 tggacgcgac cctcatccac cagtcgatta ccggcctcta cgagacgcgc atcgacctgt
     6121 ctcagctcgg gggcgacgaa ttctccggga gcgagacgcc aggcacctcc gagtcggcca
     6181 ccccagaatc tgccacagtg gtgtccggcc aaaagcagga ccgccagggc ggagaacgca
     6241 gaaggtccca gctcgatagg gatcagtgtg cctactgcaa ggagaagggc cactgggcca
     6301 aagactgccc gaaaaagccg cgcggcccac gcggcccaag gccacaaaca tccctccttc
     6361 caaagaagaa gcggaaggtg gagctcagcg gaggatcttc cggaggatct agcggctccg
     6421 agacaccagg aacatccgaa agcgctacac cagaatctag cggaggctct tccggaggat
     6481 ctaggcctac cctcaacatc gaggatgagt atcgcctcca cgaaacctcc aaagaaccgg
     6541 acgtgtccct cggcagcaca tggctcagcg acttcccaca agcgtgggcc gaaaccggcg
     6601 gcatgggcct cgccgtccgc caagccccac tcattatccc gctgaaggcg acctccacac
     6661 cggtgtccat caagcagtac ccgatgagcc aagaggcgag gctcgggatt aagccgcaca
     6721 ttcagcgcct cctcgatcaa ggcattctcg tgccgtgcca atccccgtgg aatacaccac
     6781 tcctcccggt caaaaagccg ggcaccaacg actatcgccc ggtccaagat ctccgcgagg
     6841 tcaacaagcg cgtggaagac atccacccga ccgtcccgaa cccgtataat ctgctctccg
     6901 ggctcccacc atcccaccag tggtatacag tgctggacct caaagacgcc ttcttctgtc
     6961 tccgcctcca cccaacaagc cagccgctct tcgccttcga gtggcgcgac ccggagatgg
     7021 gcatctccgg ccaactgaca tggacacgcc tcccgcaagg cttcaagaac agcccgacac
     7081 tcttcaacga ggcgctccat agggacctcg cggattttcg catccagcat ccggacctca
     7141 tcctcctcca gtatgtggat gatctcctcc tcgccgcgac ctccgagctg gattgtcaac
     7201 aaggcacacg cgcgctcctc caaacactcg ggaacctcgg ctatcgcgcg tccgcgaaaa
     7261 aggcccaaat ctgccagaag caagtgaagt acctcgggta tctgctcaag gaaggccaac
     7321 gctggctcac cgaagcgcgc aaagaaacag tgatggggca accgacaccg aaaacaccac
     7381 gccagctgcg cgagtttctc ggcaaagccg gcttctgtcg cctcttcatc ccgggctttg
     7441 ccgagatggc cgcgccactc tacccactca ccaagccggg cacactgttt aactgggggc
     7501 cggatcagca gaaagcctac caagagatca aacaagcgct cctcaccgcc ccagcgctcg
     7561 ggctcccaga tctcacaaag ccgttcgagc tgttcgtcga tgagaagcaa ggctacgcga
     7621 agggcgtgct cacacagaag ctcggcccgt ggaggaggcc agtggcctat ctctccaaaa
     7681 aactcgatcc agtggccgcc ggctggccac cgtgtctgcg catggtcgcc gcgattgccg
     7741 tgctcacaaa ggatgccggc aaactcacaa tgggccagcc gctggtgatc ctcgcgccac
     7801 atgccgtgga agccctcgtc aaacagccgc cggataggtg gctctccaat gcgcgcatga
     7861 cccattacca agcgctcctc ctcgacaccg atcgcgtcca gttcggccca gtggtcgccc
     7921 tcaatccggc gacactgctg ccactcccag aggagggcct ccaacacaac tgtctggata
     7981 ttctcgcgga agcgcatggc acaaggccag acctcacaga tcaaccgctc agcggcggca
     8041 gcccgaagaa gaaaaggaag gtgtgagcgg ccgcggtacg ctgaaatcac cagtctctct
     8101 ctacaaatct atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag
     8161 ggaaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     8221 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc
     8281 cagtactaaa atccagatct cctaaagtcc ctatagatct ttgtcgtgaa tataaaccag
     8341 acacgagacg actaaacctg gagcccagac gccgttcgaa gctagaagta ccgcttaggc
     8401 aggaggccgt tagggaaaag atgctaaggc agggttggtt acgttgactc ccccgtaggt
     8461 ttggtttaaa tatgatgaag tggacggaag gaaggaggaa gacaaggaag gataaggttg
     8521 caggccctgt gcaaggtaag aagatggaaa tttgatagag gtacgctact atacttatac
     8581 tatacgctaa gggaatgctt gtatttatac cctatacccc ctaataaccc cttatcaatt
     8641 taagaaataa tccgcataag cccccgctta aaaattggta tcagagccat gaataggtct
     8701 atgaccaaaa ctcaagagga taaaacctca ccaaaatacg aaagagttct taactctaaa
     8761 gataaaagat ctttcaagat caaaactagt tccctcacac cggtgacggg gatcgcatgc
     8821 gatatctcga gatctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10981 acatttcccc gaaaagtgcc acctgccagt gccaag
//
