LOCUS       sequence-358615-       11080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in rice plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Zong Y, Liu Y, Xue C, Li B, Li X, Wang Y, Li J, Liu G, Huang X, Cao 
            X, Gao C
  TITLE     An engineered prime editor with enhanced editing efficiency in 
            plants.
  JOURNAL   Nat Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w.
  PUBMED    35332341
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Mar 24. doi: 10.1038/s41587-022-01254-w."
COMMENT     Sequence Label: ePPE-spG
FEATURES             Location/Qualifiers
     source          1..11080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2040..2060
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2067..3782
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6168..6215
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6438..6458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8121..8141
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8199..8375
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8929..8945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8929..8945)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8942..8964)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8953..8969
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8977..8983,8984..9001,9002..9007))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9022..9043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9160..9177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9331..9919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9411..9430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10090..10881,10882..10950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10713..10732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10951..11055)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccagtgcca agctaattcg agctcggtac ctgacccggt cgtgcccctc tctagagata
       61 atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt
      121 tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg aataatataa
      181 tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat
      241 ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt
      301 gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta
      361 ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca
      421 tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt
      481 tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac
      541 cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag
      601 cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt
      661 cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgatc
      721 gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg
      781 agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc
      841 tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat
      901 agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac
      961 aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc
     1021 tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg
     1081 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct
     1141 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt
     1201 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat
     1261 gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg
     1321 ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg
     1381 tggtctggtt gggcggtcgt tctagatcgg agtagaatta attctgtttc aaactacctg
     1441 gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg
     1501 aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg
     1561 atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg
     1621 tcgttcattc gttctagatc ggagtagaat actgtttcaa actacctggt gtatttatta
     1681 attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga
     1741 aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat
     1801 ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt ataataaaca
     1861 agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta
     1921 tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt
     1981 tgtcgatgct caccctgttg tttggtgtta cttctgcaaa gcttgtcgac ggatccatgc
     2041 caaagaagaa gaggaaggtt cttaaggaca agaagtactc gatcggcctc gacatcggga
     2101 cgaactcagt tggctgggcc gtgatcaccg acgagtacaa ggtgccctct aagaagttca
     2161 aggtcctggg gaacaccgac cgccattcca tcaagaagaa cctcatcggc gctctcctgt
     2221 tcgacagcgg ggagaccgct gaggctacga ggctcaagag aaccgctagg cgccggtaca
     2281 cgagaaggaa gaacaggatc tgctacctcc aagagatttt ctccaacgag atggccaagg
     2341 ttgacgattc attcttccac cgcctggagg agtctttcct cgtggaggag gataagaagc
     2401 acgagcggca tcccatcttc ggcaacatcg tggacgaggt tgcctaccac gagaagtacc
     2461 ctacgatcta ccatctgcgg aagaagctcg tggactccac cgataaggcg gacctcagac
     2521 tgatctacct cgctctggcc cacatgatca agttccgcgg ccatttcctg atcgaggggg
     2581 atctcaaccc agacaacagc gatgttgaca agctgttcat ccaactcgtg cagacctaca
     2641 accaactctt cgaggagaac ccgatcaacg cctctggcgt ggacgcgaag gctatcctgt
     2701 ccgcgaggct ctcgaagtcc aggaggctgg agaacctgat cgctcagctc ccaggcgaga
     2761 agaagaacgg cctgttcggg aacctcatcg ctctcagcct ggggctcacc ccgaacttca
     2821 agtcgaactt cgatctcgct gaggacgcca agctgcaact ctccaaggac acctacgacg
     2881 atgacctcga taacctcctg gcccagatcg gcgatcaata cgcggacctg ttcctcgctg
     2941 ccaagaacct gtcggacgcc atcctcctgt cagatatcct ccgcgtgaac accgagatca
     3001 cgaaggctcc actctctgcc tccatgatca agcgctacga cgagcaccat caggatctga
     3061 ccctcctgaa ggcgctggtc cgccaacagc tcccggagaa gtacaaggag attttcttcg
     3121 atcagtcgaa gaacggctac gctgggtaca tcgacggcgg ggcctcacaa gaggagttct
     3181 acaagttcat caagccaatc ctggagaaga tggacggcac ggaggagctc ctggtgaagc
     3241 tcaacaggga ggacctcctg cggaagcaga gaaccttcga taacggcagc atcccccacc
     3301 aaatccatct cggggagctg cacgccatcc tgagaaggca agaggacttc taccctttcc
     3361 tcaaggataa ccgggagaag atcgagaaga tcctgacctt cagaatccca tactacgtcg
     3421 gccctctcgc gcgggggaac tcaagattcg cttggatgac ccgcaagtct gaggagacca
     3481 tcacgccgtg gaacttcgag gaggtggtgg acaagggcgc tagcgctcag tcgttcatcg
     3541 agaggatgac caacttcgac aagaacctgc ccaacgagaa ggtgctccct aagcactcgc
     3601 tcctgtacga gtacttcacc gtctacaacg agctcacgaa ggtgaagtac gtcaccgagg
     3661 gcatgcgcaa gccagcgttc ctgtccgggg agcagaagaa ggctatcgtg gacctcctgt
     3721 tcaagaccaa ccggaaggtc acggttaagc aactcaagga ggactacttc aagaagatcg
     3781 agtgcttcga ttcggtcgag atcagcggcg ttgaggaccg cttcaacgcc agcctcggga
     3841 cctaccacga tctcctgaag atcatcaagg ataaggactt cctggacaac gaggagaacg
     3901 aggatatcct ggaggacatc gtgctgaccc tcacgctgtt cgaggacagg gagatgatcg
     3961 aggagcgcct gaagacgtac gcccatctct tcgatgacaa ggtcatgaag caactcaagc
     4021 gccggagata caccggctgg gggaggctgt cccgcaagct catcaacggc atccgggaca
     4081 agcagtccgg gaagaccatc ctcgacttcc tcaagagcga tggcttcgcc aacaggaact
     4141 tcatgcaact gatccacgat gacagcctca ccttcaagga ggatatccaa aaggctcaag
     4201 tgagcggcca gggggactcg ctgcacgagc atatcgcgaa cctcgctggc tcccccgcga
     4261 tcaagaaggg catcctccag accgtgaagg ttgtggacga gctcgtgaag gtcatgggcc
     4321 ggcacaagcc tgagaacatc gtcatcgaga tggccagaga gaaccaaacc acgcagaagg
     4381 ggcaaaagaa ctctagggag cgcatgaagc gcatcgagga gggcatcaag gagctggggt
     4441 cccaaatcct caaggagcac ccagtggaga acacccaact gcagaacgag aagctctacc
     4501 tgtactacct ccagaacggc agggatatgt acgtggacca agagctggat atcaaccgcc
     4561 tcagcgatta cgacgtcgat gccatcgttc cccagtcttt cctgaaggat gactccatcg
     4621 acaacaaggt cctcaccagg tcggacaaga accgcggcaa gtcagataac gttccatctg
     4681 aggaggtcgt taagaagatg aagaactact ggaggcagct cctgaacgcc aagctgatca
     4741 cgcaaaggaa gttcgacaac ctcaccaagg ctgagagagg cgggctctca gagctggaca
     4801 aggccggctt catcaagcgg cagctggtcg agaccagaca aatcacgaag cacgttgcgc
     4861 aaatcctcga ctctcggatg aacacgaagt acgatgagaa cgacaagctg atcagggagg
     4921 ttaaggtgat caccctgaag tctaagctcg tctccgactt caggaaggat ttccagttct
     4981 acaaggttcg cgagatcaac aactaccacc atgcccatga cgcttacctc aacgctgtgg
     5041 tcggcaccgc tctgatcaag aagtacccaa agctggagtc cgagttcgtg tacggggact
     5101 acaaggttta cgatgtgcgc aagatgatcg ccaagtcgga gcaagagatc ggcaaggcta
     5161 ccgccaagta cttcttctac tcaaacatca tgaacttctt caagaccgag atcacgctgg
     5221 ccaacggcga gatccggaag agaccgctca tcgagaccaa cggcgagacg ggggagatcg
     5281 tgtgggacaa gggcagggat ttcgcgaccg tccgcaaggt tctctccatg ccccaggtga
     5341 acatcgtcaa gaagaccgag gtccaaacgg gcgggttctc aaaggagtct atcctgccta
     5401 agcggaacag cgacaagctc atcgccagaa agaaggactg ggacccaaag aagtacggcg
     5461 ggttcctctg gcctaccgtg gcctactcgg tcctggttgt ggcgaaggtt gagaagggca
     5521 agtccaagaa gctcaagagc gtgaaggagc tcctggggat caccatcatg gagaggtcca
     5581 gcttcgagaa gaacccaatc gacttcctgg aggccaaggg ctacaaggag gtgaagaagg
     5641 acctgatcat caagctcccg aagtactctc tcttcgagct ggagaacggc aggaagagaa
     5701 tgctggcttc cgctaagcag ctccagaagg ggaacgagct cgcgctgcca agcaagtacg
     5761 tgaacttcct ctacctggct tcccactacg agaagctcaa gggcagcccg gaggacaacg
     5821 agcaaaagca gctgttcgtc gagcagcaca agcattacct cgacgagatc atcgagcaaa
     5881 tctccgagtt cagcaagcgc gtgatcctcg ccgacgcgaa cctggataag gtcctctccg
     5941 cctacaacaa gcaccgggac aagcccatca gagagcaagc ggagaacatc atccatctct
     6001 tcaccctgac gaacctcggc gctcctgctg ctttcaagta cttcgacacc acgatcgatc
     6061 ggaagcaata caggtccacg aaggaggtcc tggacgcgac cctcatccac cagtcgatca
     6121 ccggcctgta cgagacgagg atcgacctct cacaactcgg cggggataag agacccgcag
     6181 caaccaagaa ggcagggcaa gcaaagaaga agaagacgcg ttccgggagc gagacgccag
     6241 gcacctccga gtcggccacc ccagaatctg ccacagtggt gtccggccaa aagcaggacc
     6301 gccagggcgg agaacgcaga aggtcccagc tcgataggga tcagtgtgcc tactgcaagg
     6361 agaagggcca ctgggccaaa gactgcccga aaaagccgcg cggcccacgc ggcccaaggc
     6421 cacaaacatc cctccttcca aagaagaagc ggaaggtgga gctcagcgga ggatcttccg
     6481 gaggatctag cggctccgag acaccaggaa catccgaaag cgctacacca gaatctagcg
     6541 gaggctcttc cggaggatct aggcctaccc tcaacatcga ggatgagtat cgcctccacg
     6601 aaacctccaa agaaccggac gtgtccctcg gcagcacatg gctcagcgac ttcccacaag
     6661 cgtgggccga aaccggcggc atgggcctcg ccgtccgcca agccccactc attatcccgc
     6721 tgaaggcgac ctccacaccg gtgtccatca agcagtaccc gatgagccaa gaggcgaggc
     6781 tcgggattaa gccgcacatt cagcgcctcc tcgatcaagg cattctcgtg ccgtgccaat
     6841 ccccgtggaa tacaccactc ctcccggtca aaaagccggg caccaacgac tatcgcccgg
     6901 tccaagatct ccgcgaggtc aacaagcgcg tggaagacat ccacccgacc gtcccgaacc
     6961 cgtataatct gctctccggg ctcccaccat cccaccagtg gtatacagtg ctggacctca
     7021 aagacgcctt cttctgtctc cgcctccacc caacaagcca gccgctcttc gccttcgagt
     7081 ggcgcgaccc ggagatgggc atctccggcc aactgacatg gacacgcctc ccgcaaggct
     7141 tcaagaacag cccgacactc ttcaacgagg cgctccatag ggacctcgcg gattttcgca
     7201 tccagcatcc ggacctcatc ctcctccagt atgtggatga tctcctcctc gccgcgacct
     7261 ccgagctgga ttgtcaacaa ggcacacgcg cgctcctcca aacactcggg aacctcggct
     7321 atcgcgcgtc cgcgaaaaag gcccaaatct gccagaagca agtgaagtac ctcgggtatc
     7381 tgctcaagga aggccaacgc tggctcaccg aagcgcgcaa agaaacagtg atggggcaac
     7441 cgacaccgaa aacaccacgc cagctgcgcg agtttctcgg caaagccggc ttctgtcgcc
     7501 tcttcatccc gggctttgcc gagatggccg cgccactcta cccactcacc aagccgggca
     7561 cactgtttaa ctgggggccg gatcagcaga aagcctacca agagatcaaa caagcgctcc
     7621 tcaccgcccc agcgctcggg ctcccagatc tcacaaagcc gttcgagctg ttcgtcgatg
     7681 agaagcaagg ctacgcgaag ggcgtgctca cacagaagct cggcccgtgg aggaggccag
     7741 tggcctatct ctccaaaaaa ctcgatccag tggccgccgg ctggccaccg tgtctgcgca
     7801 tggtcgccgc gattgccgtg ctcacaaagg atgccggcaa actcacaatg ggccagccgc
     7861 tggtgatcct cgcgccacat gccgtggaag ccctcgtcaa acagccgccg gataggtggc
     7921 tctccaatgc gcgcatgacc cattaccaag cgctcctcct cgacaccgat cgcgtccagt
     7981 tcggcccagt ggtcgccctc aatccggcga cactgctgcc actcccagag gagggcctcc
     8041 aacacaactg tctggatatt ctcgcggaag cgcatggcac aaggccagac ctcacagatc
     8101 aaccgctcag cggcggcagc ccgaagaaga aaaggaaggt gtgagaattc ggtacgctga
     8161 aatcaccagt ctctctctac aaatctatct ctctctattt tctccataaa taatgtgtga
     8221 gtagtttccc gataagggaa attagggttc ttatagggtt tcgctcatgt gttgagcata
     8281 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     8341 tcctaaaacc aaaatccagt actaaaatcc agatctccta aagtccctat agatctttgt
     8401 cgtgaatata aaccagacac gagacgacta aacctggagc ccagacgccg ttcgaagcta
     8461 gaagtaccgc ttaggcagga ggccgttagg gaaaagatgc taaggcaggg ttggttacgt
     8521 tgactccccc gtaggtttgg tttaaatatg atgaagtgga cggaaggaag gaggaagaca
     8581 aggaaggata aggttgcagg ccctgtgcaa ggtaagaaga tggaaatttg atagaggtac
     8641 gctactatac ttatactata cgctaaggga atgcttgtat ttatacccta taccccctaa
     8701 taacccctta tcaatttaag aaataatccg cataagcccc cgcttaaaaa ttggtatcag
     8761 agccatgaat aggtctatga ccaaaactca agaggataaa acctcaccaa aatacgaaag
     8821 agttcttaac tctaaagata aaagatcttt caagatcaaa actagttccc tcacaccggt
     8881 gacggggatc gcatgcgata tctcgagatc tagcttggcg taatcatggt catagctgtt
     8941 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     9001 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     9061 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     9121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10021 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10081 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10141 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10201 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10261 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10321 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10381 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10441 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10501 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10561 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10621 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10681 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10741 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10801 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10861 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10921 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10981 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11041 aataggggtt ccgcgcacat ttccccgaaa agtgccacct
//
